FastQCFastQC Report
Thu 22 Jun 2023
DRR395058_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR395058_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72133821
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length14-134
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTCGAATTGTAGTCTG40814015.658096220911409No Hit
GCGGGTGCACCGCCGACCGACCTTG30881084.281081962925547No Hit
CGTTCGAATTGTAGTCT17248402.3911668286641854No Hit
TACTTACTCCGTGAATCGG8304471.151258852626149No Hit
CTGATGATTCATGATAACTC8089961.1215210684596897No Hit
CGGGTGCACCGCCGACCGACCTTG8054151.11655668427713No Hit
TCGTTCGAATTGTAGTCTG7853161.088693194278451No Hit
GCGTTCGAATTGTAGTCTG7689021.0659382649367763No Hit
TACTTACTCCGTGAATCGGA7449951.032795697873817No Hit
CTGATGATTCATGATAACTCG6144510.8518209509517041No Hit
TTTTTACTTACTCCGTGAATCGGA6123270.8488764237236234No Hit
TTTACGATAGGTGTCAAGTGGA5718940.7928236603465108No Hit
TTTTACTTACTCCGTGAATCGGA5662230.7849618835525155No Hit
TTACTTACTCCGTGAATCGGA5595920.7757692469944161No Hit
GCGGGTGCACCGCCGACCGACCTT4703060.6519909710591929No Hit
TTACTTACTCCGTGAATCGG4478390.62084469364239No Hit
GCGTTCGAATTGTAGTCT4320850.5990047303885372No Hit
TCGGGTGCACCGCCGACCGACCTTG4182660.5798472813467069No Hit
TTTACTTACTCCGTGAATCGGA3890320.5393198289052232No Hit
GTTCGAATTGTAGTCTG3692480.511893027266641No Hit
TACTTACTCCGTGAATCG3457550.4793243934769517No Hit
TTACGATAGGTGTCAAGTGGA3386660.4694968259063942No Hit
TTTACGATAGGTGTCAAGTGGAA3256050.4513902015533046No Hit
CGTTCGAATTGTAGTC3076920.42655719014247145No Hit
TCTGATGATTCATGATAACTC3046500.422340028264966No Hit
ACGCATTTATTAGATA2821070.39108839111683824No Hit
CGAGGGCACGTCTGCCTGGGTGTCACA2765700.38341238016491597No Hit
ATTTTACTTACTCCGTGAATCGGA2595700.35984507184223613No Hit
TCGTTCGAATTGTAGTCT2507210.3475775947041541No Hit
TGCGGGTGCACCGCCGACCGACCTTG2439110.3381368082525394No Hit
TCTGATGATTCATGATAACTCG2361790.32741784190248285No Hit
TTCGAATTGTAGTCTG2130710.29538293833068957No Hit
ACTTACTCCGTGAATCG2126490.2947979145593854No Hit
TTACTTACTCCGTGAATCG2080800.2884638538696016No Hit
TGTGCTTGCTAAGTACATG2080310.28839592456914215No Hit
TTTACTTACTCCGTGAATCGG2035310.2821575194249033No Hit
ACGTTATTTTACTTACTCCGTGAATCGGA2007240.2782661409271526No Hit
TTACGATAGGTGTCAAGTGGAA1995490.27663722402837915No Hit
TTTTACTTACTCCGTGAATCGG1958170.2714635066954238No Hit
AGGCTCCCAAAGGCACGT1929660.26751113045848496No Hit
CCAGATTGAGGTTCTGGTC1853250.25691831852356745No Hit
CTGATGATTCATGATAACTCGACGGATCGC1715670.23784543452924806No Hit
GCGGGTGCACCGCCGACCGACCT1688200.23403723476675387No Hit
TGAGGGCACGTCTGCCTGGGTGTCACA1591100.22057614277774082No Hit
TTTTTACTTACTCCGTGAATCGG1581800.2192868723812648No Hit
TTTGTCGCTAAGATTCGA1564020.21682200919316336No Hit
GTTCGAATTGTAGTCT1520990.2108567075630168No Hit
ACTTACTCCGTGAATCGG1504390.20855542922091982No Hit
TGAGGCAAACTCTGAATACTAGAT1477830.20487338387356466No Hit
CGAGGCAAACTCTGAATACTAGAT1461400.20259567283979038No Hit
CCGACCCTCAGTAGGAGCTT1459350.20231147882766393No Hit
TCGAGGCAAACTCTGAATACTAGAT1367440.18956988289861978No Hit
CGGGTGCACCGCCGACCGACCTT1356800.18809484666007087No Hit
TCGAATTGTAGTCTG1346050.18660456098672493No Hit
ACGTTATTTTACTTACTCCGTGAATCG1284650.17809260374547467No Hit
GTCGTTGTAGTATAGTGG1269690.17601868061307885No Hit
CCAGATTTAGGCTCTGGTC1269160.17594520606360226No Hit
CTGATGATTCATGATAACT1195110.16567956382069376No Hit
TGCGTTCGAATTGTAGTCTG1194810.16563797445306552No Hit
TTTACTTACTCCGTGAATCG1186740.16451922046386536No Hit
TTTGCTTGCTAAGTAAATG1186070.16442633754282893No Hit
CCAGACTCAAGTTCTGGTC1184780.1642475032620274No Hit
CGTTCGAATTGTAGT1165510.16157607954803893No Hit
CGACCCTCAGTAGGAGCTT1136860.15760429493954023No Hit
CTGTTTTTGGTCCCAAGGCT1133940.15719949176129183No Hit
CTGATGATTCATGATAACTCGACGGATCGCA1129660.156606150116462No Hit
ACGTTATTTTACTTACTCCGTGAATC1126860.15621798268526493No Hit
CCGTTCGAATTGTAGTCTG1045830.1449846944888723No Hit
ACTTACTCCGTGAATC1036750.14372592296199033No Hit
AGGCTCCCAAAGGCACG1026650.14232574758517227No Hit
TTGTCGCTAAGATTCGA1005520.1393964697918886No Hit
CCTGATGATTCATGATAACTC973300.13492977170861364No Hit
TACTTACTCCGTGAATC973140.13490759071254524No Hit
AAGCCATCTCGTACTAAACGA936330.1298045753045579No Hit
TTTTACTTACTCCGTGAATCG913760.12667566854665857No Hit
TTTACGATAGGTGTCAAGTGG909050.1260227154748949No Hit
TTATTTTACTTACTCCGTGAATCGGA908430.12593676411512986No Hit
ACGTTATTTTACTTACTCCGTGAATCGG903930.12531292360070598No Hit
TGGCTCCCAAAGGCACGT901320.12495109610234012No Hit
TTTTTACTTACTCCGTGAATCGGAG900670.12486098580581223No Hit
TTTTGTCGCTAAGATTCGA890600.12346496936575702No Hit
TTGGGCCAACCTATGGTGTACCGGT883470.12247652872845874No Hit
ACTTACTCCGTGAATCGGA863050.1196456791052286No Hit
TCTGATGATTCATGATAACTCGACGGATCGCA861530.11943495964257876No Hit
CTGATGATTCATGATAACTCGA851770.11808191888240609No Hit
TTCGAATTGTAGTCT850020.1178393142379079No Hit
GCGGGTGCACCGCCGACCGACC816790.11323259861695113No Hit
TCTGATGATTCATGATAACTCGACGGATCGC790000.10951866808774763No Hit
TCCGTTGTAGTCTAGCTGG784940.10881719408708435No Hit
GCGTTCGAATTGTAGTC784260.10872292485379362No Hit
TATTTTACTTACTCCGTGAATCGGA778910.10798124779775635No Hit
CCTGATGATTCATGATAACTCG745330.10332601124789992No Hit
TCGCTAAGATTCGA741640.10281446202607236No Hit
TCCGTTGTAGTCTAGCTG733370.10166798179178668No Hit
CCTTTGTCGCTAAGATTCGA730360.10125070180324981No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)