FastQCFastQC Report
Thu 22 Jun 2023
DRR395085_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR395085_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16610992
Total Bases377.2 Mbp
Sequences flagged as poor quality0
Sequence length14-135
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGGTGCACCGCCGACCGACCTTG3137501.888809530460312No Hit
CTGATGATTCATGATAACTCG3093291.8621946238972362No Hit
CGTTCGAATTGTAGTCTG3076991.8523818445039284No Hit
TTTTACTTACTCCGTGAATCGGA2964991.7849566118627955No Hit
CTGATGATTCATGATAACTC2453291.476907580233619No Hit
CGGGTGCACCGCCGACCGACCTTG2009011.2094461306103814No Hit
CGTTCGAATTGTAGTCT1644550.9900371994640658No Hit
TACCCCGCATCTCCACCA1172620.7059301455325485No Hit
CGAGGCAAACTCTGAATACTAGAT1121700.675275745121062No Hit
CTGATGATTCATGATAACTCGACGGATCGC1003620.6041902855651247No Hit
ATTTTACTTACTCCGTGAATCGGA1003500.6041180442444376No Hit
AGAGAGGGTGAGAGCCCCGTCGTGCCCGG943030.567714438728283No Hit
TTTTACTTACTCCGTGAATCGG839970.5056711844783262No Hit
TATTTTACTTACTCCGTGAATCGGA820520.49396207041698653No Hit
TACTTACTCCGTGAATCG786460.4734575755620134No Hit
TTTACTTACTCCGTGAATCGGA765760.46099594774351826No Hit
TTTTTACTTACTCCGTGAATCGGA721730.43448940316147283No Hit
GGCTCCCAAAGGCACGT716290.43121446329033214No Hit
CTGATGATTCATGATAACTCGACGGATCGCA708710.4266512198669411No Hit
GGCTCCCAAAGGCACG694570.4181387842459981No Hit
TTACTTACTCCGTGAATCG689810.41527321185874994No Hit
TTACGATAGGTGTCAAGTGGA683340.41137820065171304No Hit
TTACTTACTCCGTGAATCGGA662140.3986155673303557No Hit
TCCCCCGCATCTCCACCA660220.3974597061993649No Hit
TTACTTACTCCGTGAATCGG651460.39218608978921915No Hit
TTATTTTACTTACTCCGTGAATCGGA615280.3704053316021102No Hit
ACGCATTTATTAGATA606190.3649330515600754No Hit
TACTTACTCCGTGAATCGG587780.35385002894468914No Hit
TACTTACTCCGTGAATCGGA566300.340918832541729No Hit
GCGGGTGCACCGCCGACCGACCTT548380.3301307953191477No Hit
TTTTACTTACTCCGTGAATCG519700.3128651196749719No Hit
TCGAGGCAAACTCTGAATACTAGAT506520.30493061461952425No Hit
ACGTTATTTTACTTACTCCGTGAATCGGA493330.2969900894540194No Hit
TCCACGCTCACCGCACCA485980.292565308561945No Hit
AGGCTCCCAAAGGCACGT484350.29158403062261423No Hit
CCTGCGGAAGGATCATTG460400.27716586703551477No Hit
CGAGGGCACGTCTGCCTGGGTGTCACA455360.2741317315666638No Hit
GTTCGAATTGTAGTCTG396260.23855288112835166No Hit
CTGATGATTCATGATAACTCGA395550.2381254533142873No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACCTTG389080.23423044210725044No Hit
TTTACTTACTCCGTGAATCGG369640.22252734815596803No Hit
AGGCTCCCAAAGGCACG349570.2104449872710793No Hit
CTGTTTTTGGTCCCAAGGCT346110.2083620291912729No Hit
ACCCGGGCGAAGCCACCA340020.20469578216641124No Hit
TCCCTGTATAGGCCACCA333450.20074056985880193No Hit
ACTTACTCCGTGAATCG330470.1989465770617432No Hit
TACTTACTCCGTGAATC313300.18861004809345522No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACCTT313060.18846556545208137No Hit
TGAGGCAAACTCTGAATACTAGAT307920.18537122888265795No Hit
CTGATGATTCATGATAACT306500.18451637325452927No Hit
TACGATAGGTGTCAAGTGGA304530.18333041157325222No Hit
TTTACTTACTCCGTGAATCG291280.17535376574740388No Hit
TTACGATAGGTGTCAAGTGGAA289340.17418586439629855No Hit
CGACCCTCAGTAGGAGCTT275760.16601055493856118No Hit
TCGCTAAGATTCGA263410.1585757190178648No Hit
TAGAATCCTTTGCAGACGACTTAAAT258950.15589074993233396No Hit
ACGTTATTTTACTTACTCCGTGAATCG258600.15568004608033043No Hit
TCTGATGATTCATGATAACTCG251920.15165861256209143No Hit
AAGCCATCTCGTACTAAACGA251600.1514659690402596No Hit
TTGTCGCTAAGATTCGA244720.1473241333208757No Hit
CGGGTGCACCGCCGACCGACCTT244090.14694486638726936No Hit
ATTTTACTTACTCCGTGAATCGG243570.14663182066429267No Hit
TGATGATTCATGATAACTCG239220.14401307278939152No Hit
GCGTTCGAATTGTAGTCTG235690.14188797393918437No Hit
ATAGTTTGTTTGATGGT232020.1396785935481758No Hit
ATAACTCGACGGATCGC219160.13193673201456No Hit
CGTTCGAATTGTAGTC212730.12806580124775208No Hit
TAGAATCCTTTGCAGACGACTTAAATA212590.12798151970695065No Hit
TATTTTACTTACTCCGTGAATCGG211270.12718686517939445No Hit
GTTCGAATTGTAGTCT211230.12716278473916548No Hit
ACGTTATTTTACTTACTCCGTGAATCGG207210.1247427004961534No Hit
TTACTTACTCCGTGAATC206550.1243453732323753No Hit
ACTTACTCCGTGAATC202560.12194334931953492No Hit
GTCGCTAAGATTCGA200330.12060086477676951No Hit
TTTTACTTACTCCGTGAATC197600.11895737473114189No Hit
ATCTCTCAGGCGACGCCA197430.11885503286016873No Hit
AGAGGGTGAGAGCCCCGTCGTGCCCGG196680.11840352460587543No Hit
TTCGAATTGTAGTCTG196560.11833128328518851No Hit
GTCGTTGTAGTATAGTGG195000.11739214611625844No Hit
TCTGATGATTCATGATAACTC188920.11373191920145406No Hit
TCCCAGTAAAACGCGGCGTGTTCGAATT186170.11207638893571198No Hit
TCTGATGATTCATGATAACTCGACGGATCGCA185910.11191986607422362No Hit
TTGGGCCAACCTATGGTGTACCGGT184010.11077604516334726No Hit
TGATGATTCATGATAACTC183280.11033657712916844No Hit
ACGTTATTTTACTTACTCCGTGAATC181450.10923489698869279No Hit
TTTGTCGCTAAGATTCGA181370.10918673610823483No Hit
CCTGTGGCTGGATT178850.10766966837380935No Hit
ACTTACTCCGTGAATCGGA177770.10701949648762699No Hit
TGAGGGCACGTCTGCCTGGGTGTCACA177600.10691715461665385No Hit
TGTCGCTAAGATTCGA174630.10512918192965236No Hit
ACTTACTCCGTGAATCGG174500.1050509204989082No Hit
ATGGCTCATTAAATCAGTTATAGTTTGTTTGATG170530.10266093680618231No Hit
TCGGGTGCACCGCCGACCGACCTTG170060.10237799163349184No Hit
CGTTATTTTACTTACTCCGTGAATCGGA169370.101962604039542No Hit
TTATTTTACTTACTCCGTGAATCGG167080.10058399883643313No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)