FastQCFastQC Report
Thu 22 Jun 2023
DRR395087_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR395087_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13608090
Total Bases302.3 Mbp
Sequences flagged as poor quality0
Sequence length14-135
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGGTGCACCGCCGACCGACCTTG3499602.5717055075326516No Hit
CGGGTGCACCGCCGACCGACCTTG2343021.7217846148871736No Hit
CGTTCGAATTGTAGTCTG2316171.7020537048182367No Hit
CTGATGATTCATGATAACTCG2066311.518442338344323No Hit
CTGATGATTCATGATAACTC1668651.2262191093680304No Hit
TTTTACTTACTCCGTGAATCGGA1192250.8761332413292386No Hit
GGCTCCCAAAGGCACG1029960.756873301102506No Hit
TTACGATAGGTGTCAAGTGGA985720.7243632280503729No Hit
GGCTCCCAAAGGCACGT984230.7232682911415195No Hit
TACCCCGCATCTCCACCA870980.6400457374987967No Hit
CTGATGATTCATGATAACTCGACGGATCGC861950.6334099789169532No Hit
CGTTCGAATTGTAGTCT776530.5706384951892587No Hit
CGAGGCAAACTCTGAATACTAGAT702100.5159430897355911No Hit
ATTCAGCCCTTTGTCGCTAAGATTCGA681510.5008123843978104No Hit
AGAGAGGGTGAGAGCCCCGTCGTGCCCGG660810.4856008447915909No Hit
GCGGGTGCACCGCCGACCGACCTT656740.48260997685935353No Hit
CGAGGGCACGTCTGCCTGGGTGTCACA648680.4766870295537434No Hit
AGGCTCCCAAAGGCACGT645790.4745632928647592No Hit
TACTTACTCCGTGAATCG640730.4708449165165721No Hit
CTGATGATTCATGATAACTCGACGGATCGCA605180.44472075067110817No Hit
AGGCTCCCAAAGGCACG590910.43423434148363216No Hit
TCCCCCGCATCTCCACCA556160.4086980612268144No Hit
TCGCTAAGATTCGA554330.4073532729427862No Hit
TTACGATAGGTGTCAAGTGGAA531490.3905691393869382No Hit
TACTTACTCCGTGAATCGG530770.39004004235715667No Hit
TTGTCGCTAAGATTCGA508400.3736012915846383No Hit
TTACTTACTCCGTGAATCG467130.3432737437803542No Hit
TCAGCCCTTTGTCGCTAAGATTCGA455290.3345730370683909No Hit
ATTTTACTTACTCCGTGAATCGGA438790.32244789680256375No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACCTTG404210.29703654223333326No Hit
TTTTACTTACTCCGTGAATCGG384490.28254516247320527No Hit
TCGAGGCAAACTCTGAATACTAGAT378210.2779302606023329No Hit
CCTGCGGAAGGATCATTG370170.2720220104364389No Hit
TTACTTACTCCGTGAATCGG367240.26986887946802235No Hit
TTTGTCGCTAAGATTCGA366560.2693691767176731No Hit
TTTCAGCCCTTTGTCGCTAAGATTCGA356520.2619912125801637No Hit
TACTTACTCCGTGAATCGGA344030.25281284882742544No Hit
TTTACTTACTCCGTGAATCGGA343990.2527834545479931No Hit
ACGTTATTTTACTTACTCCGTGAATCGGA333330.24494987907928298No Hit
TATTTTACTTACTCCGTGAATCGGA331110.24331849657078988No Hit
TTACTTACTCCGTGAATCGGA330040.24253219959597563No Hit
TACGATAGGTGTCAAGTGGA326690.24007042869351983No Hit
ACTTACTCCGTGAATCG325570.23924738886941516No Hit
GTCGCTAAGATTCGA322390.23691054365454667No Hit
TGTCGCTAAGATTCGA309490.22743088853762725No Hit
CGGGTGCACCGCCGACCGACCTT306050.22490298050644875No Hit
GCGTTCGAATTGTAGTCTG305500.2244988091642545No Hit
TTTTACTTACTCCGTGAATCG300370.22072899282706096No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACCTT297000.21825252478488896No Hit
TGAGGCAAACTCTGAATACTAGAT286700.2106834978310696No Hit
TGAGGGCACGTCTGCCTGGGTGTCACA286300.21038955503674653No Hit
AGCCCTTTGTCGCTAAGATTCGA282770.20779550987684534No Hit
GCGCTACACTGATGTATTCAACG276590.2032540937045537No Hit
AAGCCATCTCGTACTAAACGA272600.20032201433118094No Hit
ACGTTATTTTACTTACTCCGTGAATCG263330.1935098900727435No Hit
TTATTTTACTTACTCCGTGAATCGGA255160.18750610849869453No Hit
ATAACTCGACGGATCGC249360.18324393798100982No Hit
CTGATGATTCATGATAACTCGA243760.17912873886048666No Hit
CTGTTTTTGGTCCCAAGGCT242160.17795296768319432No Hit
GAGATTCAGCCCTTTGTCGCTAAGATTCGA240050.17640241944314008No Hit
TTTTTACTTACTCCGTGAATCGGA233110.1713025119616346No Hit
GTTCGAATTGTAGTCTG227300.16703299287409182No Hit
CCTTTGTCGCTAAGATTCGA223900.16453447912234562No Hit
TCGGGTGCACCGCCGACCGACCTTG221550.1628075652056975No Hit
AGAGGGTGAGAGCCCCGTCGTGCCCGG213640.15699484644795852No Hit
CTGATGATTCATGATAACT213110.15660537224548046No Hit
ACGTTATTTTACTTACTCCGTGAATC213100.15659802367562237No Hit
ATAACTCGACGGATCGCA210460.15465800123309004No Hit
TGAGATTCAGCCCTTTGTCGCTAAGATTCGA196810.1446272033768148No Hit
CTTTGTCGCTAAGATTCGA196010.14403931778816864No Hit
TAGAATCCTTTGCAGACGACTTAAAT195850.14392174067043942No Hit
TGTGCTTGCTAAGTACATG194140.14266513522470822No Hit
TTTACTTACTCCGTGAATCGG194070.1426136952357017No Hit
TTTACTTACTCCGTGAATCG193510.1422021753236494No Hit
CAGCCCTTTGTCGCTAAGATTCGA190070.13967426729247087No Hit
AGAGCCCCGTCGTGCCCGG188590.13858667895347548No Hit
TCTGATGATTCATGATAACTCGACGGATCGCA186040.13671279363966582No Hit
TACTTACTCCGTGAATC186000.1366833993602335No Hit
CGACCCTCAGTAGGAGCTT185250.13613225662087772No Hit
TCTGATGATTCATGATAACTCG178410.131105834837953No Hit
TTTACGATAGGTGTCAAGTGGA173480.127482989897921No Hit
TTGGGCCAACCTATGGTGTACCGGT171680.12616024732346715No Hit
AGGGTGAGAGCCCCGTCGTGCCCGG170600.12536660177879483No Hit
ATAGTTTGTTTGATGGT169160.12430840771923173No Hit
CCCTTTGTCGCTAAGATTCGA166680.1224859623944286No Hit
TCGCGCTACACTGATGTATTCAACG162800.11963471728949471No Hit
TAGAATCCTTTGCAGACGACTTAAATA162650.11952448874162355No Hit
TACGATAGGTGTCAAGTGGAA162560.11945835161290086No Hit
ACTTACTCCGTGAATC160710.11809886618915659No Hit
TGGCTCCCAAAGGCACG156640.11510799825691923No Hit
AGCCCCGTCGTGCCCGG155460.11424086701366613No Hit
ACGTTATTTTACTTACTCCGTGAATCGG154150.11327820436225804No Hit
TGGCTCCCAAAGGCACGT153640.11290342729949612No Hit
ATCTCTCAGGCGACGCCA151200.1111103762541253No Hit
TGAGAGCCCCGTCGTGCCCGG146920.10796518835486833No Hit
TCCCAGTAAAACGCGGCGTGTTCGAATT145110.10663509721055638No Hit
TTTGCTTGCTAAGTAAATG145040.10658365722154982No Hit
GCGGGTGCACCGCCGACCGACCT140530.10326945221555707No Hit
ACGCATTTATTAGATA138860.1020422410492582No Hit
ATCCGGAGGACCGAG137340.10092525843083049No Hit
ACTTACTCCGTGAATCGG136680.1004402528201974No Hit
TGATGATTCATGATAACTCG136360.10020509858473894No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)