FastQCFastQC Report
Mon 26 Jun 2023
ERR10323165_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR10323165_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9928952
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG370640.37329216618229194No Hit
CCTTGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG263150.265033006504614No Hit
CACGACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC221620.22320583280088369No Hit
ACGGACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC220600.22217853404870924No Hit
CCCGGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG199920.20135055542619204No Hit
CCTCTGGCTGATCATTAGCTTCTTAGAGGGACTATTGGCATAAAGCCAAT198740.2001621117717157No Hit
CCCTGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG197090.19850030496672758No Hit
CAATTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT187070.18840860546007274No Hit
CCTAATGATTAATAGGGATAGTCGGGGGCATTCGTATTCAATTGTCAGAG185700.18702880223411292No Hit
CCATGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG179240.18052257680367473No Hit
CAGTTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT172020.17325091308730267No Hit
CACGTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT169820.1710351706806519No Hit
CAAATACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT169540.17075316710162364No Hit
CCTCGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG160020.1611650454146621No Hit
CAAAACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC158410.1595435248352495No Hit
CAATACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC155660.15677384682693601No Hit
CCCAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG150060.15113377524637042No Hit
ACGGTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT148700.14976404357680448No Hit
CAGGTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT145920.1469641508993094No Hit
AACCTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA144780.14581599347040855No Hit
CAAGTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT143440.14446640491363036No Hit
CCCTATGATTAATAGGGATAGTCGGGGGCATTCGTATTCAATTGTCAGAG139830.14083057305544433No Hit
GCCTGAAGGCAGCAGGCGCGCAAATTACCCAATCCCGACACGGGGAGGTA139470.1404679970252651No Hit
CCCCGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG139000.13999463387475336No Hit
CCAGGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG138700.13969248718293734No Hit
CCAAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG136750.13772853368613325No Hit
AGCCTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA132500.1334481222187397No Hit
TGCCGAAGGCAGCAGGCGCGCAAATTACCCAATCCCGACACGGGGAGGTA127620.12853320269853252No Hit
AACTTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA127420.1283317715706552No Hit
CAGCTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT125800.1267001794348487No Hit
CAACTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT125590.1264886767505775No Hit
CCGTGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG125070.1259649558180964No Hit
CCACGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG122140.12301398979469333No Hit
CAACACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC121760.12263127065172638No Hit
CAGTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC121730.12260105598254478No Hit
CATATACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT121340.12220826528318396No Hit
CAGCACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC119250.12010330999686572No Hit
CCGCGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG117960.11880407922205687No Hit
CTGCGAAGGCAGCAGGCGCGCAAATTACCCAATCCCGACACGGGGAGGTA115520.1163466194619533No Hit
CCTTATGATTAATAGGGATAGTCGGGGGCATTCGTATTCAATTGTCAGAG112050.11285178939328139No Hit
CCTGGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG111980.11278128849852433No Hit
CTTAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG106150.10690957112089977No Hit
AGCTTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA104220.1049657607368834No Hit
CACTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC103760.10450246914276552No Hit
CCTAAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCG103340.1040794637742231No Hit
AACGTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA102680.10341474105222788No Hit
CATTTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT100140.10085656572818562No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)