FastQCFastQC Report
Thu 22 Jun 2023
DRR395090_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR395090_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15883040
Total Bases346.8 Mbp
Sequences flagged as poor quality0
Sequence length14-135
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGGTGCACCGCCGACCGACCTTG3991172.5128501848512625No Hit
CGTTCGAATTGTAGTCTG2265191.4261690457242442No Hit
CTGATGATTCATGATAACTCG2207411.389790619428019No Hit
CGGGTGCACCGCCGACCGACCTTG1883091.1855979711692473No Hit
CTGATGATTCATGATAACTC1222450.7696574459297464No Hit
TTTTACTTACTCCGTGAATCGGA1193550.7514619367576988No Hit
CGTTCGAATTGTAGTCT1030710.6489374830007354No Hit
CGAGGCAAACTCTGAATACTAGAT977920.6157007726480572No Hit
TTACGATAGGTGTCAAGTGGA909170.5724156080951758No Hit
CTGATGATTCATGATAACTCGACGGATCGC791220.49815400578226837No Hit
TACCCCGCATCTCCACCA757060.4766467880204293No Hit
TACTTACTCCGTGAATCG684560.43100061449193605No Hit
TTACTTACTCCGTGAATCGGA674930.4249375434425651No Hit
TTGTCGCTAAGATTCGA591520.3724224078010255No Hit
GGCTCCCAAAGGCACG574690.3618261995184801No Hit
TCGCTAAGATTCGA570170.3589803966998761No Hit
TCGAGGCAAACTCTGAATACTAGAT558100.35138109581037386No Hit
TACTTACTCCGTGAATCGG545390.34337884938903385No Hit
GCGGGTGCACCGCCGACCGACCTT543460.3421637167695857No Hit
ACGCATTTATTAGATA538790.3392234735919572No Hit
ATTCAGCCCTTTGTCGCTAAGATTCGA536080.3375172511055818No Hit
TACTTACTCCGTGAATCGGA526110.33124011524242214No Hit
GGCTCCCAAAGGCACGT525480.33084346573451934No Hit
ACTTACTCCGTGAATCG519160.3268643786076217No Hit
ATTTTACTTACTCCGTGAATCGGA512220.3224949379967563No Hit
TTACGATAGGTGTCAAGTGGAA512160.3224571618531465No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACCTTG499230.31431640290523727No Hit
TTACTTACTCCGTGAATCG486650.3063960047950518No Hit
CTGATGATTCATGATAACTCGACGGATCGCA457250.2878856944262559No Hit
CCTGCGGAAGGATCATTG449480.2829936838287884No Hit
CGAGGGCACGTCTGCCTGGGTGTCACA445270.2803430577521684No Hit
AGAGAGGGTGAGAGCCCCGTCGTGCCCGG437470.275432159082896No Hit
TTACTTACTCCGTGAATCGG428490.26977832958929776No Hit
TTTTACTTACTCCGTGAATCGG426870.2687583737118335No Hit
TGAGGCAAACTCTGAATACTAGAT417530.2628778873565766No Hit
TCCCCCGCATCTCCACCA412700.25983690779598867No Hit
TTTGTCGCTAAGATTCGA409120.2575829312272714No Hit
AGGCTCCCAAAGGCACGT388680.24471385830420372No Hit
AAGCCATCTCGTACTAAACGA380820.23976518349132156No Hit
TTTTACTTACTCCGTGAATCG373020.23485428482204915No Hit
TCAGCCCTTTGTCGCTAAGATTCGA369670.23274511680383606No Hit
AGGCTCCCAAAGGCACG353760.22272814272330738No Hit
TATTTTACTTACTCCGTGAATCGGA330030.2077876779256364No Hit
AGCCCTTTGTCGCTAAGATTCGA324410.2042493124741863No Hit
GTCGCTAAGATTCGA321100.20216532855171304No Hit
GCGTTCGAATTGTAGTCTG315510.1986458511720678No Hit
TTTACTTACTCCGTGAATCGGA311110.1958756006406834No Hit
ACGTTATTTTACTTACTCCGTGAATCG308170.19402456960380382No Hit
TGTCGCTAAGATTCGA306630.19305498191781928No Hit
ACGTTATTTTACTTACTCCGTGAATCGGA305580.19239389940464796No Hit
ATAACTCGACGGATCGC292020.183856490948836No Hit
TCGGGTGCACCGCCGACCGACCTTG277080.1744502311899989No Hit
TACGATAGGTGTCAAGTGGA272380.17149109994056552No Hit
GTTCGAATTGTAGTCTG268600.16911120289314893No Hit
ATCTCTCAGGCGACGCCA264060.166252808026675No Hit
CTGATGATTCATGATAACTCGA258750.1629096193172088No Hit
TTATTTTACTTACTCCGTGAATCGGA255660.16096414792130476No Hit
TTTTTACTTACTCCGTGAATCGGA249430.1570417250098218No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACCTT239860.15101643010406068No Hit
AAAATCCGGAGGACCGAG238730.15030497939940968No Hit
ACTTACTCCGTGAATCGG236040.14861134896090422No Hit
TTTCAGCCCTTTGTCGCTAAGATTCGA232360.14629441215283723No Hit
CTGATGATTCATGATAACT226210.14242235743283402No Hit
TACTTACTCCGTGAATC219100.1379458844150742No Hit
TAGAATCCTTTGCAGACGACTTAAAT214310.13493008895022615No Hit
ATAGTTTGTTTGATGGT209890.1321472463709718No Hit
ACTTACTCCGTGAATC207860.13086915351217399No Hit
TTTACTTACTCCGTGAATCG207760.13080619327282436No Hit
TTTACTTACTCCGTGAATCGG204620.12882924175724547No Hit
GTGAACCATCGAGTCT203630.12820593538768396No Hit
TCTGATGATTCATGATAACTCG203360.12803594274143992No Hit
CCTTTGTCGCTAAGATTCGA202740.12764558925747213No Hit
CGGGTGCACCGCCGACCGACCTT202360.1274063403479435No Hit
ACTTACTCCGTGAATCGGA202090.12723634770169942No Hit
TCCACGCTCACCGCACCA200200.1260463991779911No Hit
TGAGGGCACGTCTGCCTGGGTGTCACA199970.12590159062748693No Hit
GCGCTACACTGATGTATTCAACG198750.12513347570742125No Hit
CGACCCTCAGTAGGAGCTT197460.12432128861981082No Hit
ATCCGGAGGACCGAG197330.12423944030865627No Hit
GAGATTCAGCCCTTTGTCGCTAAGATTCGA195750.12324466852693189No Hit
CGTTCGAATTGTAGTC192380.1211229084608488No Hit
ACGTTATTTTACTTACTCCGTGAATC191590.1206255225699866No Hit
TAGAATCCTTTGCAGACGACTTAAATA190730.12008406451157964No Hit
TGAGATTCAGCCCTTTGTCGCTAAGATTCGA185340.11669050761063374No Hit
GTGAACCATCGAGT182620.11497798910032338No Hit
ACGTTATTTTACTTACTCCGTGAATCGG181780.11444912308978634No Hit
ATCCTGCCGTTCACGCCA179190.1128184528906305No Hit
GTGAACCATCGAGTCTT178940.11266105229225638No Hit
GTGAACCATCGAGTC173820.10943748803755451No Hit
ACCCGGGCGAAGCCACCA171980.10827901963352105No Hit
ATAACTCGACGGATCGCA171220.10780052181446373No Hit
TACGATAGGTGTCAAGTGGAA170980.10764941724002458No Hit
AAAATCCGGAGGACCGA166400.1047658382778108No Hit
GAGGCAAACTCTGAATACTAGAT166140.1046021416555017No Hit
TCTGATGATTCATG164880.10380884263969617No Hit
CTTTGTCGCTAAGATTCGA162400.10224742870382494No Hit
TTTACGATAGGTGTCAAGTGGA161800.10186966726772707No Hit
AAATCCGGAGGACCGAG161610.10175004281296277No Hit
TGCCACGATCCACT158960.10008159647019715No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)