FastQCFastQC Report
Thu 22 Jun 2023
DRR395064_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR395064_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31116808
Total Bases686.2 Mbp
Sequences flagged as poor quality0
Sequence length14-134
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGATTCATGATAACTC8006972.573197739305394No Hit
CGTTCGAATTGTAGTCT3056360.9822215697702669No Hit
TTGGGCCAACCTATGGTGTACCGGT2945900.9467230700526865No Hit
TCTGATGATTCATGATAACTC2175640.6991848264127863No Hit
TGTGCTTGCTAAGTACATG2095860.673545949828787No Hit
CTGATGATTCATGATAACTCG1828340.5875731212533111No Hit
CCAGATTGAGGTTCTGGTC1821390.5853396016712255No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACCTT1733430.5570719207445699No Hit
CGTTCGAATTGTAGTCTG1567990.5039045135992097No Hit
AAAAGCCAGTGGCGCGAAGCTACCGTGCGCTGG1497360.48120616999018667No Hit
TTACGATAGGTGTCAAGTGGA1497310.48119010150398456No Hit
TGAGGCAAACTCTGAATACTAGAT1493530.4799753239471093No Hit
TCCGATGTCGTCCAGCGG1493230.4798789130298969No Hit
AGAATCCTTTGCAGACGACTTAAAT1288900.4142134373165783No Hit
TTTGCTTGCTAAGTAAATG1270620.40833879876110685No Hit
CCAGATTTAGGCTCTGGTC1160840.3730588304558745No Hit
TTTACGATAGGTGTCAAGTGGA1149070.3692763088039107No Hit
TGGCTCCCAAAGGCACG1143580.36751198901892507No Hit
CTGATGATTCATGATAACTCGACGGATCGC1105790.35536742714741176No Hit
GTTCGAATTGTAGTCT1087800.3495859858119123No Hit
GCGCTACACTGATGTATTCAACG1048420.33693044607917366No Hit
ATATGCTTGTCTCAAAGA1030560.33119078280779957No Hit
GCATTTATTAGCTGATGATTCATGATAACTC1005870.3232561643212247No Hit
CGAGGCAAACTCTGAATACTAGAT949470.3051309118853065No Hit
ATGGCTCATTAAATCAGTTATAGTTTGTTTGATG936120.3008406260693578No Hit
GGCTCCCAAAGGCACG925240.29734412347179057No Hit
CCAGACTCAAGTTCTGGTC918270.2951041764952241No Hit
GCGTCTGTAGTCCAACGG894090.28733345656791015No Hit
TCGCTAAGATTCGA865970.27829653992787434No Hit
ATTAAATCAGTTATAGTTTGTTTGATG864430.27780163055285106No Hit
TTTGTCGCTAAGATTCGA849940.27314498325149544No Hit
CGGGTGCACCGCCGACCGACCTTG755440.24277554432961118No Hit
CGACCCTCAGTAGGAGCT749340.24081518901296045No Hit
GTCGTTGTAGTATAGTGG731010.23492448197128704No Hit
CTGATGATTCATGATAACT710070.22819499994986633No Hit
ACGCATTTATTAGATA709560.22803110139060534No Hit
TCGCGCTACACTGATGTATTCAACG707910.22750084134593754No Hit
ACTTACTCCGTGAATC706150.22693523063162518No Hit
CCTGATGATTCATGATAACTC670790.21557159718953176No Hit
GGGCTCCCAAAGGCACG658630.21166374134519195No Hit
TACTTACTCCGTGAATC649720.20880033710398574No Hit
TTGTCGCTAAGATTCGA630980.20277786847545543No Hit
GCATTTATTAGCTGATGATTCATGATAACT595540.19138852545543875No Hit
AAGCCATCTCGTACTAAACGA594070.1909161119610983No Hit
CGACCCTCAGTAGGAGCTT592550.19042762998055585No Hit
TGATGATTCATGATAACTC592300.19034728754954558No Hit
AGCCCTTTGTCGCTAAGATTCGA591040.1899423616972538No Hit
CCTTTGTCGCTAAGATTCGA576260.18519251717592627No Hit
TCGAGGCAAACTCTGAATACTAGAT573970.18445658050787214No Hit
TGTCGCTAAGATTCGA567770.1824640882188173No Hit
TTTTGTCGCTAAGATTCGA559100.17967781271138095No Hit
TCTGATGATTCATGATAACTCG534060.1716307148213917No Hit
ATCTCTCAGGCGACGCCA529430.1701427729990814No Hit
TGGCTCCCAAAGGCACGT529390.17012991821011975No Hit
TCCCCGGCAACGGCG525440.16886050780015738No Hit
TTGAACCATCGAGTCTTTGAACGC519660.1670029907951998No Hit
ATCAGTTATAGTTTGTTTGATG493070.15845776983294688No Hit
GTCGCTAAGATTCGA488530.15699875128580026No Hit
TGAATGCAGCCCCAATCGGGCGGTA470750.15128479759234945No Hit
GGGGAGGTAGTGACAATAAATAACA469790.15097628265726998No Hit
TCAGTCTGACACACTGA469680.15094093198762545No Hit
TCATTAAATCAGTTATAGTTTGTTTGATG463080.1488198918089542No Hit
TTTTACTTACTCCGTGAATCGGA461530.14832176873669048No Hit
TGTGCGTGCTAAGTACATG457870.14714555554670003No Hit
TTTGGGCCAACCTATGGTGTACCGGT452120.14529767963346368No Hit
CTGATGATTCATGATAACTCGACGGATCGCA448070.14399613225109723No Hit
TCATTAAATCAGTTATAGTTTGTTTGATGGT437900.1407278021575992No Hit
GGCTCCCAAAGGCACGT430830.1384557182086286No Hit
AATCAGTTATAGTTTGTTTGATG419730.13488851427177234No Hit
CTTTGTCGCTAAGATTCGA412990.1327224823317353No Hit
TGGCTCATTAAATCAGTTATAGTTTGTTTGATG400610.12874392514810645No Hit
GTTCGAATTGTAGTCTG400560.1287278566619044No Hit
CCTGCGGAAGGATCATTG382370.12288214138159673No Hit
TAGAATCCTTTGCAGACGACTTAAAT380570.12230367587832275No Hit
GGGGCCAACCTATGGTGTACCGGT371550.11940492096747199No Hit
TCGTTCGAATTGTAGTCT370730.11914139779375828No Hit
AAATCAGTTATAGTTTGTTTGATG370700.11913175670203704No Hit
TCCGTTGTAGTCTAGCTGG370200.1189710718400165No Hit
AGAATCCTTTGCAGACGACTTAAATA364230.1170524945874911No Hit
CGCGCTACACTGATGTATTCAACG360340.11580236636097122No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACCTTG359190.11543279117832396No Hit
AAGCCAGTGGCGCGAAGCTACCGTGCGCTGG333720.10724750430699703No Hit
CCCCTTTGTCGCTAAGATTCGA327650.10529679008206753No Hit
CCAGTTTCAGGTACTGGTC326570.10494971078010315No Hit
AGAATCCTTTGCAGACGACTT326310.10486615465185246No Hit
TTGAACCATCGAGTCTTTGAACGCAA321500.10332036627921477No Hit
ATTGAGAGGACCTTTCCCT321050.10317574990339627No Hit
TCTGATGATTCATGATAACTCGACGGATCGC314470.10106113711920581No Hit
GGGCTCCCAAAGGCACGT312150.10031555935943046No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)