FastQCFastQC Report
Thu 22 Jun 2023
DRR395065_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR395065_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43879369
Total Bases970 Mbp
Sequences flagged as poor quality0
Sequence length14-134
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGATTCATGATAACTC11714902.6697968241065637No Hit
CGTTCGAATTGTAGTCT6142201.3997922349339162No Hit
CGTTCGAATTGTAGTCTG3727990.8495997287472388No Hit
TCTGATGATTCATGATAACTC3484750.7941659325137514No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACCTT3284870.7486137733657929No Hit
CTGATGATTCATGATAACTCG3210810.7317356819784715No Hit
TGAGGCAAACTCTGAATACTAGAT2709550.617499763955129No Hit
TTACGATAGGTGTCAAGTGGA2409980.5492284996167561No Hit
TGTGCTTGCTAAGTACATG2316410.5279041273360152No Hit
CGAGGCAAACTCTGAATACTAGAT2221020.5061649815429206No Hit
CCAGATTGAGGTTCTGGTC2132560.4860051656622501No Hit
TTGGGCCAACCTATGGTGTACCGGT1971870.4493843108819545No Hit
TGGCTCCCAAAGGCACG1880860.42864335628892025No Hit
GTTCGAATTGTAGTCT1733370.3950307489608613No Hit
AGAATCCTTTGCAGACGACTTAAAT1697040.3867512315411828No Hit
TACTTACTCCGTGAATC1675220.381778507343622No Hit
ACTTACTCCGTGAATC1550900.35344628588437543No Hit
GGCTCCCAAAGGCACG1491660.3399456359547923No Hit
GGGGAGGTAGTGACAATAAATAACA1483400.3380632023218018No Hit
TTTGTCGCTAAGATTCGA1480760.33746155283135454No Hit
TCGAGGCAAACTCTGAATACTAGAT1438710.3278784615157068No Hit
ACGCATTTATTAGATA1431920.32633103725807905No Hit
CGGGTGCACCGCCGACCGACCTTG1424290.3245921790716726No Hit
CTGATGATTCATGATAACTCGACGGATCGC1419540.323509665783936No Hit
TTTGCTTGCTAAGTAAATG1413780.32219697598659636No Hit
TTTACGATAGGTGTCAAGTGGA1406550.32054927681389406No Hit
CCAGATTTAGGCTCTGGTC1375060.3133727834600356No Hit
TCGCTAAGATTCGA1269050.289213365853096No Hit
ATGGCTCATTAAATCAGTTATAGTTTGTTTGATG1236820.281868228323885No Hit
CCAGACTCAAGTTCTGGTC1133630.25835148176355954No Hit
CTGATGATTCATGATAACT1126150.25664680820729213No Hit
CCTGATGATTCATGATAACTC1058380.24120219231046827No Hit
AAAAGCCAGTGGCGCGAAGCTACCGTGCGCTGG1025830.2337841275702939No Hit
GGGCTCCCAAAGGCACG1023520.2332576842661525No Hit
GCATTTATTAGCTGATGATTCATGATAACTC1016680.23169886513181173No Hit
TCTGATGATTCATGATAACTCG992090.22609486476434976No Hit
TTGTCGCTAAGATTCGA991980.22606979603558108No Hit
TGGCTCCCAAAGGCACGT967790.22055695468182324No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACCTTG928100.21151170154703No Hit
GTTCGAATTGTAGTCTG917820.2091689148948336No Hit
GCGCTACACTGATGTATTCAACG907120.20673041127824784No Hit
TACTTACTCCGTGAATCG888660.20252342279580182No Hit
GCGGGTGCACCGCCGACCGACCTTG884280.2015252315957415No Hit
TTTTGTCGCTAAGATTCGA870260.19833010816541138No Hit
TCGTTCGAATTGTAGTCT866050.19737065954617533No Hit
AAGCCATCTCGTACTAAACGA860250.19604885384746532No Hit
CCTGCGGAAGGATCATTG858660.19568649676799135No Hit
TGATGATTCATGATAACTC851270.19400233398980737No Hit
GTCGTTGTAGTATAGTGG845880.1927739662801441No Hit
GCGTCTGTAGTCCAACGG843670.19227031272942874No Hit
TAGAATCCTTTGCAGACGACTTAAAT829820.18911393187992287No Hit
CGACCCTCAGTAGGAGCTT828330.18877436455387497No Hit
CCTTTGTCGCTAAGATTCGA807160.18394977375358337No Hit
GGCTCCCAAAGGCACGT785360.17898160750670777No Hit
TCCGATGTCGTCCAGCGG773910.1763721807394268No Hit
TGGGAGGTAGTGACAATAAATAACA727600.16581824592783No Hit
TGTCGCTAAGATTCGA717520.1635210387824857No Hit
TCGTTCGAATTGTAGTCTG690510.15736552638211365No Hit
GTCGCTAAGATTCGA689090.15704191188346395No Hit
TGTGCGTGCTAAGTACATG672440.15324741793802915No Hit
TCGCGCTACACTGATGTATTCAACG668490.15234722267770076No Hit
TACTTACTCCGTGAATCGG662390.15095704771871263No Hit
TTTTACTTACTCCGTGAATCGGA609460.13889443123031234No Hit
AGAATCCTTTGCAGACGACTTAAATA603560.13754983577817628No Hit
ATTCAGCCCTTTGTCGCTAAGATTCGA602440.13729459053980472No Hit
ACTTACTCCGTGAATCG592160.13495180388760833No Hit
CTTTGTCGCTAAGATTCGA587890.13397868141631664No Hit
CGACCCTCAGTAGGAGCT585620.13346135401354564No Hit
TTGAACCATCGAGTCTTTGAACGCAA577300.13156524652849952No Hit
TCGGGTGCACCGCCGACCGACCTTG572710.13051919684624452No Hit
CGTTCGAATTGTAGTC570280.12996540583799188No Hit
TTACTTACTCCGTGAATC561580.12798269728992684No Hit
GGGCTCCCAAAGGCACGT548340.12496533393631983No Hit
TCCGTTGTAGTCTAGCTG542000.12352046356910922No Hit
ATCTCTCAGGCGACGCCA538610.12274789092796663No Hit
TCCCCGGCAACGGCG531870.12121186154705188No Hit
CTCTAGGGATAACAGGCTGA531620.12115488716348678No Hit
CTGATGATTCATGATAACTCGACGGATCGCA504920.11507002299873546No Hit
ACTTACTCCGTGAAT492790.11230562590815742No Hit
TACTTACTCCGTGAAT484020.11030696453269417No Hit
CCCCTTTGTCGCTAAGATTCGA480780.10956857652169065No Hit
TTACTTACTCCGTGAATCG461470.10516787513512328No Hit
TTCGCTAAGATTCGA445320.10148732995681865No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)