FastQCFastQC Report
Thu 22 Jun 2023
DRR395091_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR395091_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10743807
Total Bases239 Mbp
Sequences flagged as poor quality0
Sequence length14-135
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGGTGCACCGCCGACCGACCTTG2498272.325311688864106No Hit
CTGATGATTCATGATAACTCG1629041.5162595530615917No Hit
CGTTCGAATTGTAGTCTG1396391.2997161992950916No Hit
CGGGTGCACCGCCGACCGACCTTG1190651.1082198330628983No Hit
CGAGGCAAACTCTGAATACTAGAT957440.8911552487865799No Hit
TTACGATAGGTGTCAAGTGGA955070.8889493268075274No Hit
CTGATGATTCATGATAACTCGACGGATCGC854810.7956304501746914No Hit
CGTTCGAATTGTAGTCT835080.7772663823912697No Hit
GGCTCCCAAAGGCACGT767450.7143184906430281No Hit
CTGATGATTCATGATAACTC741280.6899602719966954No Hit
AGGCTCCCAAAGGCACGT738590.6874565040120322No Hit
TTTTACTTACTCCGTGAATCGGA636160.5921178591536501No Hit
CTGATGATTCATGATAACTCGACGGATCGCA575000.5351920413313456No Hit
TCGAGGCAAACTCTGAATACTAGAT545890.5080973625084665No Hit
TCGCTAAGATTCGA534610.49759829081069684No Hit
ATTCAGCCCTTTGTCGCTAAGATTCGA501550.46682707535606327No Hit
AGAGAGGGTGAGAGCCCCGTCGTGCCCGG486160.4525025440237339No Hit
GGCTCCCAAAGGCACG484670.45111569856011No Hit
GCGGGTGCACCGCCGACCGACCTT466320.4340360916758836No Hit
CGAGGGCACGTCTGCCTGGGTGTCACA455260.42374178910697113No Hit
TTGTCGCTAAGATTCGA429320.3995976472771709No Hit
AGGCTCCCAAAGGCACG371520.34579921251377654No Hit
TTACGATAGGTGTCAAGTGGAA360160.3352256793146042No Hit
GCGTTCGAATTGTAGTCTG341970.31829499543318307No Hit
ATTTTACTTACTCCGTGAATCGGA336140.31286861351846695No Hit
ATAACTCGACGGATCGC325840.3032816952128794No Hit
TCAGCCCTTTGTCGCTAAGATTCGA324720.30223923419324267No Hit
ATAGTTTGTTTGATGGT323860.3014387730531645No Hit
TACTTACTCCGTGAATCGGA311180.2896366250808489No Hit
TACTTACTCCGTGAATCG309630.2881939334911731No Hit
GTCGCTAAGATTCGA305870.28469424292524986No Hit
TTTGTCGCTAAGATTCGA303080.2820973980638334No Hit
TGAGGCAAACTCTGAATACTAGAT296880.2763266317051302No Hit
TGTCGCTAAGATTCGA282180.2626443308223984No Hit
TACTTACTCCGTGAATCGG276930.2577577947928514No Hit
TACCCCGCATCTCCACCA270490.25176364392994033No Hit
TACGATAGGTGTCAAGTGGA265000.24665372339618533No Hit
CTGATGATTCATGATAACTCGA260810.24275380226022303No Hit
AGCCCTTTGTCGCTAAGATTCGA257930.24007318820972864No Hit
ATAACTCGACGGATCGCA257110.23930995782035178No Hit
CTGTTTTTGGTCCCAAGGCT247420.23029080846295918No Hit
TTTTACTTACTCCGTGAATCGG247100.22999296245734868No Hit
TTTCAGCCCTTTGTCGCTAAGATTCGA233900.21770681472591605No Hit
ACTTACTCCGTGAATCG232090.21602212325668174No Hit
CGACCCTCAGTAGGAGCTT225730.2101024338951733No Hit
TATTTTACTTACTCCGTGAATCGGA222450.20704951233766578No Hit
GCGCTACACTGATGTATTCAACG212730.1980024399172472No Hit
ACGTTATTTTACTTACTCCGTGAATCGGA202750.18871336761727012No Hit
CCTTTGTCGCTAAGATTCGA200250.18638644569843818No Hit
CCTGCGGAAGGATCATTG194270.1808204484685922No Hit
TTACTTACTCCGTGAATCGGA192090.17879137255537073No Hit
TCTGATGATTCATGATAACTCGACGGATCGCA185280.17245283724847255No Hit
TTTACGATAGGTGTCAAGTGGA182580.16993976157613405No Hit
TCCCCCGCATCTCCACCA176810.16456922578746994No Hit
CTTTGTCGCTAAGATTCGA168130.15649015288528545No Hit
AGAGGGTGAGAGCCCCGTCGTGCCCGG167830.1562109222550256No Hit
TTATTTTACTTACTCCGTGAATCGGA166710.1551684612353889No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACCTTG165370.15392123108689498No Hit
ACGTTATTTTACTTACTCCGTGAATCG164860.15344653901545327No Hit
TGTGCTTGCTAAGTACATG161070.14991892538650406No Hit
ACGCATTTATTAGATA160400.1492953103122571No Hit
TTACTTACTCCGTGAATCGG159140.14812254166516578No Hit
TTACTTACTCCGTGAATCG158190.14723831133600965No Hit
CCCTTTGTCGCTAAGATTCGA153770.1431243133835148No Hit
TTTTACTTACTCCGTGAATCG151930.1414116988512545No Hit
CGGGTGCACCGCCGACCGACCTT150990.1405367762097737No Hit
AGTTTGTTTGATGGT150200.1398014688834228No Hit
CAGCCCTTTGTCGCTAAGATTCGA149460.13911269999544854No Hit
GTTCGAATTGTAGTCTG148010.137763085282526No Hit
TTTTTACTTACTCCGTGAATCGGA144880.13484977904014842No Hit
TTTGCTTGCTAAGTAAATG143180.1332674721353427No Hit
TGAGGGCACGTCTGCCTGGGTGTCACA142520.13265316474877106No Hit
CGTTCGAATTGTAGTC142220.13237393411851126No Hit
TCTGATGATTCATGATAACTCG141060.13129424234817322No Hit
TTTACTTACTCCGTGAATCGGA140820.13107085784396535No Hit
AGGGTGAGAGCCCCGTCGTGCCCGG138660.1290603973060946No Hit
CTGATGATTCATGATAACT138370.12879047436351007No Hit
TCCCAGTAAAACGCGGCGTGTTCGAATT132830.1236340153913785No Hit
ACGTTATTTTACTTACTCCGTGAATC132520.12334547707344333No Hit
TCCACGCTCACCGCACCA129480.12051594002014371No Hit
ATCCGGAGGACCGAG125970.11724894164610365No Hit
TACTTACTCCGTGAATC125400.11671840344860997No Hit
TCGGGTGCACCGCCGACCGACCTTG124450.11583417311945383No Hit
TCGCGCTACACTGATGTATTCAACG123430.11488478897657042No Hit
TTGGGCCAACCTATGGTGTACCGGT121070.11268817468519307No Hit
TAGAATCCTTTGCAGACGACTTAAAT120160.11184117510673823No Hit
GCGGGTGCACCGCCGACCGACCT119490.11121756003249128No Hit
TAGTTTGTTTGATGGT117860.10970040694141285No Hit
CTTACTCCGTGAATCGGA117450.10931879174672442No Hit
CCGACCCTCAGTAGGAGCTT115880.10785748478169795No Hit
TTTTGTTTGATGGT115610.10760617721446411No Hit
ACTTACTCCGTGAATC114970.10701048520324313No Hit
GAGGCAAACTCTGAATACTAGAT114780.10683363913741191No Hit
AGAGCCCCGTCGTGCCCGG112970.10514894766817759No Hit
AAATCCGGAGGACCGAG111040.10335256394683934No Hit
AAAATCCGGAGGACCGAG108110.1006254114579683No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACCTT107820.10035548851538378No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)