FastQCFastQC Report
Thu 22 Jun 2023
DRR428606_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR428606_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10723366
Total Bases536.1 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG129901512.113873572906119TruSeq Adapter, Index 1 (100% over 49bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG6755416.299710370792157Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT5909945.511273232677127Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT2964782.764784863260286Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA1978621.8451482491598252No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG1501131.3998682876253594Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA924780.862397124186566Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA811730.756973136979564Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG759990.7087233616758022Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA664350.6195349482615813Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGCATTTATCAGATCAAAACCAACCCGGAGATCGGAAGAGCACACGTCT645630.6020777431265518Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAAGATCGGAAGAGCACA640410.5972098686177455No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT607500.5665198781800416No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC535890.4997404732804979No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACG440510.410794521048708No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACAGATCGGAAGAGCAC413230.38535474775364376No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT389870.363570543055231No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAGC337270.3145187807634282No Hit
ATGTGTGACTGAAAGGTATTTTCTGAGCAGATCGGAAGAGCACACGTCTG324030.302171911319636Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAG313080.291960565367255No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGAGATCGGAAGAGCACACG291770.2720880738380095No Hit
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA289760.27021366238921624Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGCGTGCATTTATCAGATCAAAACCAACCAGATCGGAAGAGCACACGTCT252670.2356256421724298Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA243800.22735398567949655Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGCATTTATCAGATCAAAACCAACCCGGTAGATCGGAAGAGCACACGTC242920.22653334783126863No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCT227610.21225611435812228Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCAC211000.19676657497282105No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT202070.18843896589932674No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCAGATCGGAAGAGCACACGTC198370.18498855676473228No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC196850.18357109139052047No Hit
ACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGAA191340.17843277940900273Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGCGTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCT182230.16993731259382547Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC173660.16194541900369716TruSeq Adapter, Index 1 (100% over 50bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACAC167010.15574400799152058No Hit
GGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTGA152110.1418491171522076Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC148620.1385945420495766TruSeq Adapter, Index 1 (100% over 50bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCG145650.1358248893118075TruSeq Adapter, Index 1 (100% over 50bp)
AGGGCTTCTGTCGTGAGTGGCACACGTAGGAGATCGGAAGAGCACACGTC134090.1250446921237231No Hit
ATGCGTGCATTTATCAGATCAAAACCAAAGATCGGAAGAGCACACGTCTG133310.12431730857643018Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTG123160.1148519970315291Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT117910.1099561462324423No Hit
CCCCGCCACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCT115510.10771804301000264Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATGGATTTTTGGAGCAGGGAGATGGAAGATCGGAAGAGCACACGTCTG115010.1072517715053277Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT113470.10581565527092891Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTGATGATGACCCCAGGTAACTCTGAGTAGATCGGAAGAGCACACGTCT113190.10555454322831097Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA108890.10154460828810655Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTCT108780.10144202855707805Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGT108550.1012275436649276No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)