FastQCFastQC Report
Thu 22 Jun 2023
DRR395081_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR395081_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13810694
Total Bases305 Mbp
Sequences flagged as poor quality0
Sequence length14-136
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTCGAATTGTAGTCTG4141082.9984590202346095No Hit
CTGATGATTCATGATAACTC3489812.526889669700885No Hit
GCGGGTGCACCGCCGACCGACCTTG3160452.288407809194817No Hit
CTGATGATTCATGATAACTCG2959712.1430566776731133No Hit
TTTTACTTACTCCGTGAATCGGA2120831.5356433210380305No Hit
CGGGTGCACCGCCGACCGACCTTG1844591.33562440815791No Hit
TTACTTACTCCGTGAATCGG1169970.8471478696146624No Hit
TTACTTACTCCGTGAATCG1143670.828104655711002No Hit
TACTTACTCCGTGAATCG1123120.8132248821094725No Hit
TTTTACTTACTCCGTGAATCGG1031700.7470298016884597No Hit
CGTTCGAATTGTAGTCT961680.6963299599571173No Hit
TACTTACTCCGTGAATCGG913970.6617842665980436No Hit
TTACGATAGGTGTCAAGTGGA718080.5199449064616158No Hit
TTTTTACTTACTCCGTGAATCGGA710010.5141016085071467No Hit
TTTTACTTACTCCGTGAATCG682980.49452981870425916No Hit
GCGGGTGCACCGCCGACCGACCTT665390.4817933117626095No Hit
GGCTCCCAAAGGCACG621600.45008599857472764No Hit
ATTTTACTTACTCCGTGAATCGGA597010.4322809556130923No Hit
TACCCCGCATCTCCACCA565070.40915394983047193No Hit
GGCTCCCAAAGGCACGT539730.3908058494381238No Hit
TTACTTACTCCGTGAATCGGA528300.3825296541940615No Hit
TTTACTTACTCCGTGAATCGG527140.3816897253678924No Hit
CCTGCGGAAGGATCATTG487260.3528135515854598No Hit
TTTACTTACTCCGTGAATCGGA477000.34538452593330937No Hit
TTTACTTACTCCGTGAATCG459710.33286524196394474No Hit
CGAGGCAAACTCTGAATACTAGAT450890.3264788865787628No Hit
TCTGATGATTCATGATAACTC449400.32540001248308015No Hit
TCTGATGATTCATGATAACTCG435230.31513984742548057No Hit
CTGATGATTCATGATAACTCGACGGATCGC425900.308384212987414No Hit
AGAGAGGGTGAGAGCCCCGTCGTGCCCGG415510.30086105738060664No Hit
TCGCTAAGATTCGA413730.29957220107838173No Hit
TATTTTACTTACTCCGTGAATCGGA409500.29650935716916177No Hit
TTGTCGCTAAGATTCGA399700.2894134067411819No Hit
CTGTTTTTGGTCCCAAGGCT371070.2686830944194405No Hit
AGGCTCCCAAAGGCACGT368590.266887384515217No Hit
GTTCGAATTGTAGTCTG366660.2654899167268495No Hit
CTGATGATTCATGATAACTCGA338780.24530266183582083No Hit
TCGGGTGCACCGCCGACCGACCTTG333960.24181261274777358No Hit
AGGCTCCCAAAGGCACG333930.24179089045054505No Hit
ACTTACTCCGTGAATCG332870.24102336928180437No Hit
CGAGGGCACGTCTGCCTGGGTGTCACA330830.2395462530702657No Hit
ATTCAGCCCTTTGTCGCTAAGATTCGA325540.23571588799230508No Hit
GCGTTCGAATTGTAGTCTG318370.2305242589546912No Hit
GTCGTTGTAGTATAGTGG311960.2258829281135329No Hit
GTCGCTAAGATTCGA307370.22255941663757084No Hit
CTGATGATTCATGATAACTCGACGGATCGCA304400.22040890921194836No Hit
TTTGTCGCTAAGATTCGA292390.21171274955480152No Hit
TTATTTTACTTACTCCGTGAATCGGA282020.20420407547947989No Hit
TGTCGCTAAGATTCGA282000.20418959394799419No Hit
CGGGTGCACCGCCGACCGACCTT278590.2017204928296869No Hit
TTGGGCCAACCTATGGTGTACCGGT277770.2011267500387743No Hit
TCGAGGCAAACTCTGAATACTAGAT277650.20103986084986028No Hit
TACTTACTCCGTGAATCGGA272630.19740499644695628No Hit
TCCCCCGCATCTCCACCA268240.19422630028585094No Hit
TACGATAGGTGTCAAGTGGA264720.19167755074437243No Hit
TACTTACTCCGTGAATC264190.1912937901600021No Hit
TTTACGATAGGTGTCAAGTGGA247970.179549268125121No Hit
ACGTTATTTTACTTACTCCGTGAATCG245350.1776521875004978No Hit
ATTTTACTTACTCCGTGAATCGG238940.1730108566593395No Hit
TTTTACTTACTCCGTGAATC237910.17226505778782733No Hit
CTGATGATTCATGATAACT234210.16958597446297774No Hit
ATAGTTTGTTTGATGGT223290.1616790582718001No Hit
TTTTTACTTACTCCGTGAATCGG219270.15876827044317976No Hit
TCGTTCGAATTGTAGTCTG210050.15209228442828435No Hit
ATAACTCGACGGATCGC201460.14587246665518763No Hit
TTACTTACTCCGTGAATC200790.1453873353504176No Hit
TGAGGCAAACTCTGAATACTAGAT197940.14332371711370911No Hit
TGATGATTCATGATAACTC197420.14294719729508162No Hit
TATTTTACTTACTCCGTGAATCGG189800.13742973379904008No Hit
ACGTTATTTTACTTACTCCGTGAATCGGA184790.1338021101618789No Hit
CCTTTGTCGCTAAGATTCGA183720.13302734822739537No Hit
ACGTTATTTTACTTACTCCGTGAATC182390.1320643263835981No Hit
TCAGCCCTTTGTCGCTAAGATTCGA182210.13193399260022703No Hit
TGATGATTCATGATAACTCG180270.13052928404611672No Hit
AGCCCTTTGTCGCTAAGATTCGA172980.1252507658195888No Hit
TTCGAATTGTAGTCTG169470.12270925704385312No Hit
TGGCTCCCAAAGGCACG169070.12241962641413966No Hit
TTTTGTCGCTAAGATTCGA165990.12018947056534596No Hit
ACTTACTCCGTGAATCGG165250.11965365390037605No Hit
ACTTACTCCGTGAATC165030.11949435705403363No Hit
TGAGGGCACGTCTGCCTGGGTGTCACA163770.11858202057043621No Hit
GCCCCACGGTGGGCGCCA163030.11804620390546632No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACCTT161300.11679355143195556No Hit
CTTTGTCGCTAAGATTCGA159540.11551917666121629No Hit
TTTCAGCCCTTTGTCGCTAAGATTCGA157700.11418687576453435No Hit
CGACCCTCAGTAGGAGCTT156030.11297766788548062No Hit
TGTGCTTGCTAAGTACATG154590.11193499761851214No Hit
TTACGATAGGTGTCAAGTGGAA154530.11189155302405512No Hit
ATAACTCGACGGATCGCA151860.10995826857071775No Hit
TGGCTCCCAAAGGCACGT150170.10873457916017834No Hit
ATTTTACTTACTCCGTGAATCG149700.10839426317026502No Hit
ACGCATTTATTAGATA148630.1076195012357815No Hit
TTTTACTTACTCCGTGA147760.1069895546161547No Hit
TTTGCTTGCTAAGTAAATG146970.1064175341224706No Hit
CTGATGATTCATGAT146080.10577310597135814No Hit
TCCGTTGTAGTCTAGCTGG145530.10537486385550211No Hit
ACATAGTAAGGATTGACAGA143360.10380361768930657No Hit
TCGAATTGTAGTCTG140960.10206583391102576No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACCTTG138890.10056699540225855No Hit
CCTGATGATTCATGATAACTC138790.10049458774483021No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)