FastQCFastQC Report
Thu 22 Jun 2023
DRR428603_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR428603_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16345221
Total Bases817.2 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG510556531.23582727942314TruSeq Adapter, Index 2 (100% over 49bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG5731803.506713063102665Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT4354542.664105918176328Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAG3169381.9390254802917624No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT2687321.6441013553747608Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCG2483461.5193798847993552TruSeq Adapter, Index 2 (100% over 50bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA2014081.2322133790665786No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACAC1883881.152557068515623No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC1265330.7741284134365635Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACATATACTAAAATTGGAACGATACAGAGAAAGATCGGAAGAGCACACGT1234290.7551381532253373No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG1165960.7133338851765907No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAGC1120840.6857294863128495No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC1034480.6328944711117702TruSeq Adapter, Index 2 (100% over 50bp)
CCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACG957960.5860795641735281No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC919460.5625252787955574TruSeq Adapter, Index 2 (100% over 50bp)
CACATATACTAAAATTGGAACGATACAGAGAAAGATCGGAAGAGCACACG875820.5358263433697226No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGGAGATCGGAAGAGCACACGTC826990.5059521679150132No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT760170.4650717172927793No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACAC594630.36379440816370734No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACAC583220.3568137744971451No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGT565610.34603998318529927TruSeq Adapter, Index 2 (100% over 50bp)
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG565420.3459237412574599Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTAAAATTGGAACGATACAGAGAAAGATCGGAAGAGCACACGTCTGAACT465110.28455412135449254Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG444000.27163903137192213Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCAC443870.2715594974212952No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT440580.26954667667081406Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG432090.26435249789525633No Hit
GCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT415470.25418438820741546Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC405330.2479807400585162TruSeq Adapter, Index 2 (98% over 50bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGAGATCGGAAGAG405030.24779720017245407No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGGGAGATCGGAAGAGCACACGT404010.2471731645598429No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACG376070.23007948317125843No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC359530.21996031745303413No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT355750.21764771488865156No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA340560.20835447865770676No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACA338060.20682497960718915No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACAC317030.19395883359423527No Hit
ACTAAAATTGGAACGATACAGAGAAAGATCGGAAGAGCACACGTCTGAAC296260.18125175548253525Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTCT293350.17947141858773277Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC280780.17178109736173036No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA265660.16253068710420007No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACAGATCGGAAGAGCAC261570.1600284266575533No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACAAGATCGGAAGAGCACACG256510.1569327205793057No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACG254770.15586818924014548No Hit
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTAT243770.14913839341786814TruSeq Adapter, Index 2 (100% over 48bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACG242920.14861836374069215No Hit
TAAAATTGGAACGATACAGAGAAAGATCGGAAGAGCACACGTCTGAACTC228800.13997975310336888Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC224430.13730618876306414No Hit
GGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCACACGTCTGAACT222150.1359112856289921Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGATAGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG217230.1329012314975735TruSeq Adapter, Index 2 (97% over 49bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT212570.13005024526740874No Hit
GGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAGCACACGTCTGAA205690.12584106388038435Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAAGAGCACACGTCTGAACTCAAGTCACCGATGTATCTCGTATG186200.11391708928254932TruSeq Adapter, Index 2 (97% over 49bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG180880.1106623153030479No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT173030.10585968828442271No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCACAC172430.1054926085122985No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAGC172340.10543754654647987No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC172180.10533965860724673No Hit
GGGCTTCTGTCGTGAGTGGCACACGTAGGAGATCGGAAGAGCACACGTCT168910.10333907384916974Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)