FastQCFastQC Report
Thu 22 Jun 2023
DRR395061_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR395061_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28036792
Total Bases679.3 Mbp
Sequences flagged as poor quality0
Sequence length14-134
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGGTGCACCGCCGACCGACCTTG8600083.0674265443778306No Hit
CAGTGGCTGGTTGAGAT3260761.1630289228525148No Hit
CTGATGATTCATGATAACTCG3249171.158895069022162No Hit
CTGTTTTTGGTCCCAAGGCT2175690.7760124624814423No Hit
CTGATGATTCATGATAACTC2082980.7429451985804938No Hit
TCGAGGCAAACTCTGAATACTAGAT1685700.6012456774655246No Hit
CGGGTGCACCGCCGACCGACCTTG1518600.5416454207742455No Hit
TCTGATGATTCATGATAACTCG1425380.508396253037794No Hit
TTTTTACTTACTCCGTGAATCGGA1248040.4451436526689644No Hit
CGTTCGAATTGTAGTCTG1231820.43935839735159427No Hit
TGTGCTTGCTAAGTACATG1214290.43310589884891254No Hit
CGAGGCAAACTCTGAATACTAGAT1213690.43289189433655606No Hit
TCAGTGGCTGGTTGAGAT1204980.42978526216551455No Hit
GCGGGTGCACCGCCGACCGACCTT1122240.4002740399115562No Hit
TGCGGGTGCACCGCCGACCGACCTTG1062350.3789128228365071No Hit
GCGTTCGAATTGTAGTCTG976130.34816037441088127No Hit
CCAGATTGAGGTTCTGGTC962000.3431205681448862No Hit
TCTGATGATTCATGATAACTC907010.32350705458741497No Hit
CCAGACTCAAGTTCTGGTC902630.32194482164721266No Hit
ATTTTACTTACTCCGTGAATCGGA794930.2835310116792249No Hit
TCGGGTGCACCGCCGACCGACCTTG778750.27776002332934524No Hit
CCAGATTTAGGCTCTGGTC733150.2614956803902529No Hit
TTTGCTTGCTAAGTAAATG717610.2559529635202202No Hit
TGAGGCAAACTCTGAATACTAGAT676000.2411117505882984No Hit
ACGTTATTTTACTTACTCCGTGAATCGGA670980.2393212461682492No Hit
CCTGTTTTTGGTCCCAAGGCT567260.20232699946555938No Hit
ATCTCTCAGGCGACGCCA566810.20216649608129203No Hit
ATTTTACTTACTCCGTGAATCGGAG561070.20011918624641506No Hit
ATCCTGCCGTTCACGCCA551910.19685205069110617No Hit
CTGATGATTCATGATAACTCGACGGATCGCA490480.17494155536767544No Hit
CTGTTTTTGGTCCCAAGGC473470.1688745274423693No Hit
TTTTACTTACTCCGTGAATCGGA453060.16159480728037645No Hit
CTGATGATTCATGATAACTCGACGGATCGC449640.1603749815599445No Hit
TCGTTCGAATTGTAGTCTG448930.16012174288698935No Hit
CCTGCGGAAGGATCATTG445690.15896611852026438No Hit
TCTGATGATTCATGATAACTCGACGGATCGCA427070.15232484515346834No Hit
TTTGTCGCTAAGATTCGA413410.14745267575548585No Hit
CCTGATGATTCATGATAACTCG406940.14514499376390852No Hit
CCTTTGTCGCTAAGATTCGA400100.14270534232304466No Hit
TTGGGCCAACCTATGGTGTACCGGT390610.1393205042859397No Hit
CTGATGATTCATGATAACTCGA373200.13311080668572925No Hit
TACCCCGCATCTCCACCA366830.13083879211287797No Hit
TTTTTACTTACTCCGTGAATCGGAG361150.12881288272923666No Hit
TTATTTTACTTACTCCGTGAATCGGA360070.12842767460699497No Hit
CCAGATTGAGGTTCTGGTCC349460.12464336148015794No Hit
CGAGGGCACGTCTGCCTGGGTGTCACA343010.12234281297232578No Hit
TGTGCGTGCTAAGTACATG341110.12166513201653027No Hit
AGAGGGTGAGAGCCCCGTCGTGCCCGG340210.12134412524799555No Hit
TTTACGATAGGTGTCAAGTGGA338600.12076987980650569No Hit
TGTTTTTGGTCCCAAGGCT328170.11704976803337556No Hit
ACGTTATTTTACTTACTCCGTGAATCGGAG318610.11363996280316237No Hit
GCGGGTGCACCGCCGACCGACCT310730.11082937020754728No Hit
CCAGTTTCAGGTACTGGTC310590.11077943582133078No Hit
TTTATTTTACTTACTCCGTGAATCGGA302250.10780477309957573No Hit
ATCTGAAGGTCGCGTGT293090.10453763754426683No Hit
CTGATGATTCATGATAACT286750.10227632319703339No Hit
TGAAGGTCACGGCGAGACGAGCCGTTTA286070.10203378474969604No Hit
CCCCCTGTTTTTGGTCCCAAGGCT283130.10098516263914929No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)