FastQCFastQC Report
Thu 22 Jun 2023
DRR395084_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR395084_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24592004
Total Bases561.5 Mbp
Sequences flagged as poor quality0
Sequence length14-135
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGGTGCACCGCCGACCGACCTTG4885381.986572546100757No Hit
CGTTCGAATTGTAGTCTG4610021.8746011915092402No Hit
CTGATGATTCATGATAACTCG3334691.3560057976568318No Hit
CGGGTGCACCGCCGACCGACCTTG2903861.180814707089345No Hit
CTGATGATTCATGATAACTC2522861.025886300278741No Hit
CGTTCGAATTGTAGTCT2521741.0254308676917911No Hit
TTTTACTTACTCCGTGAATCGGA2508191.0199209466621753No Hit
CTGATGATTCATGATAACTCGACGGATCGC2142070.8710432870781902No Hit
CGAGGCAAACTCTGAATACTAGAT1461020.5941036769512562No Hit
CTGATGATTCATGATAACTCGACGGATCGCA1411970.5741581694602848No Hit
TTACGATAGGTGTCAAGTGGA1143420.464956007651918No Hit
TTTTACTTACTCCGTGAATCGG1057920.4301886092731605No Hit
TACTTACTCCGTGAATCG958530.38977303354374865No Hit
ATTTTACTTACTCCGTGAATCGGA910850.37038461769931397No Hit
TTACTTACTCCGTGAATCG878290.3571445417787017No Hit
AGAGAGGGTGAGAGCCCCGTCGTGCCCGG871960.3545705343899586No Hit
GGCTCCCAAAGGCACG827840.336629743554043No Hit
GCGGGTGCACCGCCGACCGACCTT780570.31740804856733107No Hit
GGCTCCCAAAGGCACGT763490.3104627016163465No Hit
TCCACGCTCACCGCACCA748060.30418830445863626No Hit
TTACTTACTCCGTGAATCGG730430.29701930757656025No Hit
TTTTACTTACTCCGTGAATCG723130.29405086303661954No Hit
TATTTTACTTACTCCGTGAATCGGA710780.28902890549302124No Hit
TACCCCGCATCTCCACCA683300.27785454166321705No Hit
ACCCGGGCGAAGCCACCA667830.2715638790559728No Hit
TCGAGGCAAACTCTGAATACTAGAT659400.26813593556669885No Hit
CCTGCGGAAGGATCATTG655510.26655412059952494No Hit
ATAGTTTGTTTGATGGT636280.25873450573609214No Hit
TACTTACTCCGTGAATCGG622550.2531513901835735No Hit
TTTACTTACTCCGTGAATCGGA615640.2503415337765885No Hit
CGAGGGCACGTCTGCCTGGGTGTCACA615230.25017481291886584No Hit
GTTCGAATTGTAGTCTG604830.24594579604004618No Hit
TTTTTACTTACTCCGTGAATCGGA602960.24518538627433537No Hit
TTACTTACTCCGTGAATCGGA568480.23116456877609487No Hit
TACTTACTCCGTGAATCGGA529480.21530575548052125No Hit
TGAGGCAAACTCTGAATACTAGAT514910.20938106548779026No Hit
TACGATAGGTGTCAAGTGGA512940.20857999209824463No Hit
TTACGATAGGTGTCAAGTGGAA508310.20669726631469318No Hit
ACGCATTTATTAGATA506400.20592059109944844No Hit
TTATTTTACTTACTCCGTGAATCGGA495020.20129307070704772No Hit
CTGATGATTCATGATAACT481710.19588074237463526No Hit
TCGCTAAGATTCGA465700.1893704961986831No Hit
TTTACTTACTCCGTGAATCGG464580.1889150636117333No Hit
ACTTACTCCGTGAATCG462120.18791473846539714No Hit
CTGTTTTTGGTCCCAAGGCT455120.18506828479696083No Hit
GCGTTCGAATTGTAGTCTG449300.18270166188977524No Hit
TTGTCGCTAAGATTCGA428440.17421922995783506No Hit
ACGTTATTTTACTTACTCCGTGAATCG425950.17320670572434846No Hit
CTGATGATTCATGATAACTCGA423690.17228770782568187No Hit
ATGGCTCATTAAATCAGTTATAGTTTGTTTGATG409210.16639961509440224No Hit
AGTTTGTTTGATGGT407540.165720532576361No Hit
TACTTACTCCGTGAATC406090.16513091003075633No Hit
AGGCTCCCAAAGGCACGT403530.16408992126058533No Hit
TTTACTTACTCCGTGAATCG403250.1639760631138479No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACCTTG385440.1567338717088693No Hit
TCTGATGATTCATGATAACTCGACGGATCGCA376200.15297655286653336No Hit
ACGTTATTTTACTTACTCCGTGAATCGGA372760.1515777242066161No Hit
AGGCTCCCAAAGGCACG361380.1469502038142154No Hit
ACCCGGGCTCAGACACCA359830.14631991764477592No Hit
TCCCCCGCATCTCCACCA356910.14513253982879964No Hit
CGACCCTCAGTAGGAGCTT345310.14041555946396236No Hit
ATAACTCGACGGATCGC344560.14011058228520132No Hit
CGGGTGCACCGCCGACCGACCTT342160.13913465531316602No Hit
GTGAACCATCGAGT340160.13832138283646994No Hit
GTTCGAATTGTAGTCT338150.13750404399739038No Hit
CGTTCGAATTGTAGTC324420.13192092844487177No Hit
GTCGCTAAGATTCGA323880.13170134487616383No Hit
TCGGGTGCACCGCCGACCGACCTTG323060.13136790316071842No Hit
TTCGAATTGTAGTCTG321430.1307050860922111No Hit
TTTGTCGCTAAGATTCGA317470.12909480658835285No Hit
TAGAATCCTTTGCAGACGACTTAAAT309950.12603690207597557No Hit
TCCCTGTATAGGCCACCA304630.123873597287964No Hit
ATTTTACTTACTCCGTGAATCGG304280.1237312746045422No Hit
ACTTACTCCGTGAATC297320.12090108638563983No Hit
TGTCGCTAAGATTCGA293620.11939653230375208No Hit
ACGTTATTTTACTTACTCCGTGAATC288590.1173511520248614No Hit
GCCCCACGGTGGGCGCCA278830.11338238233858454No Hit
TTGGGCCAACCTATGGTGTACCGGT273720.11130447116062603No Hit
TCGGATAACCGTAGTAATTCTAGAGCTAATACGTGC271700.11048306595916298No Hit
TGATGATTCATGATAACTCG263930.10732350238719869No Hit
TATTTTACTTACTCCGTGAATCGG261170.10620118636935812No Hit
TCTGATGATTCATGATAACTCG257630.10476169408560602No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACCTT251600.10230967756836734No Hit
TAGAATCCTTTGCAGACGACTTAAATA251420.10223648304546469No Hit
GCCTCTCTCACCCCACCA248640.10110603430285713No Hit
ACTTACTCCGTGAATCGG248020.10085391983508135No Hit
TCCCAGTAAAACGCGGCGTGTTCGAATT246040.10004878008315224No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)