FastQCFastQC Report
Thu 22 Jun 2023
DRR395060_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR395060_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51976119
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length14-135
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGGTGCACCGCCGACCGACCTTG14592802.8075970812672644No Hit
CTGATGATTCATGATAACTC7015121.349681379635136No Hit
CTGATGATTCATGATAACTCG4978690.9578802911390902No Hit
TTTTTACTTACTCCGTGAATCGGA4302480.8277801580375787No Hit
CGGGTGCACCGCCGACCGACCTTG3786020.7284152939545178No Hit
TCTGATGATTCATGATAACTC3159160.6078099059300677No Hit
CCAGATTGAGGTTCTGGTC2921990.5621793347056173No Hit
CGTTCGAATTGTAGTCTG2715870.5225226608396829No Hit
TCGGGTGCACCGCCGACCGACCTTG2689120.5173760664970003No Hit
CCAGACTCAAGTTCTGGTC2380670.45803150481474No Hit
TTTTACTTACTCCGTGAATCGGA2355270.45314464513981895No Hit
AAAAGCCAGTGGCGCGAAGCTACCGTGCGCTGG2273800.43747013893053466No Hit
TCTGATGATTCATGATAACTCG2255560.43396083497500076No Hit
TGTGCTTGCTAAGTACATG2242270.43140389146792585No Hit
CCAGATTTAGGCTCTGGTC2094750.40302162614334486No Hit
CTGTTTTTGGTCCCAAGGCT1717570.3304536839312685No Hit
TGCGGGTGCACCGCCGACCGACCTTG1609280.309619115655788No Hit
TTTTTACTTACTCCGTGAATCGGAG1608630.30949405822316206No Hit
ATTTTACTTACTCCGTGAATCGGA1588220.3055672548387078No Hit
CCTGCGGAAGGATCATTG1477330.28423245683272347No Hit
GCGGGTGCACCGCCGACCGACCTT1456730.2802690981987324No Hit
TTTGCTTGCTAAGTAAATG1434460.2759844381609177No Hit
TGAGGCAAACTCTGAATACTAGAT1411920.2716478311895507No Hit
TTGGGCCAACCTATGGTGTACCGGT1410930.2714573590998589No Hit
TCGAGGCAAACTCTGAATACTAGAT1315480.25309315610886607No Hit
CGAGGCAAACTCTGAATACTAGAT1294790.2491124818303575No Hit
CTGATGATTCATGATAACTCGACGGATCGC1262440.2428884696065899No Hit
ATCTCTCAGGCGACGCCA1111280.2138058826593036No Hit
GCGTTCGAATTGTAGTCTG989830.19043938236327343No Hit
TTTTACTTACTCCGTGAATCGG953550.1834592536622444No Hit
TCGTTCGAATTGTAGTCTG923910.17775663473450182No Hit
CGTTCGAATTGTAGTCT922110.1774103218441531No Hit
ATTTTACTTACTCCGTGAATCGGAG911690.17540555500113428No Hit
TTTTTACTTACTCCGTGAATCGG911520.17537284767260133No Hit
ACGTTATTTTACTTACTCCGTGAATCGGA899430.17304677942575897No Hit
CCTGATGATTCATGATAACTC898390.17284668753355747No Hit
TTTACGATAGGTGTCAAGTGGA877690.168864089294547No Hit
CTGATGATTCATGATAACTCGACGGATCGCA876790.16869093284937262No Hit
ATGGATGGCGCTTAAGCGCGCGACC873200.16800023102917708No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACCTT865690.16655533669222206No Hit
CCAGTTTCAGGTACTGGTC820940.15794561344605204No Hit
TCCCCGGCAACGGCG779560.1499842648890349No Hit
ACCTGCGGAAGGATCATTG776920.1494763393165234No Hit
TTATTTTACTTACTCCGTGAATCGGA764250.1470386813605687No Hit
TTTGTCGCTAAGATTCGA728030.1400700964225513No Hit
TTTTACTTACTCCGTGAATCGGAG695450.13380183310723912No Hit
ATGGCTCATTAAATCAGTTATAGTTTGTTTGATG667960.1285128656874131No Hit
TTACGATAGGTGTCAAGTGGA661340.12723920383513052No Hit
AGAGGGTGAGAGCCCCGTCGTGCCCGG655720.12615793803304168No Hit
TGTGCGTGCTAAGTACATG654350.12589435544427624No Hit
CCTGATGATTCATGATAACTCG645270.12414739930851706No Hit
TCTGATGATTCATGATAACTCGACGGATCGC643150.12373951968210632No Hit
CCTTTGTCGCTAAGATTCGA642390.12359329868395906No Hit
GCATTTATTAGCTGATGATTCATGATAACTC635840.12233310455519004No Hit
TTTACTTACTCCGTGAATCGGA630790.12136150450171165No Hit
TTTATTTTACTTACTCCGTGAATCGGA627700.12076700070661296No Hit
TCTGATGATTCATGATAACTCGACGGATCGCA613760.11808499976691218No Hit
ACGTTATTTTACTTACTCCGTGAATC595240.11452182491732406No Hit
ACGTTATTTTACTTACTCCGTGAATCG590730.11365411873095026No Hit
CTGATGATTCATGATAACT584540.112463187180251No Hit
ATCCTGCCGTTCACGCCA575860.1107931894645693No Hit
AAAAATGGATGGCGCTTAAGCGCGCGACC574670.11056423816483874No Hit
CTGTTTTTGGTCCCAAGGC553450.10648159397972749No Hit
TGAATGCAGCCCCAATCGGGCGGTA528730.10172556361893814No Hit
TTTGGGCCAACCTATGGTGTACCGGT523280.10067700514538225No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)