FastQCFastQC Report
Thu 22 Jun 2023
DRR395067_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR395067_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16153990
Total Bases352.3 Mbp
Sequences flagged as poor quality0
Sequence length14-134
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGGTGCACCGCCGACCGACCTTG8751665.41764604286619No Hit
CGTTCGAATTGTAGTCTG6161023.813930799759069No Hit
TACTTACTCCGTGAATCGGA3174511.9651553579022891No Hit
TTACTTACTCCGTGAATCGGA2254001.395320908332864No Hit
CGTTCGAATTGTAGTCT2020911.2510283836996308No Hit
CGGGTGCACCGCCGACCGACCTTG1992361.2333547315554856No Hit
TACTTACTCCGTGAATCGG1906711.18033377512305No Hit
TTTTTACTTACTCCGTGAATCGGA1789231.1076087084367392No Hit
ACGCATTTATTAGATA1627221.0073176967424147No Hit
TTTTACTTACTCCGTGAATCGGA1599790.9903373717576895No Hit
CTGATGATTCATGATAACTCG1401190.8673956093819546No Hit
CTGATGATTCATGATAACTC1384160.85685332230613No Hit
TCGTTCGAATTGTAGTCTG1338890.8288292861392139No Hit
TTTACTTACTCCGTGAATCGGA1276360.7901205832119494No Hit
GCGTTCGAATTGTAGTCTG1242090.768906010217909No Hit
TCGGGTGCACCGCCGACCGACCTTG967970.5992141879498502No Hit
GCGGGTGCACCGCCGACCGACCTT954860.5910985459319957No Hit
TTACTTACTCCGTGAATCGG935400.5790519865370723No Hit
TACTTACTCCGTGAATCG848900.5255048443140055No Hit
AGGCTCCCAAAGGCACGT839480.5196734676695974No Hit
TGGCTCCCAAAGGCACGT813870.5038197993189298No Hit
ATTTTACTTACTCCGTGAATCGGA793270.4910675319224538No Hit
CGAGGGCACGTCTGCCTGGGTGTCACA781120.48354617032695946No Hit
ACTTACTCCGTGAATCG752330.4657239480772243No Hit
TGCGGGTGCACCGCCGACCGACCTTG722360.4471712561416715No Hit
TGAGGCAAACTCTGAATACTAGAT715920.44318462497500616No Hit
GTTCGAATTGTAGTCTG705920.4369942039087557No Hit
TGTGCTTGCTAAGTACATG649380.4019935632001754No Hit
TCGAGGCAAACTCTGAATACTAGAT645700.3997154882477951No Hit
CGAGGCAAACTCTGAATACTAGAT640870.39672551487279617No Hit
GGCTCCCAAAGGCACGT623630.3860532289545803No Hit
TGGCTCCCAAAGGCACG611950.3788228171491997No Hit
TTTACGATAGGTGTCAAGTGGA605540.37485475724573314No Hit
ACTTACTCCGTGAATCGG595900.36888719133786757No Hit
TCTGATGATTCATGATAACTCG542760.3359912937918124No Hit
GCGTTCGAATTGTAGTCT541350.3351184444214711No Hit
AGGCTCCCAAAGGCACG539030.333682266734101No Hit
TCTGATGATTCATGATAACTC519460.32156761270744877No Hit
GGCTCCCAAAGGCACG482330.2985825792884606No Hit
TTACTTACTCCGTGAATCG479740.29697926023230176No Hit
CCGACCCTCAGTAGGAGCTT438620.2715242488078797No Hit
TTACGATAGGTGTCAAGTGGA426700.26414526689690904No Hit
ACTTACTCCGTGAATC425450.2633714642636278No Hit
ACTTACTCCGTGAATCGGA414040.2563081938270359No Hit
TTTGCTTGCTAAGTAAATG412070.2550886808769846No Hit
TGAGGGCACGTCTGCCTGGGTGTCACA410450.254085832664252No Hit
GGGCTCCCAAAGGCACGT388730.2406402381083559No Hit
TTTACTTACTCCGTGAATCGG380170.23534123767564546No Hit
CTGATGATTCATGATAACTCGACGGATCGC379810.23511838251726044No Hit
TTCGAATTGTAGTCTG371530.22999271387440504No Hit
TCGTTCGAATTGTAGTCT348930.2160023622646789No Hit
AAGCCATCTCGTACTAAACGA327870.20296533549915532No Hit
TCGCTAAGATTCGA321650.19911489359594753No Hit
TTTGTCGCTAAGATTCGA318110.19692348453849481No Hit
TTTTACTTACTCCGTGAATCGG313440.19403255790055585No Hit
CGACCCTCAGTAGGAGCTT311450.192800664108372No Hit
CCAGATTGAGGTTCTGGTC299880.18563834693472017No Hit
TTGGGCCAACCTATGGTGTACCGGT295390.18285884787597367No Hit
CTGTTTTTGGTCCCAAGGCT278470.1723846554318778No Hit
ACGTTATTTTACTTACTCCGTGAATCGGA278120.17216799069455904No Hit
GGGCTCCCAAAGGCACG276950.17144371142980774No Hit
CTGATGATTCATGATAACTCGACGGATCGCA273650.16940087247794508No Hit
TCGAATTGTAGTCTG263860.16334045025408583No Hit
TGTGCGTGCTAAGTACATG261370.16179903540858948No Hit
TACTTACTCCGTGAATC260220.16108713698597066No Hit
GTCGTTGTAGTATAGTGG259430.16059809372173686No Hit
TTTTTACTTACTCCGTGAATCGG254860.15776907129446038No Hit
TTATTTTACTTACTCCGTGAATCGGA243040.1504519935941523No Hit
CGGGTGCACCGCCGACCGACCTT238640.14772820832500205No Hit
TTTACTTACTCCGTGAATCG237420.14697297695491948No Hit
CGTTCGAATTGTAGTC234490.1451591835825081No Hit
GTTCGAATTGTAGTCT227760.14099303020492152No Hit
AAAAGCCAGTGGCGCGAAGCTACCGTGCGCTGG221900.1373654434600987No Hit
TTGTCGCTAAGATTCGA219890.13612116882578235No Hit
GCGGGTGCACCGCCGACCGACCT213160.13195501544819577No Hit
TATTTTACTTACTCCGTGAATCGGA209410.12963360754835182No Hit
CCAGATTTAGGCTCTGGTC203960.1262598280672453No Hit
TGAATGCAGCCCCAATCGGGCGGTA201670.12484222164307393No Hit
CTGATGATTCATGATAACTCGA198130.12265081258562127No Hit
TCTGATGATTCATGATAACTCGACGGATCGCA195180.12082463837107738No Hit
CCTTTGTCGCTAAGATTCGA192700.11928941394664723No Hit
TTTTGTCGCTAAGATTCGA189210.11712895699452582No Hit
TTTACGATAGGTGTCAAGTGGAA186110.11520992646398816No Hit
GGGTGCACCGCCGACCGACCTTG182560.11301232698546922No Hit
TCGAGGGCACGTCTGCCTGGGTGTCACA182260.11282661435348171No Hit
TTTGGGCCAACCTATGGTGTACCGGT181060.11208376382553165No Hit
GTCGCTAAGATTCGA179670.11122329529732282No Hit
CCGTTCGAATTGTAGTCTG176730.10940331150384518No Hit
TGTCGCTAAGATTCGA176520.10927331266145392No Hit
ACGTTATTTTACTTACTCCGTGAATCG175180.10844379623857635No Hit
TGCGTTCGAATTGTAGTCTG173670.10750904265757252No Hit
TCTGATGATTCATGATAACTCGACGGATCGC172380.10671047834002621No Hit
ATCGAGTCTTTGAACGC170860.10576953433795613No Hit
ACGCATTTATTAGAT167900.10393716970234598No Hit
CCTGATGATTCATGATAACTCG163760.10137433538091828No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)