FastQCFastQC Report
Mon 26 Jun 2023
ERR10323164_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR10323164_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13711067
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG629120.45884102236536367No Hit
CCTTGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG426230.3108656678579428No Hit
CCCGGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG318460.23226492876156174No Hit
ACGGACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC306800.22376084953855158No Hit
CCCTGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG305630.22290752426488764No Hit
CCTAATGATTAATAGGGATAGTCGGGGGCATTCGTATTCAATTGTCAGAG305540.22284188385922116No Hit
CACGACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC290090.21157361421981236No Hit
CCATGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG283320.20663599703801316No Hit
CCTCGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG263060.1918596123846525No Hit
CCTCTGGCTGATCATTAGCTTCTTAGAGGGACTATTGGCATAAAGCCAAT259680.18939445048295658No Hit
CAATTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT248490.1812331600450935No Hit
CCCAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG236960.17282389474137935No Hit
AACCTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA236770.17268532055163904No Hit
CAAATACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT233830.17054106729986807No Hit
CACGTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT223130.1627371524039668No Hit
CCAAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG222880.1625548179437822No Hit
CCAGGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG221960.16188382713030286No Hit
CCCTATGATTAATAGGGATAGTCGGGGGCATTCGTATTCAATTGTCAGAG216020.15755156035631654No Hit
AGCCTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA214300.15629709927024643No Hit
CAGTTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT213370.1556188150783597No Hit
CCCCGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG212160.15473631629106618No Hit
ACGGTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT206660.15072495816700482No Hit
AACTTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA199450.14546643233528067No Hit
CAAGTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT199140.14524033760465177No Hit
CAAAACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC197360.14394211624813735No Hit
CCGTGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG193910.14142590069758976No Hit
CAATACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC192540.1404267078557781No Hit
CCACGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG191830.13990887798885382No Hit
CCGCGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG189320.13807824000860036No Hit
CAGGTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT184710.13471599256279618No Hit
CCTGGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG183030.13349070499035562No Hit
CCTTATGATTAATAGGGATAGTCGGGGGCATTCGTATTCAATTGTCAGAG181340.13225812403950765No Hit
TGCCGAAGGCAGCAGGCGCGCAAATTACCCAATCCCGACACGGGGAGGTA179290.13076298146599385No Hit
CCTAAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCG175430.1279477374007435No Hit
CTTAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG173930.12685373063963584No Hit
CAACTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT172560.1258545377978242No Hit
CTGCGAAGGCAGCAGGCGCGCAAATTACCCAATCCCGACACGGGGAGGTA172300.1256649099592322No Hit
GCCTGAAGGCAGCAGGCGCGCAAATTACCCAATCCCGACACGGGGAGGTA169040.12328726859842491No Hit
CAGCTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT168230.12269650494742677No Hit
AGCTTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA167280.12200363399872527No Hit
AACGTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA166060.12111384183302437No Hit
GTTCATGATTAATAGGGATAGTCGGGGGCATTCGTATTCAATTGTCAGAG165200.12048661128998932No Hit
AACATACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA156980.1144914542391194No Hit
CAACACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC154770.11287961761108745No Hit
CAGCACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC152280.11106356638764875No Hit
CAGTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC148940.10862757799958238No Hit
CATATACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT147770.10777425272591841No Hit
CCATATGATTAATAGGGATAGTCGGGGGCATTCGTATTCAATTGTCAGAG139300.10159676121486388No Hit
ACCATACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA138130.1007434359411999No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)