FastQCFastQC Report
Thu 22 Jun 2023
DRR428601_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR428601_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15396377
Total Bases769.8 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG237487315.424882100509748TruSeq Adapter, Index 12 (100% over 49bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAG9981436.482973234547322No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA7433904.8283437070942075No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT4993033.2429902177635688Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT4840643.1440123868102217Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAGC4072542.6451287858175987No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACAC2216321.4395074893268722No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA1947581.2649599318073337No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA1460070.9483205042329114No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG1452940.9436895446246867Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACAC1017560.6609087319698653No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCG1002110.6508739036462928TruSeq Adapter, Index 12 (100% over 50bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGA948780.6162358845850553No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGGAGATCGGAAGAGCACACGTC921590.5985758857424704No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACG910240.59120402156949No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC904140.5872420505161702Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGGCTTCTGTCGTGAGTGGCACACGTAGGGAGATCGGAAGAGCACACGT874050.5676984916646299No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC854860.5552345204329564No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGAGATCGGAAGAG818990.5319368316325328No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC791450.5140495065819706No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCAC763330.4957854695296172No Hit
CACATATACTAAAATTGGAACGATACAGAGAAAGATCGGAAGAGCACACG758430.4926029026179341No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT610140.39628803581517913Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAG603860.3922091541406137No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG508970.33057777164069185No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC475460.308812910985487No Hit
AAGATTAGCATGGCCCCTGCGCAAGGATGACAAGATCGGAAGAGCACACG462770.30057071218767895No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAGC451260.2930949274624803No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC446970.2903085576561291TruSeq Adapter, Index 12 (100% over 50bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC432190.28070889664497045No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC424100.2754544137234363TruSeq Adapter, Index 12 (100% over 50bp)
ACATATACTAAAATTGGAACGATACAGAGAAAGATCGGAAGAGCACACGT423000.27473995992693606No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG408670.26543257546889115No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACAC395640.25696954549761936No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACAGATCGGAAGAGCAC393200.2553847570762914No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACA386380.2509551435379895No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACG340550.22118840036198126No Hit
AGATTAGCATGGCCCCTGCGCAAGGATGACAAGATCGGAAGAGCACACGT330050.21436861412266017No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACG296070.1922984868453143No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGT295470.191908784774496TruSeq Adapter, Index 12 (100% over 50bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT291020.18901849441592655No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG290870.18892106889822197Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAGCACACGTCTGAA280960.1824844896952056Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAG273550.17767166912059895No Hit
GGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCACACGTCTGAACT269590.17509963545319784Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGC267340.17363825268762906No Hit
ATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACACGTCTGA267020.17343041158319258Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACAC251200.16315526698261545No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA238710.15504296887508015No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACAC236940.15389334776616603No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC221330.1437545988903753No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA220990.14353376771691156No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAGCA193780.12586077880529944No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGT192910.12529571080261284No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT185230.12030752429613797No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCAGATCGGAAGAGC177310.11516345696133577No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG176180.11442951806129455No Hit
GGGCTTCTGTCGTGAGTGGCACACGTAGGGAGATCGGAAGAGCACACGTC172650.11213677087797992No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCACAC171810.11159118797883424No Hit
AATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC171490.1113833468743978TruSeq Adapter, Index 12 (98% over 50bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC162450.10551183567406801No Hit
CCCCACAACCGCGCTTGACTAGCTTGCTGTTTAGATCGGAAGAGCACACG158500.10294629704118052No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)