FastQCFastQC Report
Thu 22 Jun 2023
DRR395063_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR395063_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31590654
Total Bases734.1 Mbp
Sequences flagged as poor quality0
Sequence length14-134
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGGTGCACCGCCGACCGACCTTG12834994.062907339620129No Hit
CGTTCGAATTGTAGTCTG5175711.6383674741269998No Hit
CTGATGATTCATGATAACTCG4988721.5791759170291315No Hit
CTGATGATTCATGATAACTC2984150.9446306493053294No Hit
GCGTTCGAATTGTAGTCTG2007110.6353493029932207No Hit
CCAGATTGAGGTTCTGGTC1990800.6301863836057335No Hit
TCTGATGATTCATGATAACTCG1983020.6277236299064907No Hit
ACGCATTTATTAGATA1903650.6025991104837526No Hit
TCGAGGCAAACTCTGAATACTAGAT1816090.5748820521411174No Hit
GCGGGTGCACCGCCGACCGACCTT1707710.540574436983799No Hit
TACTTACTCCGTGAATCGGA1651230.5226957314653885No Hit
CGGGTGCACCGCCGACCGACCTTG1645290.5208154285124962No Hit
TTACTTACTCCGTGAATCGGA1561350.49424427870344184No Hit
AGGCTCCCAAAGGCACGT1520050.48117079184242273No Hit
TCGTTCGAATTGTAGTCTG1389250.43976614096055117No Hit
CCAGACTCAAGTTCTGGTC1379060.4365405034033167No Hit
CCAGATTTAGGCTCTGGTC1276290.40400872992372994No Hit
TTTACTTACTCCGTGAATCGGA1232680.3902040141365861No Hit
CGTTCGAATTGTAGTCT1219070.3858957779095045No Hit
CAGTGGCTGGTTGAGAT1217060.3852595137789803No Hit
TGCGGGTGCACCGCCGACCGACCTTG1180430.37366431223614427No Hit
CCGACCCTCAGTAGGAGCTT1178080.3729204213372727No Hit
TCTGATGATTCATGATAACTC1173020.3713186817848089No Hit
CGAGGCAAACTCTGAATACTAGAT1119670.35443077563383146No Hit
ACGTTATTTTACTTACTCCGTGAATCGGA1114090.35266443043565987No Hit
TTTTTACTTACTCCGTGAATCGGA1086970.34407961291336353No Hit
CGCGGGTGCACCGCCGACCGACCTTG997950.31590039256547203No Hit
TACTTACTCCGTGAATCGGAGGCGGGG852430.2698361357127966No Hit
TTTTACTTACTCCGTGAATCGGA834960.26430601911565366No Hit
TGTGCTTGCTAAGTACATG806100.2551704057788737No Hit
ATTTTACTTACTCCGTGAATCGGA722500.2287068827381668No Hit
GCGTTCGAATTGTAGTCT717260.2270481643083426No Hit
TCGCGGGTGCACCGCCGACCGACCTTG695760.22024235395696462No Hit
GCGGGTGCACCGCCGACCGACCT661180.20929607851739948No Hit
TCGGGTGCACCGCCGACCGACCTTG645920.20446553591451444No Hit
TTACTTACTCCGTGAATCGGAGGCGGGG645000.20417431054133925No Hit
CTGATGATTCATGATAACTCGA643800.2037944513589367No Hit
CGACCCTCAGTAGGAGCTT638620.20215472588823263No Hit
TTTGTCGCTAAGATTCGA629350.1992203137041734No Hit
CCTGATGATTCATGATAACTCG595000.18834684460790208No Hit
TACTTACTCCGTGAATCGG585450.1853237986146156No Hit
GTTCGAATTGTAGTCTG552880.1750137873055746No Hit
CTGTTTTTGGTCCCAAGGCT550490.1742572344339563No Hit
TTTGCTTGCTAAGTAAATG535300.1694488502833781No Hit
CCAGTTTCAGGTACTGGTC464100.1469105387941636No Hit
GTCGTTGTAGTATAGTGG464090.14690737330097692No Hit
TCAGTGGCTGGTTGAGAT453870.14367223926418238No Hit
CCAGATTGAGGTTCTGGTCC439790.13921522485732646No Hit
CCCCCTGTTTTTGGTCCCAAGGCT431520.13659736199193598No Hit
CGAGGGCACGTCTGCCTGGGTGTCACA431470.13658153452600252No Hit
GTCGTTGTAGTATAGTGGT427260.13524886189440713No Hit
ATTTTACTTACTCCGTGAATCGGAG424950.13451763296828234No Hit
CCTTTGTCGCTAAGATTCGA423390.1340238160311591No Hit
TTTACGATAGGTGTCAAGTGGA421330.13337172443470147No Hit
ATCCTGCCGTTCACGCCA400220.1266893683176043No Hit
AAGCCATCTCGTACTAAACGA398320.1260879246121337No Hit
TGATGATTCATGATAACTCG392850.12435639983901568No Hit
TTTTTACTTACTCCGTGAATCGGAG390620.12365049485838439No Hit
TTACTTACTCCGTGAATCGG384210.12162141372571773No Hit
AGGCTCCCAAAGGCACG382660.12113076228178118No Hit
CTGATGATTCATGATAACT378030.1196651389363449No Hit
TGAGGCAAACTCTGAATACTAGAT376050.1190383712853808No Hit
AAATGCAGCCCCAATCGGGCGGT368790.11674022323184571No Hit
TCCCAGTAAAACGCGGCGTGTTCGAATT368590.11667691336811198No Hit
TGCGTTCGAATTGTAGTCTG363260.11498970549960758No Hit
ACGTTATTTTACTTACTCCGTGAATCGGAG358640.11352724764735798No Hit
CCTGATGATTCATGATAACTC352250.11150449750106473No Hit
TTTACGATAGGTGTCAAGTGGAA349580.11065931082021917No Hit
ATCTCTCAGGCGACGCCA344010.10889613111523426No Hit
TCCCAGTAAAACGCGGCGTGTTCGAATTC343910.10886447618336739No Hit
TTCGAATTGTAGTCTG343060.10859540926249896No Hit
TCGACCCTCAGTAGGAGCTT336060.10637956403181775No Hit
TTATTTTACTTACTCCGTGAATCGGA318950.10096340518939556No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)