FastQCFastQC Report
Thu 22 Jun 2023
DRR395080_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR395080_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16432081
Total Bases347.3 Mbp
Sequences flagged as poor quality0
Sequence length14-135
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTCGAATTGTAGTCTG9510065.787495813829057No Hit
CTGATGATTCATGATAACTC4046872.4627860585643413No Hit
CGTTCGAATTGTAGTCT3819192.3242278321291137No Hit
GCGGGTGCACCGCCGACCGACCTTG2733481.663502023876343No Hit
CTGATGATTCATGATAACTCG2436101.4825267718677873No Hit
TTTTACTTACTCCGTGAATCGGA2062461.2551423036437077No Hit
TACTTACTCCGTGAATCG1897861.1549723982008122No Hit
TACTTACTCCGTGAATCGG1608600.9789386992432668No Hit
CGGGTGCACCGCCGACCGACCTTG1266500.7707483915153533No Hit
TTACGATAGGTGTCAAGTGGA1240170.7547248580383702No Hit
TTACTTACTCCGTGAATCG1053240.6409656817051961No Hit
TTACTTACTCCGTGAATCGG953220.5800969457246468No Hit
TTTTACTTACTCCGTGAATCGG883210.5374912648008491No Hit
TTACTTACTCCGTGAATCGGA827040.5033081324270492No Hit
GTTCGAATTGTAGTCTG800430.4871142005689967No Hit
GGCTCCCAAAGGCACG753250.45840207335881555No Hit
TTTTTACTTACTCCGTGAATCGGA705550.42937349201236286No Hit
GGCTCCCAAAGGCACGT679120.413289101970712No Hit
CGTTCGAATTGTAGTC676610.4117616021975549No Hit
ATTTTACTTACTCCGTGAATCGGA638130.3883439961134564No Hit
GCGTTCGAATTGTAGTCTG622370.3787530015218401No Hit
CTGATGATTCATGATAACT621440.3781870354704313No Hit
AGGCTCCCAAAGGCACGT605630.36856561259648124No Hit
TTTTACTTACTCCGTGAATCG604260.36773187766053494No Hit
AGGCTCCCAAAGGCACG596530.3630276652117282No Hit
TCTGATGATTCATGATAACTC586650.3570150366225678No Hit
TACTTACTCCGTGAATCGGA570280.34705281698647905No Hit
TTTACTTACTCCGTGAATCGGA553960.33712102563272417No Hit
TTTACGATAGGTGTCAAGTGGA516820.3145188975151717No Hit
CCTGCGGAAGGATCATTG501140.30497658817528955No Hit
GCGGGTGCACCGCCGACCGACCTT491850.29932301331766803No Hit
TTACGATAGGTGTCAAGTGGAA472160.28734035573461447No Hit
ACTTACTCCGTGAATCG459760.2797941417158301No Hit
TATTTTACTTACTCCGTGAATCGGA444770.27067174267215455No Hit
CGAGGCAAACTCTGAATACTAGAT438610.26692297828862943No Hit
TACGATAGGTGTCAAGTGGA433100.26356978157544375No Hit
TCGTTCGAATTGTAGTCTG426060.25928547942284363No Hit
TACCCCGCATCTCCACCA418030.2543986972800341No Hit
TTTACTTACTCCGTGAATCGG414970.2525364864012051No Hit
TCGCTAAGATTCGA408340.24850169616374213No Hit
TTTACTTACTCCGTGAATCG392700.23898372944972704No Hit
TCTGATGATTCATGATAACTCG382620.23284938773123134No Hit
GTCGTTGTAGTATAGTGG374650.2279991195272224No Hit
AGAGAGGGTGAGAGCCCCGTCGTGCCCGG355390.2162781451722396No Hit
TACTTACTCCGTGAATC350680.21341180097639492No Hit
GCGTTCGAATTGTAGTCT348200.21190255817263803No Hit
TTCGAATTGTAGTCTG348080.21182953029503687No Hit
TTGTCGCTAAGATTCGA345140.210040347293809No Hit
CTGATGATTCATGATAACTCGACGGATCGC345020.20996731941620786No Hit
CGTTCGAATTGTAGT332400.20228722095515472No Hit
CTGATGATTCATGATAACTCGA310030.18867360743900907No Hit
GTCGCTAAGATTCGA305740.18606286081476836No Hit
TTACTTACTCCGTGAATC303210.18452318972867768No Hit
TTATTTTACTTACTCCGTGAATCGGA301300.18336082934352624No Hit
ACTTACTCCGTGAATCGG294890.17945992354833207No Hit
ATAGTTTGTTTGATGGT283510.1725344464891574No Hit
AGCCCTTTGTCGCTAAGATTCGA267900.16303473674454258No Hit
CTGTTTTTGGTCCCAAGGCT266020.1618906333287914No Hit
CGAGGGCACGTCTGCCTGGGTGTCACA264940.16123338243038116No Hit
ATATGCTTGTCTCAAAGA261220.15896951822474586No Hit
TTTGTCGCTAAGATTCGA259890.15816012591466658No Hit
TCGAGGCAAACTCTGAATACTAGAT259300.15780107218312764No Hit
TCGAATTGTAGTCTG258700.15743593279512194No Hit
TTTTACTTACTCCGTGAATC252550.15369325406806356No Hit
ATTCAGCCCTTTGTCGCTAAGATTCGA245450.14937243797666283No Hit
ACGTTATTTTACTTACTCCGTGAATCGGA245070.1491411830309259No Hit
TGGCTCCCAAAGGCACG240490.1463539523691491No Hit
TCGGGTGCACCGCCGACCGACCTTG238780.14531330511333287No Hit
TGTCGCTAAGATTCGA234560.1427451580843595No Hit
ATTTTACTTACTCCGTGAATCGG234290.14258084535975693No Hit
CTGATGATTCATGATAAC229540.1396901585380452No Hit
TCCCCCGCATCTCCACCA228670.13916070642543693No Hit
ACGTTATTTTACTTACTCCGTGAATCG227520.13846085593175933No Hit
TCCGTTGTAGTCTAGCTG225540.13725589595134055No Hit
TTTTTACTTACTCCGTGAATCGG225480.13721938201253997No Hit
GTTCGAATTGTAGTCT225400.1371706967608059No Hit
TGATGATTCATGATAACTC222670.13550931254537998No Hit
CTGATGATTCATGATA217750.13251516956373327No Hit
CGGGTGCACCGCCGACCGACCTT207760.1264355987534385No Hit
TGGCTCCCAAAGGCACGT207290.12614957289950068No Hit
GCGTCTGTAGTCCAACGG204720.12458555918754294No Hit
CTGATGATTCATGATAACTCGACGGATCGCA203190.12365445374812843No Hit
TTGGGCCAACCTATGGTGTACCGGT200120.12178615721283262No Hit
AGTTTGTTTGATGGT192030.11686286113122252No Hit
TTTACGATAGGTGTCAAGTGGAA188630.11479373793252358No Hit
TATTTTACTTACTCCGTGAATCGG183270.11153182606633938No Hit
ACTTACTCCGTGAATCGGA182130.11083806122912855No Hit
CCTGATGATTCATGATAACTC181970.11074069072566037No Hit
ACGCATTTATTAGATA180930.11010778245311717No Hit
AAGCCATCTCGTACTAAACGA180600.10990695578971404No Hit
CCTTTGTCGCTAAGATTCGA177220.10785000390394862No Hit
CGTTCGAATTGTAG176690.10752746411121027No Hit
TTACGATAGGTGTCAAGTGG170760.10391866982642066No Hit
ACGTTATTTTACTTACTCCGTGAATC168630.10262242499900043No Hit
GTGGACGTGCCGGAGTGG168300.1024215983355973No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)