FastQCFastQC Report
Thu 22 Jun 2023
DRR395059_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR395059_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51754888
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length14-135
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGGTGCACCGCCGACCGACCTTG27936115.39777228384689No Hit
CGTTCGAATTGTAGTCTG19185373.7069677360716153No Hit
TACTTACTCCGTGAATCGGA6932711.33952758239956No Hit
ACGCATTTATTAGATA6376411.2320401504878147No Hit
CGGGTGCACCGCCGACCGACCTTG6224491.202686401330827No Hit
CTGATGATTCATGATAACTCG6221651.2021376608910834No Hit
TACTTACTCCGTGAATCGG5084400.9823999619127763No Hit
GCGTTCGAATTGTAGTCTG4628380.8942884776409912No Hit
TTACTTACTCCGTGAATCGGA4417360.853515517220325No Hit
CGTTCGAATTGTAGTCT4354870.8414412953613193No Hit
TCGTTCGAATTGTAGTCTG4146380.8011571776563404No Hit
CTGATGATTCATGATAACTC3796640.7335809518127061No Hit
TTTTTACTTACTCCGTGAATCGGA3667910.7087079388520752No Hit
CGAGGGCACGTCTGCCTGGGTGTCACA3254480.6288256289917968No Hit
TCGGGTGCACCGCCGACCGACCTTG3207760.6197984623210855No Hit
TCGAGGCAAACTCTGAATACTAGAT2974780.5747824244156416No Hit
TTTTACTTACTCCGTGAATCGGA2953190.5706108377628022No Hit
ACGTTATTTTACTTACTCCGTGAATCGGA2943480.5687346864705802No Hit
TTACTTACTCCGTGAATCGG2506610.4843233357977704No Hit
TTTACTTACTCCGTGAATCGGA2489290.4809767919891934No Hit
TCTGATGATTCATGATAACTCG2432620.4700271015947325No Hit
CGAGGCAAACTCTGAATACTAGAT2391090.462002738755806No Hit
TGCGGGTGCACCGCCGACCGACCTTG2233890.4316287961052104No Hit
ATTTTACTTACTCCGTGAATCGGA2046530.3954273845593097No Hit
AGGCTCCCAAAGGCACGT2012090.3887729406350952No Hit
GTTCGAATTGTAGTCTG1897720.366674544827534No Hit
CCAGATTGAGGTTCTGGTC1896600.3664581401470717No Hit
GCGGGTGCACCGCCGACCGACCTT1860430.3594694282789289No Hit
GCGTTCGAATTGTAGTCT1735490.33532871329950514No Hit
TACTTACTCCGTGAATCG1627960.3145519317904813No Hit
CTGATGATTCATGATAACTCGACGGATCGC1623780.3137442786080418No Hit
CTGTTTTTGGTCCCAAGGCT1412180.27285925147785073No Hit
TGTGCTTGCTAAGTACATG1401040.27070679778111006No Hit
TCTGATGATTCATGATAACTC1368090.2643402493692963No Hit
CCGACCCTCAGTAGGAGCTT1364270.26360215483414823No Hit
TTTACGATAGGTGTCAAGTGGA1311100.25332872906613185No Hit
CCAGATTTAGGCTCTGGTC1303840.25192596301242115No Hit
CTGATGATTCATGATAACTCGACGGATCGCA1196140.23111633436439857No Hit
TTCGAATTGTAGTCTG1163730.22485412392352194No Hit
TTTGTCGCTAAGATTCGA1155540.2232716646976417No Hit
CCAGACTCAAGTTCTGGTC1144390.22111727881625404No Hit
TGAGGCAAACTCTGAATACTAGAT1129760.21829049267771578No Hit
TGAGGGCACGTCTGCCTGGGTGTCACA1077970.20828370839098329No Hit
TACTTACTCCGTGAATCGGAGGCGGGG1072530.20723259994302373No Hit
TCTGATGATTCATGATAACTCGACGGATCGCA1071280.20699107686215065No Hit
TTTACGATAGGTGTCAAGTGGAA1008120.19478739863179687No Hit
AAGCCATCTCGTACTAAACGA992620.19179251242897097No Hit
TTTACTTACTCCGTGAATCGG977820.18893287915143397No Hit
TTACTTACTCCGTGAATCG975530.18849040886727453No Hit
GTCGTTGTAGTATAGTGG957410.1849892902869387No Hit
CGCGGGTGCACCGCCGACCGACCTTG944980.18258758477073703No Hit
AGGCTCCCAAAGGCACG914390.17667703193561157No Hit
TTATTTTACTTACTCCGTGAATCGGA887980.17157413228292562No Hit
TCGAATTGTAGTCTG876490.1693540521235405No Hit
TTTTTACTTACTCCGTGAATCGGAG843290.1629391990955521No Hit
TTGTCGCTAAGATTCGA832340.16082345690710412No Hit
CGACCCTCAGTAGGAGCTT821610.1587502227808898No Hit
TTTTACTTACTCCGTGAATCGG815050.157482709652468No Hit
TGAAGGTCACGGCGAGACGAGCCGTTTA813240.15713298423136382No Hit
ACTTACTCCGTGAATCG801920.1549457512109774No Hit
TCGAGGGCACGTCTGCCTGGGTGTCACA784020.15148714069287522No Hit
CTGATGATTCATGATAACTCGA764450.14770585533872665No Hit
ACGTTATTTTACTTACTCCGTGAATCGG740870.14314976394113732No Hit
ACTTACTCCGTGAATCGG733070.14164265991648942No Hit
CCTGATGATTCATGATAACTCG729960.14104175049127726No Hit
TCTGATGATTCATGATAACTCGACGGATCGC729650.14098185276722075No Hit
TATTTTACTTACTCCGTGAATCGGA719790.1390767187052941No Hit
TTTGCTTGCTAAGTAAATG719300.13898204165759184No Hit
TTTTTACTTACTCCGTGAATCGG717380.13861106220537084No Hit
TTACTTACTCCGTGAATCGGAGGCGGGG710410.13726432950642264No Hit
TCGTTCGAATTGTAGTCT697340.13473896417281397No Hit
AAAAATCCGGAGGACCGAG679250.1312436421464191No Hit
GCGGGTGCACCGCCGACCGACCT677920.13098666158837016No Hit
TTTTGTCGCTAAGATTCGA677470.13089971327925587No Hit
TCGCTAAGATTCGA675090.13043985333327357No Hit
TGCGTTCGAATTGTAGTCTG670560.1295645736881896No Hit
TCGCGGGTGCACCGCCGACCGACCTTG663860.12827000997471003No Hit
TTACGATAGGTGTCAAGTGGA650120.12561518826975338No Hit
TGGCTCCCAAAGGCACGT645620.12474570517861039No Hit
ACTTACTCCGTGAATCGGA642410.12412547390692837No Hit
GTCGTTGTAGTATAGTGGT637270.12313233099837835No Hit
CAGTGGCTGGTTGAGAT631300.12197881676412864No Hit
ACGTTATTTTACTTACTCCGTGAATCG628960.12152668555673427No Hit
GCGGGTGCACCGCCGATCGACCTTG617540.11932013068987804No Hit
GGGTGCACCGCCGACCGACCTTG598670.11567409826101836No Hit
ATTTTACTTACTCCGTGAATCGGAG589040.11381340444597234No Hit
TCCGTTGTAGTCTAGCTGG574800.11106197350866646No Hit
CGTTCGAATTGTAGTC570430.11021760881793426No Hit
TGTCGCTAAGATTCGA565400.10924571994050107No Hit
TTTTACTTACTCCGTGAATCGGAG552400.10673387989942129No Hit
TACTTACTCCGTGAATC542390.10479976306778985No Hit
TTACGATAGGTGTCAAGTGGAA538670.10408099037911163No Hit
GGTGCACCGCCGACCGACCTTG519170.10031323031749195No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)