FastQCFastQC Report
Thu 22 Jun 2023
DRR395089_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR395089_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12419288
Total Bases278.4 Mbp
Sequences flagged as poor quality0
Sequence length14-135
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGATTCATGATAACTCG2614202.104951588206989No Hit
CGTTCGAATTGTAGTCTG2471971.99042811471962No Hit
GCGGGTGCACCGCCGACCGACCTTG2413411.9432756531614372No Hit
CTGATGATTCATGATAACTC1887821.5200710378888065No Hit
CGGGTGCACCGCCGACCGACCTTG1376031.1079781707292722No Hit
CGTTCGAATTGTAGTCT1264241.0179649590218054No Hit
TTACGATAGGTGTCAAGTGGA1238020.9968526376069224No Hit
TTTTACTTACTCCGTGAATCGGA892520.7186563352101988No Hit
AGAGAGGGTGAGAGCCCCGTCGTGCCCGG717420.5776659660360561No Hit
GCGGGTGCACCGCCGACCGACCTT666800.536906785638597No Hit
GGCTCCCAAAGGCACGT617120.49690449243145013No Hit
CCTGCGGAAGGATCATTG577250.4648012027742653No Hit
CTGATGATTCATGATAACTCGACGGATCGC549250.4422556268926206No Hit
GGCTCCCAAAGGCACG496350.3996605924590846No Hit
TACTTACTCCGTGAATCG468310.37708280861189464No Hit
CGAGGCAAACTCTGAATACTAGAT457260.3681853581300313No Hit
ATTTTACTTACTCCGTGAATCGGA450510.3627502639442776No Hit
TACGATAGGTGTCAAGTGGA424050.3414446947361234No Hit
AGGCTCCCAAAGGCACGT398670.32100874059768963No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACCTT390270.3142450678331962No Hit
TACCCCGCATCTCCACCA380570.3064346361884836No Hit
GCGTTCGAATTGTAGTCTG370270.29814108506059284No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACCTTG357280.2876815482497869No Hit
CTGATGATTCATGATAACTCGACGGATCGCA354790.2856766023945978No Hit
TTTTACTTACTCCGTGAATCGG345590.2782687703192003No Hit
TTACGATAGGTGTCAAGTGGAA339690.27351809540128225No Hit
CTGATGATTCATGATAACTCGA336550.2709897701059835No Hit
TGAGGCAAACTCTGAATACTAGAT332860.2680185852844382No Hit
TTACTTACTCCGTGAATCG330540.26615052328281624No Hit
CTGTTTTTGGTCCCAAGGCT326450.2628572588058188No Hit
TTTACGATAGGTGTCAAGTGGA321100.25854944341414743No Hit
GTTCGAATTGTAGTCTG318450.2564156656967775No Hit
GTCGCTAAGATTCGA312430.25156836688222384No Hit
CGAGGGCACGTCTGCCTGGGTGTCACA305300.24582729702379075No Hit
ATAGTTTGTTTGATGGT290000.23350775020274914No Hit
TTTTACTTACTCCGTGAATCG285760.23009370585495723No Hit
TACTTACTCCGTGAATCGG275440.2217840507442939No Hit
ACTTACTCCGTGAATCG273670.2203588482689185No Hit
TATTTTACTTACTCCGTGAATCGGA271870.21890948981938418No Hit
CTGATGATTCATGATAACT270990.21820091457738963No Hit
AGGCTCCCAAAGGCACG267930.21573700521318132No Hit
ACGCATTTATTAGATA262660.21149360575260032No Hit
ATGCTTGTCTCAAAGA244920.1972093730333011No Hit
TCGCTAAGATTCGA244070.19652495376546547No Hit
TTACTTACTCCGTGAATCGG241400.1943750720653229No Hit
CGTTCGAATTGTAGTC239950.19320753331430915No Hit
TTGTCGCTAAGATTCGA238270.19185479876141048No Hit
TACTTACTCCGTGAATCGGA236700.19059063611376112No Hit
TCTGATGATTCATGATAACTCG236610.1905181681912844No Hit
TTTTTACTTACTCCGTGAATCGGA235820.18988206087176657No Hit
CGGGTGCACCGCCGACCGACCTT231040.18603320898911435No Hit
AGTTTGTTTGATGGT229600.18487372222948692No Hit
TTACTTACTCCGTGAATCGGA221670.17848849306014966No Hit
TAGAATCCTTTGCAGACGACTTAAAT220500.17754641006795235No Hit
TCCACGCTCACCGCACCA218030.17555756819553583No Hit
ACGTTATTTTACTTACTCCGTGAATCG205930.1658146586181108No Hit
ATGGCTCATTAAATCAGTTATAGTTTGTTTGATG204460.16463101588432447No Hit
TTTACTTACTCCGTGAATCGGA199390.1605486562514695No Hit
TGATGATTCATGATAACTCG195490.15740837961081183No Hit
TCGAGGCAAACTCTGAATACTAGAT194180.1563535687392063No Hit
TTATTTTACTTACTCCGTGAATCGGA194110.15629720479950218No Hit
TCCCCCGCATCTCCACCA188360.15166730975237874No Hit
TACTTACTCCGTGAATC187600.1510553584070198No Hit
ACGTTATTTTACTTACTCCGTGAATCGGA185660.14949327207807725No Hit
TGTCGCTAAGATTCGA184410.14848677315478956No Hit
TCCCTGTATAGGCCACCA177800.14316440684844414No Hit
TAGAATCCTTTGCAGACGACTTAAATA176890.14243167563229067No Hit
AGAGGGTGAGAGCCCCGTCGTGCCCGG176880.14242362364090438No Hit
TTTGTCGCTAAGATTCGA173860.13999192224224127No Hit
ACGTTATTTTACTTACTCCGTGAATC168330.13553917100561644No Hit
TTCGAATTGTAGTCTG162340.1307160281652217No Hit
ACTTACTCCGTGAATC158910.12795419511972023No Hit
CGACCCTCAGTAGGAGCTT158560.12767237542119966No Hit
GTTCGAATTGTAGTCT157610.12690743623950101No Hit
TTACGATAGGTGTCAAGTGG157540.1268510722997969No Hit
TCTGATGATTCATGATAACTC156710.12618275701473386No Hit
TCCCAGTAAAACGCGGCGTGTTCGAATT150870.12148039404513365No Hit
TTGGGCCAACCTATGGTGTACCGGT149590.12044973914768706No Hit
ATATGCTTGTCTCAAAGA148350.11945129221578564No Hit
TTTACTTACTCCGTGAATCG145180.116898810946328No Hit
TCGGGTGCACCGCCGACCGACCTTG144600.1164317954459225No Hit
CCTTTGTCGCTAAGATTCGA141470.11391152214201009No Hit
ATTTTACTTACTCCGTGAATCGG141160.11366191040903471No Hit
ACTTACTCCGTGAATCGGA140370.11302580308951689No Hit
ATAGTTTGTTTGATG140050.11276813936515523No Hit
TTTACTTACTCCGTGAATCGG139500.11232527983890864No Hit
ACCCGGGCGAAGCCACCA139310.1121722920025689No Hit
AGCCCTTTGTCGCTAAGATTCGA139200.1120837200973196No Hit
ATAACTCGACGGATCGC139070.11197904420929766No Hit
AAGCCATCTCGTACTAAACGA135760.10931383506043181No Hit
TGAGGGCACGTCTGCCTGGGTGTCACA133570.10755044894683173No Hit
TGATGATTCATGATAACTC132550.10672914582542897No Hit
AGGGTGAGAGCCCCGTCGTGCCCGG130630.10518316347925903No Hit
ATTCAGCCCTTTGTCGCTAAGATTCGA130460.10504627962569192No Hit
GCGCTACACTGATGTATTCAACG129000.10387068888329186No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)