FastQCFastQC Report
Thu 22 Jun 2023
DRR428604_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR428604_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10259058
Total Bases512.9 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG112773410.992568713423786TruSeq Adapter, Index 10 (100% over 49bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG4145744.041053281890013Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT3857833.7604134804579523Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA2647822.5809582127325923No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT1628651.5875239227617195Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA1521851.4834207975040203Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA945440.9215660931052343Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG900260.8775269620271179Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT819860.7991571935746927No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAAGATCGGAAGAGCACA791280.771298885336256No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG644560.6282838053942185No Hit
GGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA545340.5315692727343972Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACAGATCGGAAGAGCAC501800.48912872897297194No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAGATCGGAAGAGCACAC492610.4801707915093179No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT487680.47536528207560574No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG472600.4606660767489569Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGCATTTATCAGATCAAAACCAACCCGGAGATCGGAAGAGCACACGTCT464630.4528973322891829Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACG423130.4124452751899833No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAG404750.39452940026267513No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAGC389400.3795670128777905No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCAC342370.33372459732657717No Hit
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA308900.30109976958898177Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC297980.29045551745589115No Hit
ATGTGTGACTGAAAGGTATTTTCTGAGCAGATCGGAAGAGCACACGTCTG246770.24053865374384278Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGAGATCGGAAGAGCACACGT208770.20349821591806966No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG195700.1907582548027314No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACAC195070.19014416333351464No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGAGATCGGAAGAGCACACG190280.18547511867073957No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC185350.1806696092370274No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT178130.17363192605013053No Hit
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA173840.1694502555692735Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGCATTTATCAGATCAAAACCAACCCGGTAGATCGGAAGAGCACACGTC160900.15683701174123393No Hit
TGCGTGCATTTATCAGATCAAAACCAAAGATCGGAAGAGCACACGTCTGA151960.1481227613685389Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAGCACA148400.14465265719328227No Hit
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCAGATCGGAAGAGCACACGTC147710.14398008082223535No Hit
TGCGTGCATTTATCAGATCAAAACCAACCAGATCGGAAGAGCACACGTCT144220.14057820903244722Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGCGTGCATTTATCAGATCAAAACCAAAGATCGGAAGAGCACACGTCTG141970.13838502521381593Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCT141430.1378586610973444Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAAC139670.1361431039769928Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGAA136530.13308239411454736Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA130540.1272436514151689Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGCGTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCTG123190.12007925094097334Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC122330.11924096734807425TruSeq Adapter, Index 10 (100% over 50bp)
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA121140.11808101679510925Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCCCGCCACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCT117510.11454268023438409Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCG116050.11311954762318335TruSeq Adapter, Index 10 (100% over 50bp)
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGC115150.11224227409573082No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC113440.11057545439357103TruSeq Adapter, Index 10 (100% over 50bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT111950.1091230793314552No Hit
GCTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTCT111840.10901585701143322Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT107750.10502913620334343Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTGA105770.10309913444294788Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGCGTGCATTTATCAGATCAAAACCAACCAGATCGGAAGAGCACACGTC105660.10299191212292591No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGGAGATCGGAAGAGCACACGTC103640.10102292042797692No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)