FastQCFastQC Report
Mon 26 Jun 2023
ERR10323166_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR10323166_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13294326
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGTACGCCGGTGAAATACCACTACCTTTATCGTTTCTTTACTTAATCA1552521.1678064762365539No Hit
CGGCTACGCCGGTGAAATACCACTACCTTTATCGTTTCTTTACTTAATCA810020.6092975303900325No Hit
TAGGTACGCCGGTGAAATACCACTACCTTTATCGTTTCTTTACTTAATCA555980.4182084898474733No Hit
GAGGTACGCCGGTGAAATACCACTACCTTTATCGTTTCTTTACTTAATCA463510.3486525003223179No Hit
AGGGTACGCCGGTGAAATACCACTACCTTTATCGTTTCTTTACTTAATCA348130.26186359504047063No Hit
CGTTTAAAACTTTCAGCAACGGATCTCTTGGCTCTCGCATCGATGAAGAA250800.1886519105970472No Hit
CGCTTAAAACTTTCAGCAACGGATCTCTTGGCTCTCGCATCGATGAAGAA219440.1650628997664116No Hit
CGTATAAAACTTTCAGCAACGGATCTCTTGGCTCTCGCATCGATGAAGAA206700.15547986411646594No Hit
AGCACATGCTGAATCCGGTGCGCTCTCGACGGCCCTTGAAAATCCAACGG197070.14823617233397166No Hit
AGCGCATGCTGAATCCGGTGCGCTCTCGACGGCCCTTGAAAATCCAACGG190160.1430384661847468No Hit
CACATCGAATCCATTGCATACGACTTTAATGTGGAACGGGGTATTGTAAG189230.14233891962631276No Hit
CAGGTACGCCGGTGAAATACCACTACCTTTATCGTTTCTTTACTTAATCA170500.12825020237957158No Hit
CGTGTAAAACTTTCAGCAACGGATCTCTTGGCTCTCGCATCGATGAAGAA167850.12625687078833484No Hit
AGCTCATGCTGAATCCGGTGCGCTCTCGACGGCCCTTGAAAATCCAACGG150620.11329645444229366No Hit
CGCATAAAACTTTCAGCAACGGATCTCTTGGCTCTCGCATCGATGAAGAA139220.10472136759697333No Hit
CGGTTACGCCGGTGAAATACCACTACCTTTATCGTTTCTTTACTTAATCA138620.10427004723669331No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)