FastQCFastQC Report
Fri 23 Jun 2023
DRR277080_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR277080_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40422424
Total Bases972.9 Mbp
Sequences flagged as poor quality0
Sequence length10-36
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAACCCCATTCGTGATGGGGATCGGGGATT21441465.304347903529981No Hit
AACTGTGGTAATTCTAGAGCT18066154.46933860275178No Hit
TTGGTGACTCTAGATAACCTCG17531674.3371149637142No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATT13345803.3015832994082692No Hit
TTGGTGACTCTAGATAACCTC12631743.1249338238597466No Hit
TTGGTGACTCTAGATAACCT12336563.0519099992617957No Hit
ACTGTGGTAATTCTAGAGCT11330542.8030332866727634No Hit
GAGGGCGCACCACCGGCCCGTCTCG4328581.070836325896735No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCG4128531.021346468484918No Hit
TTCTAGAGCT3029350.7494231419669438No Hit
GTTGGCCTCGGATAGCCGGTC2936010.726331998298766No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGAT2777230.6870518205439634No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCA2354240.582409407214174No Hit
GAGGGCGCACCACCGGCCCGTCTCGC2327050.5756829427151623No Hit
CTGTGGTAATTCTAGAGCT2323070.57469834070317No Hit
TAACTGTGGTAATTCTAGAGCT2175110.538094895051321No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCA2104310.5205798642852294No Hit
ATTGATCATCGACACTTCGAACG2089360.5168814220542538No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCC2074860.5132943041713679No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGAT2004380.49585843738613994No Hit
CAGTGGCTGGTTGAGAT1959680.4848002188092431No Hit
TTGGTGACTCTAGATAACCTCGG1809650.447684681156182No Hit
AACGATGCCGACCGGCGATGCGGCGGCG1792740.4435013595424164No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG1691190.41837916499020444No Hit
AACTGTGGTAATTCTAGAGCTA1688780.4177829612593248No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC1653840.40913924409877056No Hit
CACTCGCCGAATCCCGGGGCCGAGGGA1550660.3836138080190342No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAG1439870.3562057535193832No Hit
GGTTGGCCTCGGATAGCCGGTC1425420.35263100500850714No Hit
ATTGATCATCGACACTTCGAACGCA1398040.34585753689585763No Hit
AACTGTGGTAATTCTAGAGC1379230.34120417914571377No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC1344220.3325431448643456No Hit
TTTGGTGACTCTAGATAACCTCG1309720.3240082781774789No Hit
ACTGTGGTAATTCTAGAGC1273810.31512459519993163No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCC1048800.25945994728074695No Hit
ATGTACACTAGGGATAACAGGGTAATCAACGCGA1030850.25501934272917426No Hit
ATCGGGGATT978350.242031502118725No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATT959870.23745978222384684No Hit
TTTGGTGACTCTAGATAACCTC919040.22735895304052028No Hit
AACGATGCCGACCGGCGATGCGGCGGC919000.22734905754291232No Hit
TTGGTGACTCTAGATAACC910540.2252561597988285No Hit
AGGGCGCACCACCGGCCCGTCTCG883620.2185964899086705No Hit
TTGATCATCGACACTTCGAACG883240.21850248268139488No Hit
TGGTGACTCTAGATAACCTCG851390.21062319271105565No Hit
CACTCGCCGAATCCCGGGGCCGAGGG847150.20957426996461173No Hit
ACGATGCCGACCGGCGATGCGGCGGCG824340.20393136245367174No Hit
TTTCTAGAGCT787040.1947038109342478No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGA738390.1826684119685648No Hit
AGCTAGGAATAATGGAAT722500.17873742554380212No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG703690.1740840677936583No Hit
TTGATCATCGACACTTCGAACGCA702240.1737253560053697No Hit
TGGTGACTCTAGATAACCT693320.17151866003879432No Hit
GTCCGGTGCGGAGTGCCCTT669280.16557146597640954No Hit
TCGCTTCTGGCGCCAAGCGC668140.16528944429458264No Hit
TGGTGACTCTAGATAACCTC652410.16139803986025184No Hit
ACTCGCCGAATCCCGGGGCCGAGGGA649880.16077214963654826No Hit
CGAGGGCGCACCACCGGCCCGTCTCG646570.15995329720948948No Hit
ATGGGGATCGGGGATT631420.15620537749047408No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCG628420.155463215169877No Hit
CCGGGGTCCGGTGCGGAGTGCCCT614590.15204184687192435No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT603430.14928100303930314No Hit
AAACGATGCCGACCGGCGATGCGGCGGCG593070.14671806915884114No Hit
ATTGATCATCGACACTTCGAACGC584770.14466475340518917No Hit
TACCCTACGCCGGCAGGCGCGGGTA581780.14392506495899404No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGC566410.14012272000313492No Hit
AGCAGTGGCTGGTTGAGATTT564840.1397343217220224No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGC545900.1350488036046527No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAG545900.1350488036046527No Hit
TTTGGTGACTCTAGATAACCT543140.13436601426970338No Hit
ACGATGCCGACCGGCGATGCGGCGGC530430.13122171990477366No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAT517970.1281392723998937No Hit
TACCCTACGCCGGCAGGCGCGGGT508540.12580640883881677No Hit
GAGGGCGCACCACCGGCCCGTCTC470980.11651453658494107No Hit
TCGCTTCTGGCGCCAAGCGCCCGG469130.11605686982057285No Hit
CGGTTGGCCTCGGATAGCCGGTC460990.11404313605735271No Hit
GTCCGGTGCGGAGTGCCCT457100.11308079891497848No Hit
ATTGATCATCGACACTTCGAAC454930.11254396816974659No Hit
ACCGGCCCGTCTCG452760.11200713742451467No Hit
AGAGCAGTGGCTGGTTGAGATTTAA449120.11110664714219019No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCC448710.11100521829170858No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC448170.11087162907400111No Hit
TAACTGTGGTAATTCTAGAGCTA432700.10704454537412206No Hit
ATTCTAGAGCT421860.10436286552236451No Hit
TGCATTTATCAGTTGGTGACTCTAGATAACCTCG419800.10385324739555452No Hit
ACTCGCCGAATCCCGGGGCCGAGGG417400.10325951753907682No Hit
TTCGTGATGGGGATCGGGGATT413350.10225759840627073No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)