FastQCFastQC Report
Thu 22 Jun 2023
DRR395057_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR395057_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68001645
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length14-134
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGATTCATGATAACTC24073843.540184946996503No Hit
CGTTCGAATTGTAGTCT12969451.9072259207847104No Hit
CGTTCGAATTGTAGTCTG9562381.4061983353490934No Hit
TCTGATGATTCATGATAACTC6814051.0020419358972859No Hit
CTGATGATTCATGATAACTCG6191060.9104279756761766No Hit
TACTTACTCCGTGAATC5095730.749353931070344No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACCTT4553680.6696426240865203No Hit
ACTTACTCCGTGAATC3261710.4796516319568446No Hit
GTTCGAATTGTAGTCT3251400.47813549216346163No Hit
CTGATGATTCATGATAACTCGACGGATCGC3015500.44344515489294417No Hit
CCAGATTGAGGTTCTGGTC2680950.3942478156226956No Hit
TGTGCTTGCTAAGTACATG2603860.38291132515985454No Hit
TACTTACTCCGTGAATCG2358010.3467577879917464No Hit
GTCGTTGTAGTATAGTGG2125540.31257185028391593No Hit
TTACGATAGGTGTCAAGTGGA2123830.3123203857789028No Hit
GCATTTATTAGCTGATGATTCATGATAACTC2104290.30944692587951367No Hit
GTTCGAATTGTAGTCTG2094380.3079896081925665No Hit
CCTGATGATTCATGATAACTC2088700.30715433428117805No Hit
TTGGGCCAACCTATGGTGTACCGGT2046460.3009427198415568No Hit
CTGATGATTCATGATAACT1925580.28316667927665573No Hit
TCTGATGATTCATGATAACTCG1920550.28242699128822546No Hit
AGAATCCTTTGCAGACGACTTAAAT1838620.2703787533375112No Hit
CGGGTGCACCGCCGACCGACCTTG1837590.27022728641343896No Hit
TTACTTACTCCGTGAATC1820910.2677744045750658No Hit
CCAGATTTAGGCTCTGGTC1793300.26371420867833417No Hit
TCGTTCGAATTGTAGTCT1757340.2584261013097551No Hit
ACGCATTTATTAGATA1755070.2580922858557319No Hit
TGGCTCCCAAAGGCACG1749190.2572276008911255No Hit
TGATGATTCATGATAACTC1743930.2564540901914947No Hit
TCGTTCGAATTGTAGTCTG1735180.2551673566132113No Hit
CGAGGCAAACTCTGAATACTAGAT1662070.24441614610940665No Hit
GCGCTACACTGATGTATTCAACG1659620.24405586070748728No Hit
TTTGCTTGCTAAGTAAATG1651490.24286030139417952No Hit
TGAGGCAAACTCTGAATACTAGAT1624430.2388809858937971No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACCTTG1616420.23770307321242007No Hit
CCAGACTCAAGTTCTGGTC1598420.23505607842280873No Hit
TACTTACTCCGTGAAT1563890.22997826008473765No Hit
TCCGATGTCGTCCAGCGG1557160.2289885781439552No Hit
TTTACGATAGGTGTCAAGTGGA1548390.22769890346035013No Hit
TTTGTCGCTAAGATTCGA1547460.22756214206288689No Hit
ATGGCTCATTAAATCAGTTATAGTTTGTTTGATG1510090.22206668676912153No Hit
TCGCGCTACACTGATGTATTCAACG1405230.20664647156697458No Hit
CGACCCTCAGTAGGAGCTT1399320.20577737494438553No Hit
TTACTTACTCCGTGAATCG1390040.2044127020750748No Hit
TTTTACTTACTCCGTGAATCGGA1288630.1894998275409367No Hit
GGCTCCCAAAGGCACG1201770.17672660712840108No Hit
TCGAGGCAAACTCTGAATACTAGAT1173920.17263111796780797No Hit
TCCGTTGTAGTCTAGCTG1171890.17233259577764626No Hit
GCGTCTGTAGTCCAACGG1151410.1693209039281329No Hit
TAGAATCCTTTGCAGACGACTTAAAT1132390.16652391276711026No Hit
ACTTACTCCGTGAAT1116520.1641901456942696No Hit
AAGCCATCTCGTACTAAACGA1114250.1638563302402464No Hit
TACTTACTCCGTGAATCGG1109790.16320046375348712No Hit
GCGGGTGCACCGCCGACCGACCTTG1103420.1622637217084969No Hit
CGTTCGAATTGTAGTC1095240.16106080963188465No Hit
ATGGCTCATTAAATCAGTTATAGTTTGTT1070610.1574388384280998No Hit
GGGCTCCCAAAGGCACG1032230.15179485731558406No Hit
CTGATGATTCATGATAACTCGACGGATCGCA1030160.15149045291477875No Hit
AAAAGCCAGTGGCGCGAAGCTACCGTGCGCTGG1029580.15140516086044684No Hit
TTGTCGCTAAGATTCGA984010.14470385238474745No Hit
ACTTACTCCGTGAATCG980720.14422004055931295No Hit
TTTACTTACTCCGTGAATCGG921470.13550701604350895No Hit
TTTACTTACTCCGTGAATCG912100.13412910820025015No Hit
TGGCTCCCAAAGGCACGT894040.13147329009467343No Hit
TCCGTTGTAGTCTAGCTGG890290.13092183284683775No Hit
ATCTCTCAGGCGACGCCA888250.13062184010401512No Hit
TCGGGTGCACCGCCGACCGACCTTG871480.1281557232916939No Hit
CGACCCTCAGTAGGAGCT871040.1280910189746145No Hit
TCTGATGATTCATGATAACTCGACGGATCGC869580.1278763182861238No Hit
TCGCTAAGATTCGA865600.12729103832708752No Hit
TTACTTACTCCGTGAAT813370.11961034177923197No Hit
CTGATGATTCATGAT800130.11766333005620673No Hit
TTTTGTCGCTAAGATTCGA799230.11753098031672617No Hit
TCCGTCCAAGGCTAAATACG789340.11607660373510083No Hit
TGTCGCTAAGATTCGA747660.10994734024448967No Hit
TTACTTACTCCGTGAATCGG742220.10914735959696269No Hit
CCTGCGGAAGGATCATTG737700.10848266979423807No Hit
TGTGCGTGCTAAGTACATG735210.10811650218167515No Hit
GGGGAGGTAGTGACAATAAATAACA728310.10710182084565749No Hit
CGCGCTACACTGATGTATTCAACG717630.10553127060382143No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)