FastQCFastQC Report
Thu 22 Jun 2023
DRR428607_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR428607_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10906452
Total Bases545.3 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG131511712.05815603461144TruSeq Adapter, Index 1 (100% over 49bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG6896916.32369720235325Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT6033725.532248250851881Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT3042112.7892755590910774Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA2017901.8501892274407845No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG1546471.4179404998069034Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA949330.8704297236168095Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA833300.764043155372618Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG780070.7152371825411233Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA679400.6229340210730309Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGCATTTATCAGATCAAAACCAACCCGGAGATCGGAAGAGCACACGTCT662230.6071910461807378Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAAGATCGGAAGAGCACA652570.5983339036379567No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT622920.571148160740083No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC553090.5071218394396272No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACG449340.41199466150861896No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACAGATCGGAAGAGCAC422340.3872386730350072No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT398470.3653525454474104No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAGC348130.31919638027105424No Hit
ATGTGTGACTGAAAGGTATTTTCTGAGCAGATCGGAAGAGCACACGTCTG334570.3067633727265292Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAG317170.29080951348797945No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGAGATCGGAAGAGCACACG299630.2747272898647516No Hit
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA295960.2713623092092644Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGCGTGCATTTATCAGATCAAAACCAACCAGATCGGAAGAGCACACGTCT262600.24077491011742408Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA245130.2247568686865353Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGCATTTATCAGATCAAAACCAACCCGGTAGATCGGAAGAGCACACGTC242750.2225746741470095No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCT229710.210618448602717Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCAC215900.19795621894269558No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT206860.18966754724634555No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCAGATCGGAAGAGCACACGTC202570.18573409574442723No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC200150.18351522566642203No Hit
ACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGAA196010.17971930743380157Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGCGTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCT179470.16455397227255938Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC175010.16046464973210353TruSeq Adapter, Index 1 (100% over 50bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACAC172570.1582274418848586No Hit
GGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTGA154100.14129251199198417Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC152160.1395137483757321TruSeq Adapter, Index 1 (100% over 50bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCG147030.13481011056574585TruSeq Adapter, Index 1 (100% over 50bp)
ATGCGTGCATTTATCAGATCAAAACCAAAGATCGGAAGAGCACACGTCTG137210.12580626586904706Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGCTTCTGTCGTGAGTGGCACACGTAGGAGATCGGAAGAGCACACGTC137020.1256320570612698No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTG124250.1139233914017134Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT119670.10972404224581925No Hit
CCCCGCCACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCT119370.10944897570722358Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATGGATTTTTGGAGCAGGGAGATGGAAGATCGGAAGAGCACACGTCTG116680.10698254574448227Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTGATGATGACCCCAGGTAACTCTGAGTAGATCGGAAGAGCACACGTCT115020.10546051089758612Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT113680.10423188035852539Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTCT111790.10249896116537258Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGT109610.10050014431824393No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGGGAGATCGGAAGAGCACACGT109180.10010588227959011No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)