FastQCFastQC Report
Thu 22 Jun 2023
DRR395062_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR395062_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57908762
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length14-135
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGGTGCACCGCCGACCGACCTTG26156114.516779343340132No Hit
CGTTCGAATTGTAGTCTG17214382.9726727710048437No Hit
CTGATGATTCATGATAACTCG8831781.5251198082942958No Hit
CTGATGATTCATGATAACTC6413071.1074438096259078No Hit
GCGTTCGAATTGTAGTCTG4475130.7727897895658692No Hit
TCGTTCGAATTGTAGTCTG4407610.7611300687105002No Hit
CCAGATTGAGGTTCTGGTC4359210.7527720934528008No Hit
TTTTTACTTACTCCGTGAATCGGA3806010.6572425084825678No Hit
TCTGATGATTCATGATAACTCG3653150.6308458122451314No Hit
CGGGTGCACCGCCGACCGACCTTG3090330.5336549933497111No Hit
ACGCATTTATTAGATA2953740.5100678892081996No Hit
TTTTACTTACTCCGTGAATCGGA2905630.5017599927278708No Hit
CGTTCGAATTGTAGTCT2862610.49433106513311403No Hit
TACTTACTCCGTGAATCGG2840310.4904801798387608No Hit
TACTTACTCCGTGAATCGGA2705410.46718491408951207No Hit
TTACTTACTCCGTGAATCGGA2651960.45795487736380897No Hit
TCTGATGATTCATGATAACTC2578580.44528321983467717No Hit
TTTACTTACTCCGTGAATCGGA2538900.4384310616068774No Hit
CCAGACTCAAGTTCTGGTC2508850.43324186415865706No Hit
CCAGATTTAGGCTCTGGTC2423620.41852388417490255No Hit
GCGGGTGCACCGCCGACCGACCTT2213320.3822081363093205No Hit
TGCGGGTGCACCGCCGACCGACCTTG2197490.37947452580664737No Hit
GCGTTCGAATTGTAGTCT1999120.34521891523082465No Hit
ATTTTACTTACTCCGTGAATCGGA1994460.3444142010841123No Hit
GTTCGAATTGTAGTCTG1920210.331592307222869No Hit
ACGTTATTTTACTTACTCCGTGAATCGGA1900060.32811269562281437No Hit
TTACTTACTCCGTGAATCGG1648030.2845907843790548No Hit
TACTTACTCCGTGAATCG1603800.27695290740285555No Hit
TTTTTACTTACTCCGTGAATCGGAG1600260.2763416009480569No Hit
AAATGCAGCCCCAATCGGGCGGT1576200.2721867892806964No Hit
TCGGGTGCACCGCCGACCGACCTTG1517460.2620432465815795No Hit
TCGAGGCAAACTCTGAATACTAGAT1490030.2573064849840858No Hit
AAGCCATCTCGTACTAAACGA1485620.2565449421971756No Hit
GTCGTTGTAGTATAGTGG1420790.2453497451732779No Hit
TTTGTCGCTAAGATTCGA1318480.2276822978878395No Hit
TTCGAATTGTAGTCTG1287220.22228415105817667No Hit
TGTGCTTGCTAAGTACATG1242300.21452712112892347No Hit
CGAGGCAAACTCTGAATACTAGAT1184050.20446819429501875No Hit
CCGACCCTCAGTAGGAGCTT1122220.19379105358874707No Hit
TACTTACTCCGTGAATCGGAGGCGGGG1121070.19359246533365712No Hit
ATTTTACTTACTCCGTGAATCGGAG1089660.18816841568811296No Hit
TGAATGCAGCCCCAATCGGGCGGTA1081700.186793839592012No Hit
CCTGATGATTCATGATAACTCG1060640.18315708424227753No Hit
TTACTTACTCCGTGAATCG1060010.18304829241557607No Hit
TTTACGATAGGTGTCAAGTGGA1046430.18070322415112242No Hit
AAATGCAGCCCCAATCGGGCGGTA1041220.17980353301284527No Hit
TTTTACTTACTCCGTGAATCGGAG968600.1672631164175121No Hit
AGGCTCCCAAAGGCACGT964930.16662936085561628No Hit
CTGTTTTTGGTCCCAAGGCT953780.1647039182084397No Hit
CTGATGATTCATGATAACTCGA931750.16089965798267283No Hit
CCAGTTTCAGGTACTGGTC928830.16039541650018352No Hit
GTCGTTGTAGTATAGTGGT901360.1556517474851215No Hit
TTGGGCCAACCTATGGTGTACCGGT872210.15061796693218893No Hit
TCGAATTGTAGTCTG867110.14973727119222477No Hit
GCGGGTGCACCGCCGACCGACCT859090.14835233396977127No Hit
CCTTTGTCGCTAAGATTCGA855440.14772203211665966No Hit
TTATTTTACTTACTCCGTGAATCGGA853510.14738874921898693No Hit
ACGTTATTTTACTTACTCCGTGAATCG841620.1453355193467959No Hit
TTACTTACTCCGTGAATCGGAGGCGGGG834420.14409218418449354No Hit
AATGCAGCCCCAATCGGGCGGT809860.13985102979752875No Hit
TTTGCTTGCTAAGTAAATG779260.13456685535774363No Hit
TGATGATTCATGATAACTCG776180.13403498420498092No Hit
CCTGATGATTCATGATAACTC766590.13237893084296984No Hit
TCCGTTGTAGTCTAGCTG744600.12858157803477133No Hit
CGCGGGTGCACCGCCGACCGACCTTG741330.12801689664855898No Hit
CTGATGATTCATGATAACT730730.12618643099294716No Hit
TTTACTTACTCCGTGAATCGG726140.12539380482697937No Hit
TTTTACTTACTCCGTGAATCGG713600.12322832941930274No Hit
TGCGTTCGAATTGTAGTCTG712580.1230521902713099No Hit
TTTTTACTTACTCCGTGAATCGG698250.12057760792744973No Hit
ACTTACTCCGTGAATCG697490.12044636699365115No Hit
CCTGCGGAAGGATCATTG696730.12031512605985256No Hit
AAGCCATCTCGTACTAAACGAA694940.12000601912366905No Hit
ATCCTGCCGTTCACGCCA685730.11841558622855727No Hit
ACGTTATTTTACTTACTCCGTGAATCGG679210.11728967716491676No Hit
TTTGGGCCAACCTATGGTGTACCGGT672750.1161741292276288No Hit
CGACCCTCAGTAGGAGCTT667770.11531415574036964No Hit
AATGCAGCCCCAATCGGGCGGTA659720.1139240379547399No Hit
TGAATGCAGCCCCAATCGGGCGGT649620.11217991501873238No Hit
ATGCAGCCCCAATCGGGCGGT649230.11211256769744103No Hit
TGAGGCAAACTCTGAATACTAGAT636630.10993673116341185No Hit
ACGTTATTTTACTTACTCCGTGAATC631720.1090888456568973No Hit
ATCTCTCAGGCGACGCCA624020.1077591677749906No Hit
TCCGTTGTAGTCTAGCTGG616320.10642948989308387No Hit
TATTTTACTTACTCCGTGAATCGGA614640.10613937835521332No Hit
TCGTTCGAATTGTAGTCT610970.10550562279331753No Hit
CATGATTGGAAATCATGTG599660.10355255047586755No Hit
TTTTGTCGCTAAGATTCGA596290.10297060054573434No Hit
ATTGATTCGAAATCAATTG584230.10088801414887785No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)