FastQCFastQC Report
Thu 22 Jun 2023
DRR395066_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR395066_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54780401
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length14-134
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGGTGCACCGCCGACCGACCTTG27973815.106536186180893No Hit
CGTTCGAATTGTAGTCTG20014423.653573109112509No Hit
TACTTACTCCGTGAATCGGA12014072.193132905325027No Hit
TTTTTACTTACTCCGTGAATCGGA8970561.6375491665349438No Hit
TACTTACTCCGTGAATCGG8794701.6054464442492855No Hit
GCGTTCGAATTGTAGTCTG8374441.5287292256221345No Hit
TTACTTACTCCGTGAATCGGA7643481.395294641965107No Hit
TTTTACTTACTCCGTGAATCGGA7189691.312456621118929No Hit
CGGGTGCACCGCCGACCGACCTTG5467520.9980795868945902No Hit
ACGCATTTATTAGATA5275490.9630250789876474No Hit
ACGTTATTTTACTTACTCCGTGAATCGGA4464520.8149849067369916No Hit
TTTACTTACTCCGTGAATCGGA4444900.8114033338310175No Hit
ATTTTACTTACTCCGTGAATCGGA4361930.796257406001829No Hit
CTGATGATTCATGATAACTCG4214730.7693864818550707No Hit
TTACTTACTCCGTGAATCGG4087550.7461701494299028No Hit
AGGCTCCCAAAGGCACGT3998440.7299033827810059No Hit
TCGTTCGAATTGTAGTCTG3936840.7186584851761125No Hit
TTTACGATAGGTGTCAAGTGGA3678520.6715029340511764No Hit
CTGATGATTCATGATAACTC3146310.574349574403444No Hit
CGAGGGCACGTCTGCCTGGGTGTCACA2582390.47140764814773806No Hit
CGTTCGAATTGTAGTCT2561390.46757416032788807No Hit
TCGGGTGCACCGCCGACCGACCTTG2530570.4619480605846606No Hit
CGAGGCAAACTCTGAATACTAGAT2413310.4405425947867742No Hit
TCGAGGCAAACTCTGAATACTAGAT2402560.43858021411708903No Hit
TGCGGGTGCACCGCCGACCGACCTTG2351930.42933785753046966No Hit
TGTGCTTGCTAAGTACATG2193470.40041145372411563No Hit
TTTACGATAGGTGTCAAGTGGAA2122060.38737576966623516No Hit
GCGGGTGCACCGCCGACCGACCTT2047070.3736865672085898No Hit
CTGTTTTTGGTCCCAAGGCT1855300.3386795215317975No Hit
GTTCGAATTGTAGTCTG1840370.33595409423892314No Hit
CCAGATTGAGGTTCTGGTC1819670.3321753705307853No Hit
TTTTACTTACTCCGTGAATCGG1815180.33135573432549353No Hit
TTATTTTACTTACTCCGTGAATCGGA1769430.32300420728939166No Hit
AGGCTCCCAAAGGCACG1748900.31925651657789067No Hit
GCGTTCGAATTGTAGTCT1706490.31151469665218406No Hit
TGAGGCAAACTCTGAATACTAGAT1643990.3001055067121542No Hit
TTACGATAGGTGTCAAGTGGA1592920.29078282942835704No Hit
ACTTACTCCGTGAATCGG1579340.28830384063818737No Hit
TCTGATGATTCATGATAACTCG1573000.28714649241067075No Hit
TTTACTTACTCCGTGAATCGG1561760.28509466369185577No Hit
TTTTTACTTACTCCGTGAATCGG1529060.2791253755152322No Hit
TCGCTAAGATTCGA1521260.27770150861071646No Hit
TACTTACTCCGTGAATCG1516200.27677782059317163No Hit
TATTTTACTTACTCCGTGAATCGGA1504020.2745543976576586No Hit
TTTGTCGCTAAGATTCGA1500000.2738205585607159No Hit
CCGACCCTCAGTAGGAGCTT1446680.26408715043907766No Hit
CTGATGATTCATGATAACTCGACGGATCGCA1434220.2618126143326333No Hit
TGGCTCCCAAAGGCACGT1375410.25107702296666284No Hit
TTTGCTTGCTAAGTAAATG1256290.22933201967616118No Hit
CCAGATTTAGGCTCTGGTC1205270.22001846974431605No Hit
CTGATGATTCATGATAACTCGACGGATCGC1170620.2136932148415635No Hit
TGCGTTCGAATTGTAGTCTG1165740.21280238529104598No Hit
TTCGAATTGTAGTCTG1159350.21163590971157734No Hit
ACTTACTCCGTGAATCGGA1150420.21000576465294588No Hit
TTGTCGCTAAGATTCGA1111320.2028681754264632No Hit
TCTGATGATTCATGATAACTC1105280.2017655913106587No Hit
GGCTCCCAAAGGCACGT1103040.20135668594320807No Hit
TCTGATGATTCATGATAACTCGACGGATCGCA1096930.20024132353467072No Hit
CGACCCTCAGTAGGAGCTT1060030.19350533779407714No Hit
TTTATTTTACTTACTCCGTGAATCGGA1028790.18780256829445263No Hit
CCAGACTCAAGTTCTGGTC1019800.18616147041347872No Hit
AAGCCATCTCGTACTAAACGA942870.1721181267000948No Hit
ACTTACTCCGTGAATCG940210.17163255157624713No Hit
TGTCGCTAAGATTCGA914200.16688450309080433No Hit
TTGGGCCAACCTATGGTGTACCGGT904860.16517951374616627No Hit
TTACGATAGGTGTCAAGTGGAA885030.1615596059619936No Hit
TTACTTACTCCGTGAATCG873750.159500475361617No Hit
TTTTGTCGCTAAGATTCGA869160.1586625844524212No Hit
TGAAGGTCACGGCGAGACGAGCCGTTTA838390.15304561206114572No Hit
TGAGGGCACGTCTGCCTGGGTGTCACA835730.15256003693729808No Hit
TACTTACTCCGTGAATCGGAGGCGGGG834130.1522679616748333No Hit
CGCGGGTGCACCGCCGACCGACCTTG827390.15103759463170047No Hit
GCGGGTGCACCGCCGACCGACCT801630.14633518290601777No Hit
TGAATGCAGCCCCAATCGGGCGGTA799510.14594818318325198No Hit
CGTTATTTTACTTACTCCGTGAATCGGA792500.14466852843957823No Hit
TCGAATTGTAGTCTG778110.14204167654778577No Hit
GTCGCTAAGATTCGA776560.14175872863727304No Hit
TTTGGGCCAACCTATGGTGTACCGGT770950.14073463974825595No Hit
ACGTTATTTTACTTACTCCGTGAATCGG751200.13712933572720654No Hit
TCCGTTGTAGTCTAGCTGG717440.13096654768920002No Hit
TGGCTCCCAAAGGCACG717260.13093368922217272No Hit
CCTTTGTCGCTAAGATTCGA715880.13068177430829686No Hit
GGGCTCCCAAAGGCACGT691860.1262969944305446No Hit
GTCGTTGTAGTATAGTGG685560.1251469480845896No Hit
AAAAGCCAGTGGCGCGAAGCTACCGTGCGCTGG680120.12415389219220939No Hit
ATTTTACTTACTCCGTGAATCGG671660.12260954424192697No Hit
CTGATGATTCATGATAACTCGA650940.11882716959300828No Hit
TACCTGGTTGATCCTGCCAG620520.11327408866539695No Hit
GCGGGTGCACCGCCGACCGACC611380.11160560872856698No Hit
CCCCCTGTTTTTGGTCCCAAGGCT595730.10874874756758351No Hit
TGTGCGTGCTAAGTACATG589140.10754576258030678No Hit
ACGTTATTTTACTTACTCCGTGAATCG588220.10737781930438954No Hit
TCGAGGGCACGTCTGCCTGGGTGTCACA581450.1061419758500855No Hit
GGCTCCCAAAGGCACG572530.10451365626184446No Hit
GGGTGCACCGCCGACCGACCTTG561100.10242714360561178No Hit
TTACTTACTCCGTGAATCGGAGGCGGGG560510.10231944085257792No Hit
CTACTTACTCCGTGAATCGGA551370.10065096091574795No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)