FastQCFastQC Report
Thu 22 Jun 2023
DRR395088_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR395088_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12305213
Total Bases276.5 Mbp
Sequences flagged as poor quality0
Sequence length14-135
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGATTCATGATAACTCG2949752.397154766845564No Hit
GCGGGTGCACCGCCGACCGACCTTG2557492.0783793015204206No Hit
CTGATGATTCATGATAACTC1938721.5755273801436838No Hit
CGTTCGAATTGTAGTCTG1698841.3805856103425436No Hit
CGGGTGCACCGCCGACCGACCTTG1062860.8637477465851261No Hit
TTACGATAGGTGTCAAGTGGA1061680.8627888034120174No Hit
CCTGCGGAAGGATCATTG975030.7923714932850003No Hit
TTTTACTTACTCCGTGAATCGGA903960.7346154836978442No Hit
CGTTCGAATTGTAGTCT871720.7084152058156166No Hit
TACCCCGCATCTCCACCA608010.49410765989991395No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACCTTG581940.4729215170838571No Hit
TACTTACTCCGTGAATCG553570.449866247743944No Hit
GCGGGTGCACCGCCGACCGACCTT543000.44127639237126576No Hit
CGAGGCAAACTCTGAATACTAGAT487310.39601915058276527No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACCTT464920.3778236102048782No Hit
GGCTCCCAAAGGCACG443800.36066015273364227No Hit
TTACTTACTCCGTGAATCG405220.3293075869552197No Hit
AGAGAGGGTGAGAGCCCCGTCGTGCCCGG404090.3283892769674121No Hit
CTGATGATTCATGATAACTCGACGGATCGC399700.3248216832979649No Hit
ATTTTACTTACTCCGTGAATCGGA397260.3228387838552652No Hit
TTACGATAGGTGTCAAGTGGAA371990.3023027720040279No Hit
GTCGCTAAGATTCGA368460.2994340691217616No Hit
TTTTACTTACTCCGTGAATCG366760.2980525408215201No Hit
TTTTACTTACTCCGTGAATCGG358430.2912830521503366No Hit
ACGCATTTATTAGATA354860.28838184271982936No Hit
TACGATAGGTGTCAAGTGGA333580.2710883590556295No Hit
TGAGGCAAACTCTGAATACTAGAT329950.26813838980276083No Hit
TCCACGCTCACCGCACCA326160.26505839435692824No Hit
AAGCCATCTCGTACTAAACGA324990.2641075778208797No Hit
TAGAATCCTTTGCAGACGACTTAAATA323760.2631080014624696No Hit
TCCCCCGCATCTCCACCA321330.26113322865683025No Hit
TACTTACTCCGTGAATCGG313300.25460753909745404No Hit
GGCTCCCAAAGGCACGT312090.25362421601316454No Hit
ACCCGGGCGAAGCCACCA305650.2483906617463672No Hit
TATTTTACTTACTCCGTGAATCGGA300660.24433546985330526No Hit
ACTTACTCCGTGAATCG295070.2397926797366287No Hit
TTACTTACTCCGTGAATCGGA294930.23967890681778525No Hit
TAGAATCCTTTGCAGACGACTTAAAT294660.2394594876171587No Hit
ATGGCTCATTAAATCAGTTATAGTTTGTTTGATG289900.2355912083764824No Hit
TCCCTGTATAGGCCACCA289590.23533928262761483No Hit
CGAGGGCACGTCTGCCTGGGTGTCACA279090.22680631371435828No Hit
TCTGATGATTCATGATAACTCG271840.22091450184568118No Hit
CTGATGATTCATGATAACT270730.22001244513199406No Hit
TACTTACTCCGTGAATCGGA269170.21874468975059594No Hit
ACGTTATTTTACTTACTCCGTGAATCG268800.2184440041793669No Hit
TTTACGATAGGTGTCAAGTGGA258340.20994354181435135No Hit
TTACTTACTCCGTGAATCGG255540.20766808343748297No Hit
ACGTTATTTTACTTACTCCGTGAATCGGA254830.2070910922062056No Hit
CTGATGATTCATGATAACTCGA251590.20445806179868647No Hit
AGGCTCCCAAAGGCACG250170.2033040793361318No Hit
CTGATGATTCATGATAACTCGACGGATCGCA243530.19790799232812956No Hit
GCGTTCGAATTGTAGTCTG240770.19566504049950215No Hit
TTTTTACTTACTCCGTGAATCGGA236460.19216245992653683No Hit
GTTCGAATTGTAGTCTG226550.18410896259983472No Hit
TCGCTAAGATTCGA223610.1817197313041229No Hit
TTATTTTACTTACTCCGTGAATCGGA221240.179793718320845No Hit
TGATGATTCATGATAACTCG217030.17637240411848212No Hit
ATCTCTCAGGCGACGCCA216110.17562475350893966No Hit
TTTACTTACTCCGTGAATCGGA215970.17551098059009623No Hit
TCGAGGCAAACTCTGAATACTAGAT215350.1750071290923611No Hit
AGGCTCCCAAAGGCACGT206950.16818075396175589No Hit
TTGTCGCTAAGATTCGA191270.1554381870512928No Hit
CGTTCGAATTGTAGTC190280.15463364998232862No Hit
CTGTTTTTGGTCCCAAGGCT189290.15382911291336443No Hit
TACTTACTCCGTGAATC182040.1479373010446873No Hit
ACCTGCGGAAGGATCATTG180570.1467426853968314No Hit
TTTACTTACTCCGTGAATCG180440.14663703911504822No Hit
TCTGATGATTCATGATAACTC167480.1361049174849716No Hit
CGACCCTCAGTAGGAGCTT166340.13517848086010376No Hit
ACGTTATTTTACTTACTCCGTGAATC164310.13352877353687417No Hit
ATATGCTTGTCTCAAAGA162970.13243980417080142No Hit
CGGGTGCACCGCCGACCGACCTT158930.12915664279846273No Hit
GTCGTTGTAGTATAGTGG153840.12502018453479838No Hit
GGGGAGGTAGTGACAATAAATAACA152850.1242156474658342No Hit
ACTTACTCCGTGAATC148360.1205667874257845No Hit
TGTCGCTAAGATTCGA147260.11967285734915763No Hit
TGATGATTCATGATAACTC146380.11895771328785612No Hit
TTTACTTACTCCGTGAATCGG146060.1186976609019283No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACCT142650.115926477664385No Hit
GGGAGGTAGTGACAATAAATAACA140690.11433365680057711No Hit
ATCCTGCCGTTCACGCCA140300.1140167179552276No Hit
TTTGTCGCTAAGATTCGA140250.11397608476992638No Hit
ATTTTACTTACTCCGTGAATCG139830.11363476601339612No Hit
TTACGATAGGTGTCAAGTGG138560.11260268310674508No Hit
TTCGAATTGTAGTCTG135550.11015656535161154No Hit
ACGTTATTTTACTTACTCCGTGAATCGG135020.1097258535874186No Hit
AGAATCCTTTGCAGACGACTTAAATA133430.10843371829483976No Hit
TCGGGTGCACCGCCGACCGACCTTG132760.10788923361180339No Hit
TACGATAGGTGTCAAGTGGAA131340.1067352511492487No Hit
TTGGGCCAACCTATGGTGTACCGGT129840.10551625559021205No Hit
TCCCCGGCAACGGCGCCA127460.10358211596987389No Hit
GTCCCGGCAACGGAGCCA126370.10269631253030727No Hit
TGAGGGCACGTCTGCCTGGGTGTCACA124020.10078655282114986No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)