FastQCFastQC Report
Thu 22 Jun 2023
DRR395082_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR395082_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11311384
Total Bases288.5 Mbp
Sequences flagged as poor quality0
Sequence length14-136
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGGTGCACCGCCGACCGACCTTG4668224.127010452478671No Hit
CTGATGATTCATGATAACTCG1106640.9783418191796865No Hit
CGTTCGAATTGTAGTCTG1044910.9237684796131048No Hit
TTTACGATAGGTGTCAAGTGGA871750.7706837642502457No Hit
CGGGTGCACCGCCGACCGACCTTG862820.762789062770745No Hit
TTACGATAGGTGTCAAGTGGA797600.7051303359518164No Hit
TTACGATAGGTGTCAAGTGGAA762800.6743648699398765No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACCTTG734990.6497790190837832No Hit
CGAGGGCACGTCTGCCTGGGTGTCACA719690.636252822819913No Hit
TTTACGATAGGTGTCAAGTGGAA666150.5889199765475206No Hit
TACCCCGCATCTCCACCA665600.5884337407341136No Hit
CTGATGATTCATGATAACTCGACGGATCGC661620.5849151615752768No Hit
AAAAGCCAGTGGCGCGAAGCTACCGTGCGCTGG657480.5812551319979943No Hit
TCGAGGCAAACTCTGAATACTAGAT565100.499585196647908No Hit
GCGGGTGCACCGCCGACCGACCTT563220.497923154231171No Hit
CTGATGATTCATGATAACTC515240.4555057099997666No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACCTT513920.45433874404758956No Hit
CGAGGCAAACTCTGAATACTAGAT508640.44967088023888147No Hit
CTGATGATTCATGATAACTCGACGGATCGCA423170.3741098348354189No Hit
TCGCGCTACACTGATGTATTCAACG404410.3575247732726605No Hit
CGTTCGAATTGTAGTCT396480.3505141369084455No Hit
TTTTTACTTACTCCGTGAATCGGA382720.3383494009221153No Hit
TCCCCCGCATCTCCACCA380470.3363602544127226No Hit
ACGTTATTTTACTTACTCCGTGAATCGGA379550.33554691450665985No Hit
TGAGGGCACGTCTGCCTGGGTGTCACA379170.3352109697628513No Hit
TCGGGTGCACCGCCGACCGACCTTG378340.3344771957171642No Hit
ATTTTACTTACTCCGTGAATCGGA361770.3198282367568814No Hit
ACGTTATTTTACTTACTCCGTGAATCG348140.30777842923553833No Hit
TCTGATGATTCATGATAACTCG343100.30332274105449875No Hit
TGAGGCAAACTCTGAATACTAGAT326030.2882317495365731No Hit
TCGGATAACCGTAGTAATTCTAGAGCTAATACGTGC315440.27886949996569826No Hit
GCGTTCGAATTGTAGTCTG312610.2763675956894399No Hit
TTTTACTTACTCCGTGAATCGGA312150.27596092573640857No Hit
ATCAGGGCTCAAGTGATTCACCGAAGCGACGAGACCT308160.2724335059264189No Hit
TCAGGGCTCAAGTGATTCACCGAAGCGACGAGACCT297880.2633453165412827No Hit
TCTGATGATTCATGATAACTCGACGGATCGCA289300.25576003785213197No Hit
CTGTTTTTGGTCCCAAGGCT267330.23633712726930672No Hit
CTGATGATTCATGATAACTCGA266820.23588625406051109No Hit
TGCGGGTGCACCGCCGACCGACCTTG263290.2327655042035528No Hit
ACGTTATTTTACTTACTCCGTGAATC246120.2175861061740986No Hit
TCTGATGATTCATGATAACTCGACGGATCGC242970.21480130106094888No Hit
TATTTTACTTACTCCGTGAATCGGA235930.20857748264933806No Hit
CCTGCGGAAGGATCATTG225760.19958654042688323No Hit
AGAGAGGGTGAGAGCCCCGTCGTGCCCGG223750.19780956954515908No Hit
ACGTTATTTTACTTACTCCGTGAATCGG220400.19484795140895225No Hit
TTATTTTACTTACTCCGTGAATCGGA220200.19467113838589511No Hit
TCCCAGTAAAACGCGGCGTGTTCGAATT216790.19165647634277114No Hit
TAGAATCCTTTGCAGACGACTTAAAT205050.18127755188931788No Hit
TCGTTCGAATTGTAGTCTG187140.16544394567455228No Hit
GCGCTACACTGATGTATTCAACG183730.1624292836314283No Hit
TACTTACTCCGTGAATCG183720.16242044298027544No Hit
TCGAGGGCACGTCTGCCTGGGTGTCACA180610.15967100047173716No Hit
ACGCATTTATTAGATA173270.15318196252554064No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACCT172180.1522183315498793No Hit
TCTGATGATTCATGATAACTC166290.14701118802084695No Hit
AGAACATGAAACCGTAAGCTCCCAAGCAGTGGGAGGAGCC160110.14154766560838178No Hit
TTTTACTTACTCCGTGAATCG158200.13985910123818623No Hit
GCGGGTGCACCGCCGACCGACCT156130.138029086449545No Hit
GTGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGA148240.1310538126899414No Hit
GTGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGAG147220.13015206627235004No Hit
TAGAATCCTTTGCAGACGACTTAAATA147200.13013438497004434No Hit
CCCCCTGTTTTTGGTCCCAAGGCT145970.129046984878243No Hit
CGCGCTACACTGATGTATTCAACG144890.12809219455373455No Hit
AGGCTCCCAAAGGCACGT139270.12312374860582931No Hit
TTTTACTTACTCCGTGAATCGG138840.12274360060625648No Hit
ATGGCTCATTAAATCAGTTATAGTTTGTTTGATG125110.11060538657338484No Hit
AGAGGGTGAGAGCCCCGTCGTGCCCGG124200.1098008873184749No Hit
ACGTTATTTTACTTACTCCGTGAAT124100.10971248080694634No Hit
TTGGGCCAACCTATGGTGTACCGGT124100.10971248080694634No Hit
TTTGGGCCAACCTATGGTGTACCGGT123070.10880189373820215No Hit
TTTTTACTTACTCCGTGAATCGG121990.10784710341369366No Hit
AAAAGCCAGTGGCGCGAAGCTACCGTGCGCTG121120.10707796676339518No Hit
ATTCAGCCCTTTGTCGCTAAGATTCGA120530.10655636834537666No Hit
ATCTCTCAGGCGACGCCA117770.10411634862718833No Hit
ACTTACTCCGTGAATCG117750.10409866732488261No Hit
TCGGCGTTCGAATTGTAGTCTG115510.10211836146664281No Hit
TTACTTACTCCGTGAATCG114660.10136690611865004No Hit
ATCTGAAGGTCGCGTGT113930.10072153858449151No Hit
TACTTACTCCGTGAATCGGA113690.10050936295682297No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)