FastQCFastQC Report
Thu 22 Jun 2023
DRR395056_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR395056_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53019171
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length14-136
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGATTCATGATAACTC20101163.7913003204067453No Hit
CGTTCGAATTGTAGTCT15155832.858556577582098No Hit
CGTTCGAATTGTAGTCTG11647002.1967525671044537No Hit
TCTGATGATTCATGATAACTC5864451.1060999048815756No Hit
TTACGATAGGTGTCAAGTGGA5698281.0747584114432873No Hit
CTGATGATTCATGATAACTCG4930080.929867424747173No Hit
TTTACGATAGGTGTCAAGTGGA3964310.7477125585384954No Hit
CTGATGATTCATGATAACT3016420.568930057393768No Hit
CTGATGATTCATGATAACTCGACGGATCGC2849790.5375018028856016No Hit
GTTCGAATTGTAGTCT2368020.44663467107020594No Hit
TTGGGCCAACCTATGGTGTACCGGT2260480.4263514418209218No Hit
TGTGCTTGCTAAGTACATG2254610.42524429512487094No Hit
TACTTACTCCGTGAATC2252760.42489536473514455No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACCTT2075260.39141690842355875No Hit
GTTCGAATTGTAGTCTG2029960.38287282915079907No Hit
CGTTCGAATTGTAGTC1940900.36607513157834926No Hit
TCGTTCGAATTGTAGTCTG1881740.3549169035479638No Hit
TACTTACTCCGTGAATCG1874670.3535834236261446No Hit
CCTGATGATTCATGATAACTC1859300.35068447222609345No Hit
TCGTTCGAATTGTAGTCT1813130.3419763013646517No Hit
TTTGTCGCTAAGATTCGA1775530.33488452695723964No Hit
GTCGTTGTAGTATAGTGG1588050.2995237326513461No Hit
GCGGGTGCACCGCCGACCGACCTTG1523510.28735077732543196No Hit
TTTGCTTGCTAAGTAAATG1522910.2872376107125477No Hit
CGACCCTCAGTAGGAGCTT1480440.27922730063055873No Hit
TCTGATGATTCATGATAACTCG1466320.27656411300734973No Hit
TGATGATTCATGATAACTC1387240.2616487534292077No Hit
CGGGTGCACCGCCGACCGACCTTG1349010.25443815407826725No Hit
TCCGATGTCGTCCAGCGG1258970.23745561770477322No Hit
ATGGCTCATTAAATCAGTTATAGTTTGTTTGATG1214440.22905676891854831No Hit
TTACGATAGGTGTCAAGTGGAA1190060.224458432215019No Hit
GCGCTACACTGATGTATTCAACG1174690.22155948081496787No Hit
CCAGATTGAGGTTCTGGTC1148160.21655563041526998No Hit
ACTTACTCCGTGAATC1111700.20967887257233803No Hit
TTACTTACTCCGTGAATCG1044330.19697214805565333No Hit
GCGTCTGTAGTCCAACGG1042110.19655343158798164No Hit
GCATTTATTAGCTGATGATTCATGATAACTC1037210.1956292375827604No Hit
CTGATGATTCATGATAAC1008760.19026325402183297No Hit
CGAGGCAAACTCTGAATACTAGAT1005390.18962763487946652No Hit
TCGCGCTACACTGATGTATTCAACG965790.18215863842910707No Hit
CCTTTGTCGCTAAGATTCGA955340.18018765325470668No Hit
TTTTGTCGCTAAGATTCGA955210.1801631338219151No Hit
TTGTCGCTAAGATTCGA947870.17877872892429797No Hit
TCTACGAGTCGGGTTGTT937160.1767587048843144No Hit
TGGCTCCCAAAGGCACGT935340.17641543282523223No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACCTTG917390.1730298649897789No Hit
TGAGGCAAACTCTGAATACTAGAT914700.17252250134201458No Hit
CTGATGATTCATGATAACTCGACGGATCGCA903180.17034970237463726No Hit
TACTTACTCCGTGAATCGG897540.16928593621352547No Hit
TTACGATAGGTGTCAAGTGG839080.15825973589817163No Hit
TTACTTACTCCGTGAATC834520.15739966964025146No Hit
TTTTACTTACTCCGTGAATCGGA824250.1554626344497163No Hit
TCTGATGATTCATGATAACTCGACGGATCGC819980.15465726538802352No Hit
TCTGATGATTCATGATAACT812730.15328983548233902No Hit
CCAGATTTAGGCTCTGGTC795700.15007778978664152No Hit
TGGCTCCCAAAGGCACG784700.14800306855043058No Hit
CGACCCTCAGTAGGAGCT783560.14778805198595052No Hit
TTTACGATAGGTGTCAAGTGGAA750310.1415167355219492No Hit
TCCGTTGTAGTCTAGCTG740460.13965891696043306No Hit
TACGATAGGTGTCAAGTGGA727150.1371485042646178No Hit
CCAGACTCAAGTTCTGGTC721840.13614697974059234No Hit
TCGAGGCAAACTCTGAATACTAGAT705120.13299340346155167No Hit
TCCGTTGTAGTCTAGCTGG698760.1317938373649788No Hit
TTTACTTACTCCGTGAATCGG681500.12853841113434233No Hit
AAAAGCCAGTGGCGCGAAGCTACCGTGCGCTGG658940.12428334648989514No Hit
CTTTGTCGCTAAGATTCGA657160.12394761887167191No Hit
AGAATCCTTTGCAGACGACTTAAAT651090.12280274997132641No Hit
ACTTACTCCGTGAATCG648090.12223691690690525No Hit
ACGTTATTTTACTTACTCCGTGAATC646670.12196908925641255No Hit
CCTGCGGAAGGATCATTG644170.12149756170272824No Hit
TTTACTTACTCCGTGAATCG641000.12089966476465655No Hit
TCGGGTGCACCGCCGACCGACCTTG629860.11879853798543927No Hit
TTCGAATTGTAGTCT611080.11525642300216274No Hit
CTGATGATTCATGATAA606940.11447557337326153No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACCT599690.11310814346757704No Hit
GGCTCCCAAAGGCACGT593130.11187085516670942No Hit
TTACTTACTCCGTGAATCGG592710.11179163853769045No Hit
TCGACCCTCAGTAGGAGCTT592340.11172185245974517No Hit
CGAAATCCGCTAAGGAGTGTG590610.11139555539259563No Hit
GTGGACGTGCCGGAGTGG562730.10613708011390822No Hit
CTGATGATTCATGATAACTCGA558440.10532793883178596No Hit
CGCGCTACACTGATGTATTCAACG552290.10416798104972255No Hit
ATCTCTCAGGCGACGCCA548890.1035267035767119No Hit
TAGAATCCTTTGCAGACGACTTAAAT543350.10248179851774747No Hit
TCGCTAAGATTCGA542750.10236863190486323No Hit
TTTACGATAGGTGTCAAGTGG540760.10199329597213053No Hit
TTGATGATTCATGATAACTC537060.10129543519267775No Hit
TTCGAATTGTAGTCTG530700.10009586909610488No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)