FastQCFastQC Report
Thu 22 Jun 2023
DRR428602_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR428602_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16732926
Total Bases836.6 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG523876331.308110727316908TruSeq Adapter, Index 2 (100% over 49bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG5928173.5428173171864863Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT4502712.6909280540653797Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAG3273761.956477904701186No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT2774061.6578451371864071Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCG2546781.5220171295803255TruSeq Adapter, Index 2 (100% over 50bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA2064601.2338547364639036No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACAC1955331.168552350019357No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC1313860.7851944125014357Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACATATACTAAAATTGGAACGATACAGAGAAAGATCGGAAGAGCACACGT1266310.7567773860949364No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG1211060.7237586540453236No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAGC1163840.6955388435949577No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC1067510.6379697131272798TruSeq Adapter, Index 2 (100% over 50bp)
CCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACG991410.5924905183946908No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC942930.5635177015663608TruSeq Adapter, Index 2 (100% over 50bp)
CACATATACTAAAATTGGAACGATACAGAGAAAGATCGGAAGAGCACACG908780.5431088382270979No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGGAGATCGGAAGAGCACACGTC854000.5103709895089478No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT790250.4722724525286253No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACAC618040.36935560463244743No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACAC608920.3639052727538507No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGT585760.3500642983779406TruSeq Adapter, Index 2 (100% over 50bp)
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG584220.3491439572493179Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTAAAATTGGAACGATACAGAGAAAGATCGGAAGAGCACACGTCTGAACT482820.288544872546499Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT460490.27519992618147Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG459740.27475170809934857Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCAC456260.2726719761983051No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG445170.2660443248240027No Hit
GCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT436750.26101232982205264Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC420800.2514802252756033TruSeq Adapter, Index 2 (98% over 50bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGAGATCGGAAGAG419480.25069136145106957No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGGGAGATCGGAAGAGCACACGT418030.24982480649230146No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACG389180.23258335093336335No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC370920.22167073469398No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT367030.21934597690804347No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA351590.21011866065743673No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACA351390.2099991358355377No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACAC326920.19537527387618875No Hit
ACTAAAATTGGAACGATACAGAGAAAGATCGGAAGAGCACACGTCTGAAC303640.18146258460713924Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTCT302000.18048248106756704Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC287960.17209183857025365No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA275150.16443627372761943No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACAGATCGGAAGAGCAC271430.16221311204029706No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACG265150.1584600326326669No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACAAGATCGGAAGAGCACACG264580.1581193868902546No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACG253320.151390139417338No Hit
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTAT250040.14942993233819357TruSeq Adapter, Index 2 (100% over 48bp)
TAAAATTGGAACGATACAGAGAAAGATCGGAAGAGCACACGTCTGAACTC237170.14173851004898963Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCACACGTCTGAACT230730.1378898107838402Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC230640.1378360246139856No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT220820.13196735585874222No Hit
AGATAGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG209820.12539349065429442TruSeq Adapter, Index 2 (97% over 49bp)
GGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAGCACACGTCTGAA206100.12317032896697207Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG187120.11182742336875212No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAGC180010.10757831595024085No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT178700.10679542836680209No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC178360.1065922361695737No Hit
AGATCGGAAGAGCACACGTCTGAACTCAAGTCACCGATGTATCTCGTATG178010.10638306773125036TruSeq Adapter, Index 2 (97% over 49bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCACAC176720.10561213263000147No Hit
GGGCTTCTGTCGTGAGTGGCACACGTAGGAGATCGGAAGAGCACACGTCT175900.10512208086021536Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAGATCGGAAGAGCACACGT168830.10089687840608391No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)