FastQCFastQC Report
Thu 22 Jun 2023
DRR395083_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR395083_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24414186
Total Bases624.7 Mbp
Sequences flagged as poor quality0
Sequence length14-135
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGGTGCACCGCCGACCGACCTTG8957613.6690184960497967No Hit
CTGATGATTCATGATAACTCG3661951.4999271325286045No Hit
TACCCCGCATCTCCACCA2951391.2088832287916542No Hit
TCGAGGCAAACTCTGAATACTAGAT2015100.8253807847617775No Hit
CGTTCGAATTGTAGTCTG1907710.7813940632712473No Hit
CGGGTGCACCGCCGACCGACCTTG1858000.7610329502691591No Hit
CGAGGGCACGTCTGCCTGGGTGTCACA1790000.7331802911635064No Hit
CGAGGCAAACTCTGAATACTAGAT1758520.7202861483893012No Hit
TTACGATAGGTGTCAAGTGGA1703500.697750070389404No Hit
TTTACGATAGGTGTCAAGTGGA1618060.6627540234190074No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACCTTG1584110.6488481737625822No Hit
TTTTTACTTACTCCGTGAATCGGA1575190.6451945602446054No Hit
CTGATGATTCATGATAACTCGACGGATCGC1488200.6095636364857709No Hit
ATTTTACTTACTCCGTGAATCGGA1406620.5761486375175482No Hit
TCCCCCGCATCTCCACCA1365800.5594288500955961No Hit
AAAAGCCAGTGGCGCGAAGCTACCGTGCGCTGG1328700.5442327669658943No Hit
ACGTTATTTTACTTACTCCGTGAATCGGA1315390.5387810185438908No Hit
TTACGATAGGTGTCAAGTGGAA1214250.4973542841035126No Hit
TCGCGCTACACTGATGTATTCAACG1157850.4742529609629418No Hit
TCTGATGATTCATGATAACTCG1107170.45349453797067No Hit
TTTTACTTACTCCGTGAATCGGA1056800.4328630903360857No Hit
CTGATGATTCATGATAACTCGACGGATCGCA1039660.42584258184974916No Hit
CTGTTTTTGGTCCCAAGGCT1033960.42350787366001064No Hit
GCGTTCGAATTGTAGTCTG1006380.4122111628051003No Hit
ATCAGGGCTCAAGTGATTCACCGAAGCGACGAGACCT958210.3924808306121695No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACCTT937940.38417828061111686No Hit
TTTACGATAGGTGTCAAGTGGAA930530.3811431599644567No Hit
TGAGGCAAACTCTGAATACTAGAT925760.3791893778477808No Hit
CTGATGATTCATGATAACTC912330.3736884776744144No Hit
TGAGGGCACGTCTGCCTGGGTGTCACA860480.35245082510635417No Hit
TATTTTACTTACTCCGTGAATCGGA816740.33453501173457106No Hit
TCGGGTGCACCGCCGACCGACCTTG792220.3244916705394151No Hit
GCGGGTGCACCGCCGACCGACCTT756030.3096683215242155No Hit
TTATTTTACTTACTCCGTGAATCGGA749870.30714519828758574No Hit
TCAGGGCTCAAGTGATTCACCGAAGCGACGAGACCT743840.3046753227815992No Hit
ACGTTATTTTACTTACTCCGTGAATCG719400.29466474942068516No Hit
CTGATGATTCATGATAACTCGA719210.2945869258143606No Hit
TCTGATGATTCATGATAACTCGACGGATCGCA704510.2885658362724033No Hit
TCCCAGTAAAACGCGGCGTGTTCGAATT609800.2497728165092213No Hit
ACGTTATTTTACTTACTCCGTGAATCGG589760.24156447403161424No Hit
GTGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGA537420.2201261184788221No Hit
TCTGATGATTCATGATAACTCGACGGATCGC507530.20788323641017561No Hit
GCGCTACACTGATGTATTCAACG500200.20488088359775747No Hit
TGCGGGTGCACCGCCGACCGACCTTG495480.20294758137748276No Hit
GTGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGAG490680.20098151132296607No Hit
CCCCCTGTTTTTGGTCCCAAGGCT485400.19881883426299773No Hit
TTTTACTTACTCCGTGAATCGG484280.19836008458361054No Hit
TTTTACTTACTCCGTGAATCG481920.19739343347347318No Hit
CCTGCGGAAGGATCATTG480050.19662748534806773No Hit
TCGAGGGCACGTCTGCCTGGGTGTCACA437410.17916222969711135No Hit
TCGGATAACCGTAGTAATTCTAGAGCTAATACGTGC433490.17755660581925606No Hit
ACGTTATTTTACTTACTCCGTGAATC406910.16666949289237004No Hit
TTTTTACTTACTCCGTGAATCGG402940.16504338911811353No Hit
AGAACATGAAACCGTAAGCTCCCAAGCAGTGGGAGGAGCC393810.16130376003525163No Hit
TTTGGGCCAACCTATGGTGTACCGGT380690.15592983521957274No Hit
TCGTTCGAATTGTAGTCTG379760.15554890914651015No Hit
TAGAATCCTTTGCAGACGACTTAAATA375000.15359922300911447No Hit
CGCGCTACACTGATGTATTCAACG371980.15236223726648104No Hit
TAGAATCCTTTGCAGACGACTTAAAT353350.14473142786738824No Hit
AGAGAGGGTGAGAGCCCCGTCGTGCCCGG352150.14423991035375908No Hit
ATCTCTCAGGCGACGCCA330320.1352983875849885No Hit
ATCAGGGCTCAAGTGATTCACCGAAGCGACGAGACCTT326790.1338525068990627No Hit
ATTTTACTTACTCCGTGAATCGG325930.1335002526809618No Hit
TCGGCGTTCGAATTGTAGTCTG321130.13153418262644515No Hit
TTGGGCCAACCTATGGTGTACCGGT317060.1298671190593862No Hit
CCTGTTTTTGGTCCCAAGGCT301120.1233381280866788No Hit
ATGGCTCATTAAATCAGTTATAGTTTGTTTGATG296030.12125327463303508No Hit
TTTGCTTGCTAAGTAAATG293400.12017603208233116No Hit
ACGTTATTTTACTTACTCCGTGAATCGGAG292180.1196763226101415No Hit
AAGCCATCTCGTACTAAACGA289530.1185908881008771No Hit
TTGGGCCAACCTATGGTGTACCGGTT286260.1172515028762376No Hit
ATCCGGTCCGCCGATCGATTCGGGGCGTGGACCG284780.11664529794276164No Hit
CGTTATTTTACTTACTCCGTGAATCGGA272120.11145978817397394No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACCTTGG268190.10985006831683841No Hit
TTTATTTTACTTACTCCGTGAATCGGA267230.10945685430593508No Hit
AGAGGGTGAGAGCCCCGTCGTGCCCGG262800.10764233548478741No Hit
AGGCTCCCAAAGGCACGT262070.10734332899732967No Hit
CGTTCGAATTGTAGTCT261000.10690505921434366No Hit
CCTGATGATTCATGATAACTCG260860.10684771550442025No Hit
TACTTACTCCGTGAATCG259970.10648317334847862No Hit
TTTCGGCGTTCGAATTGTAGTCTG259560.10631523819798867No Hit
ATTCAGCCCTTTGTCGCTAAGATTCGA258720.10597117593844825No Hit
TCTGATGATTCATGATAACTC257960.10565988151314976No Hit
TGTGCTTGCTAAGTACATG253160.10369381145863311No Hit
ATCTGAAGGTCGCGTGT250390.10255922519800578No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)