FastQCFastQC Report
Mon 26 Jun 2023
ERR10323163_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR10323163_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18171762
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG800150.44032603992942454No Hit
CCTTGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG573250.31546197886589095No Hit
ACGGACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC417210.22959248530769882No Hit
CCCGGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG411970.22670889042020248No Hit
CCCTGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG406830.22388032596949045No Hit
CACGACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC391740.21557623305874246No Hit
CCATGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG373980.20580282748585416No Hit
CAATTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT363120.19982652205108123No Hit
CCTCGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG351630.19350352486456734No Hit
CCTAATGATTAATAGGGATAGTCGGGGGCATTCGTATTCAATTGTCAGAG347540.19125278000009024No Hit
CAAATACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT331330.1823323461973583No Hit
CCTCTGGCTGATCATTAGCTTCTTAGAGGGACTATTGGCATAAAGCCAAT321750.1770604303534242No Hit
CCCAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG310790.17102909448186698No Hit
CACGTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT310510.170875009258871No Hit
CAGTTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT305320.1680189295897668No Hit
CCAGGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG301500.16591676690460727No Hit
ACGGTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT300110.16515184383330575No Hit
AACCTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA294460.16204262415499388No Hit
CCAAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG289030.15905447143760743No Hit
CCCCGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG275860.1518069629131176No Hit
CAAGTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT273870.1507118572211104No Hit
CAAAACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC269410.14825749974053148No Hit
CCGTGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG265910.14633143445308164No Hit
AGCCTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA263040.14475206091737278No Hit
CAATACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC261280.14378352522996946No Hit
CAGGTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT259100.1425838617080721No Hit
CCGCGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG257950.1419510116850529No Hit
CCACGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG257430.1416648534137746No Hit
TGCCGAAGGCAGCAGGCGCGCAAATTACCCAATCCCGACACGGGGAGGTA257360.1416263321080256No Hit
AACTTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA254460.14003044944128148No Hit
CCTGGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG249540.13732295195149485No Hit
CCCTATGATTAATAGGGATAGTCGGGGGCATTCGTATTCAATTGTCAGAG246180.13547392927554303No Hit
CAACTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT244760.1346924970732062No Hit
CAGCTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT244090.13432379314675152No Hit
CTGCGAAGGCAGCAGGCGCGCAAATTACCCAATCCCGACACGGGGAGGTA240850.13254080699494084No Hit
GCCTGAAGGCAGCAGGCGCGCAAATTACCCAATCCCGACACGGGGAGGTA240630.1324197400340154No Hit
CCTAAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCG234320.12894731947292729No Hit
CTTAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG220090.12111648831852409No Hit
CAACACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC214450.11801277168389064No Hit
CAGCACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC212210.11678008989992275No Hit
CCTTATGATTAATAGGGATAGTCGGGGGCATTCGTATTCAATTGTCAGAG209930.11552539594124114No Hit
AACGTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA207330.11409460458484982No Hit
CATATACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT205290.11297198367445051No Hit
AGCTTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA199680.10988477617085234No Hit
CAGTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC196370.10806326871329262No Hit
AACATACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA193960.10673703518679145No Hit
CCGAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG188060.10349023941651889No Hit
TGCTGAAGGCAGCAGGCGCGCAAATTACCCAATCCCGACACGGGGAGGTA185510.10208696327851971No Hit
CACTTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT184220.10137707064400249No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)