FastQCFastQC Report
Mon 26 Jun 2023
ERR10323169_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR10323169_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18902964
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCTACGCCGGTGAAATACCACTACCTTTATCGTTTCTTTACTTAATCA3204171.695062213523763No Hit
AAGGTACGCCGGTGAAATACCACTACCTTTATCGTTTCTTTACTTAATCA2052271.0856868795814243No Hit
GAGGTACGCCGGTGAAATACCACTACCTTTATCGTTTCTTTACTTAATCA1048150.5544897614998368No Hit
TAGGTACGCCGGTGAAATACCACTACCTTTATCGTTTCTTTACTTAATCA993930.525806429086994No Hit
AGGGTACGCCGGTGAAATACCACTACCTTTATCGTTTCTTTACTTAATCA679150.3592822797525298No Hit
CGGTTACGCCGGTGAAATACCACTACCTTTATCGTTTCTTTACTTAATCA433270.22920744069554383No Hit
CGTTTAAAACTTTCAGCAACGGATCTCTTGGCTCTCGCATCGATGAAGAA297000.1571182170161251No Hit
TTTCAGGGTTTGAGTAAGAGCATAGCTGTTGGGACCCGAAAGATGGTGAA269450.14254378308079094No Hit
CGTATAAAACTTTCAGCAACGGATCTCTTGGCTCTCGCATCGATGAAGAA252300.1334711318288497No Hit
CAGGTACGCCGGTGAAATACCACTACCTTTATCGTTTCTTTACTTAATCA248960.13170421315937544No Hit
CGCTTAAAACTTTCAGCAACGGATCTCTTGGCTCTCGCATCGATGAAGAA236020.12485872585907692No Hit
ACCGTACGCCGGTGAAATACCACTACCTTTATCGTTTCTTTACTTAATCA219580.11616167707879038No Hit
CGGGTACGCCGGTGAAATACCACTACCTTTATCGTTTCTTTACTTAATCA216020.11427837454485974No Hit
AGGTTACGCCGGTGAAATACCACTACCTTTATCGTTTCTTTACTTAATCA210490.11135290740647869No Hit
AGCGCATGCTGAATCCGGTGCGCTCTCGACGGCCCTTGAAAATCCAACGG205990.10897232836077982No Hit
TTGGTACGCCGGTGAAATACCACTACCTTTATCGTTTCTTTACTTAATCA197140.10429052290423872No Hit
AGCACATGCTGAATCCGGTGCGCTCTCGACGGCCCTTGAAAATCCAACGG196290.10384085797338449No Hit
CGTGTAAAACTTTCAGCAACGGATCTCTTGGCTCTCGCATCGATGAAGAA192900.10204748842562467No Hit
CACATCGAATCCATTGCATACGACTTTAATGTGGAACGGGGTATTGTAAG191820.10147614945465695No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)