FastQCFastQC Report
Thu 22 Jun 2023
DRR428600_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR428600_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15783800
Total Bases789.1 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG243675815.438348179779268TruSeq Adapter, Index 12 (100% over 49bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAG10309006.531380275979169No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA7675314.862777024544153No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT5168003.274243211393961Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT5010203.174267286711692Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAGC4225892.6773590643571255No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACAC2285521.4480163205311776No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA2023351.281915635018183No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA1517600.9614921628505178No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG1500270.9505125508432697Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACAC1054340.6679886972718864No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCG1029600.652314398307125TruSeq Adapter, Index 12 (100% over 50bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGA978400.6198760754697855No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGGAGATCGGAAGAGCACACGTC951620.6029093120794738No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC942310.5970108592354185Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACG936230.5931588083984844No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGGGAGATCGGAAGAGCACACGT902360.5717000975683929No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC882250.5589591860008363No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGAGATCGGAAGAG845380.5355997921919944No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC820320.5197227537094996No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCAC790240.5006652390425627No Hit
CACATATACTAAAATTGGAACGATACAGAGAAAGATCGGAAGAGCACACG784960.497320036999962No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT632750.40088571826809766Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAG624360.39557014153752584No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG526680.333683903749414No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC492910.312288549018614No Hit
AAGATTAGCATGGCCCCTGCGCAAGGATGACAAGATCGGAAGAGCACACG473540.3000164725858158No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAGC468190.29662692127371104No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC459960.2914127143020058TruSeq Adapter, Index 12 (100% over 50bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC449990.2850961112026255No Hit
ACATATACTAAAATTGGAACGATACAGAGAAAGATCGGAAGAGCACACGT439090.27819029637983245No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC435490.275909476805332TruSeq Adapter, Index 12 (100% over 50bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG425470.2695611956563059No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACAGATCGGAAGAGCAC411450.26067867053561244No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACAC408700.2589363778050913No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACA404170.25606634650717824No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACG353860.22419189295353464No Hit
AGATTAGCATGGCCCCTGCGCAAGGATGACAAGATCGGAAGAGCACACGT337440.21378882144984096No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACG306520.19419911554885388No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGT305620.19362891065522878TruSeq Adapter, Index 12 (100% over 50bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT301200.19082857106653658No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG297240.1883196695345861Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAGCACACGTCTGAA290790.1842332011302728Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAG280410.17765683802379656No Hit
GGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCACACGTCTGAACT279360.17699159898123393Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACACGTCTGA278910.17670649653442136Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGC278310.1763263599386713No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACAC256870.16274281225053536No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA249040.1577820296759969No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACAC244950.15519076521496725No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA227880.14437587906587768No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC225000.14255122340627732No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAGCA198640.1258505556329908No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGT198160.12554644635639073No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT190080.12042727353362309No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCAGATCGGAAGAGC188630.11950861009389374No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG184260.11673994855484737No Hit
GGGCTTCTGTCGTGAGTGGCACACGTAGGGAGATCGGAAGAGCACACGTC180000.11404097872502186No Hit
AATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC179830.11393327335622601TruSeq Adapter, Index 12 (98% over 50bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCACAC177520.11246974746258823No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC167390.10605177460434118No Hit
CCCCACAACCGCGCTTGACTAGCTTGCTGTTTAGATCGGAAGAGCACACG162520.1029663325688364No Hit
CCGTCTCCGCCCCCCGGCCCCGCGAGATCGGAAGAGCACACGTCTGAACT161410.10226307986669876Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)