FastQCFastQC Report
Thu 22 Jun 2023
DRR395086_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR395086_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13180798
Total Bases292.2 Mbp
Sequences flagged as poor quality0
Sequence length14-135
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGGTGCACCGCCGACCGACCTTG4151393.1495741001417366No Hit
CGTTCGAATTGTAGTCTG3643072.7639221843776074No Hit
CTGATGATTCATGATAACTCG2194701.6650736928067633No Hit
CGTTCGAATTGTAGTCT1809481.372815211946955No Hit
CTGATGATTCATGATAACTC1709271.296787948650757No Hit
CGGGTGCACCGCCGACCGACCTTG1309980.9938548485455887No Hit
TTTTACTTACTCCGTGAATCGGA1262840.9580907013368993No Hit
CTGATGATTCATGATAACTCGACGGATCGC1144890.8686044653745548No Hit
AGGCTCCCAAAGGCACGT942960.7154043328787831No Hit
TACTTACTCCGTGAATCGG930370.7058525591546126No Hit
GCGTTCGAATTGTAGTCTG904900.6865289946784708No Hit
TTACGATAGGTGTCAAGTGGA850640.6453630500975738No Hit
TACCCCGCATCTCCACCA848090.643428417611741No Hit
CGAGGCAAACTCTGAATACTAGAT793800.6022397126486575No Hit
TACTTACTCCGTGAATCGGA780010.5917775236370363No Hit
CTGATGATTCATGATAACTCGACGGATCGCA765440.580723564688572No Hit
AGGCTCCCAAAGGCACG708120.5372360611246754No Hit
GGCTCCCAAAGGCACGT702330.5328433073627257No Hit
TACTTACTCCGTGAATCG667410.5063502224979094No Hit
CGAGGGCACGTCTGCCTGGGTGTCACA618700.46939494862147196No Hit
GCGGGTGCACCGCCGACCGACCTT600700.455738719309711No Hit
GGCTCCCAAAGGCACG587740.4459062342052431No Hit
ACGTTATTTTACTTACTCCGTGAATCGGA519050.3937925457927509No Hit
TTACGATAGGTGTCAAGTGGAA515900.3914027056631928No Hit
TCGAGGCAAACTCTGAATACTAGAT488980.3709790560480481No Hit
ATTTTACTTACTCCGTGAATCGGA484770.36778501574790845No Hit
ATTCAGCCCTTTGTCGCTAAGATTCGA480860.36481857926963146No Hit
AGAGAGGGTGAGAGCCCCGTCGTGCCCGG448580.3403284080372069No Hit
TTTTACTTACTCCGTGAATCGG432820.3283716205953539No Hit
TCCCCCGCATCTCCACCA432570.3281819507438017No Hit
ACGCATTTATTAGATA420060.3186908713721278No Hit
TTACTTACTCCGTGAATCGG419260.31808392784716066No Hit
TTACTTACTCCGTGAATCGGA390910.2965753666811372No Hit
CTGTTTTTGGTCCCAAGGCT382650.2903086747858513No Hit
TTGTCGCTAAGATTCGA368110.2792774762195734No Hit
TCGCTAAGATTCGA364800.27676624738502176No Hit
CGACCCTCAGTAGGAGCTT358890.2722824520943269No Hit
TTTACTTACTCCGTGAATCGGA356470.27044644793130124No Hit
ACTTACTCCGTGAATCG353830.26844353429890966No Hit
TATTTTACTTACTCCGTGAATCGGA350900.26622060363871747No Hit
CTGATGATTCATGATAACTCGA343620.2606974175615164No Hit
GCGTTCGAATTGTAGTCT340960.2586793303410006No Hit
GTTCGAATTGTAGTCTG326050.2473674203944253No Hit
TTACTTACTCCGTGAATCG308070.23372636467078853No Hit
CGTTCGAATTGTAGTC305960.23212555112368766No Hit
CTGATGATTCATGATAACT295560.22423528529911468No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACCTTG290000.22001702780059296No Hit
TCTGATGATTCATGATAACTCGACGGATCGCA289550.21967562206779892No Hit
TCAGCCCTTTGTCGCTAAGATTCGA289230.21943284465781204No Hit
TTATTTTACTTACTCCGTGAATCGGA271380.20589041725698246No Hit
CCTGCGGAAGGATCATTG268960.20405441309395683No Hit
TTTGTCGCTAAGATTCGA266390.20210460701999985No Hit
CCGACCCTCAGTAGGAGCTT263410.1998437423894972No Hit
TACGATAGGTGTCAAGTGGA252240.19136929342214334No Hit
TTTTTACTTACTCCGTGAATCGGA248190.18829664182699712No Hit
ACGTTATTTTACTTACTCCGTGAATCGG242410.18391147485910944No Hit
TGAGGCAAACTCTGAATACTAGAT239460.18167337061079306No Hit
ACGTTATTTTACTTACTCCGTGAATCG235650.178782802073137No Hit
AGCCCTTTGTCGCTAAGATTCGA233470.17712888096760152No Hit
TTTTACTTACTCCGTGAATCG226470.17181812512413894No Hit
ATAACTCGACGGATCGC224380.17023248516516223No Hit
TTTCAGCCCTTTGTCGCTAAGATTCGA223830.16981521149174733No Hit
TACTTACTCCGTGAATC217540.16504311802669308No Hit
GTCGCTAAGATTCGA217360.16490655573357546No Hit
TGTCGCTAAGATTCGA210710.159861337682286No Hit
AAGCCATCTCGTACTAAACGA203630.1544898874863267No Hit
TTTACTTACTCCGTGAATCGG200210.1518952039170921No Hit
ACTTACTCCGTGAATCGG198320.15046129983935722No Hit
GCGGGTGCACCGCCGACCGACCT196420.14901980896756023No Hit
TGAGATTCAGCCCTTTGTCGCTAAGATTCGA194190.1473279538917143No Hit
TTCGAATTGTAGTCTG188130.1427303566900881No Hit
ATAACTCGACGGATCGCA186630.1415923375807747No Hit
TCTGATGATTCATGATAACTCG185070.14040879770708875No Hit
TGAGGGCACGTCTGCCTGGGTGTCACA175400.1330723678490483No Hit
GCGCTACACTGATGTATTCAACG171020.12974935204985313No Hit
ACTTACTCCGTGAATC169470.12857339897022926No Hit
GTTCGAATTGTAGTCT166200.12609251731192603No Hit
ATAGTTTGTTTGATGGT166060.12598630219505677No Hit
CGGGTGCACCGCCGACCGACCTT165070.12523520958290993No Hit
CGTTCGAATTGTAGT160820.12201082210652192No Hit
TCGAATTGTAGTCTG159970.12136594461124432No Hit
TCCACGCTCACCGCACCA157080.11917336112730048No Hit
CCTTTGTCGCTAAGATTCGA157060.11915818753917631No Hit
TCGGGTGCACCGCCGACCGACCTTG156980.1190974931866796No Hit
TCGTTCGAATTGTAGTCTG155200.11774704384362768No Hit
ACGTTATTTTACTTACTCCGTGAATC151520.11495510362877878No Hit
TTTACGATAGGTGTCAAGTGGA148380.1125728502932827No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACCTT145620.11047889513214602No Hit
TGATGATTCATGATAACTCG144250.10943950434563977No Hit
TCTGATGATTCATGATAACTCGACGGATCGC142390.10802836065009114No Hit
TAGAATCCTTTGCAGACGACTTAAATA141670.10748211147762071No Hit
GAGATTCAGCCCTTTGTCGCTAAGATTCGA141510.10736072277262729No Hit
TCCCAGTAAAACGCGGCGTGTTCGAATT140920.106913101922964No Hit
CTTTGTCGCTAAGATTCGA139960.10618476969300342No Hit
TCTGATGATTCATGATAACTC137640.10442463347059866No Hit
ATTTTACTTACTCCGTGAATCGG135380.10271001801256646No Hit
TACGATAGGTGTCAAGTGGAA133970.10164028004981185No Hit
ACTTACTCCGTGAATCGGA133750.10147337058044588No Hit
TTTACTTACTCCGTGAATCG132650.10063882323361605No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)