FastQCFastQC Report
Mon 26 Jun 2023
ERR10323170_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR10323170_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28423411
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA902650.3175727220072215No Hit
AGCCTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA858130.3019095772847249No Hit
AACTTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA727790.2560530120751517No Hit
CCTAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG654560.23028903884899668No Hit
AACATACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA628900.22126126945143917No Hit
AGCTTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA627830.22088481920765948No Hit
CAATTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT602120.21183945867721504No Hit
CAACTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT598970.21073121730534028No Hit
CAAATACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT572860.20154512771180066No Hit
ACCATACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA548280.1928973267846002No Hit
AACGTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA542030.1906984351737376No Hit
CACGTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT514490.1810092391796326No Hit
ACCCTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA461640.16241541171817836No Hit
AATCTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA450250.15840815164654237No Hit
CCTAAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCG447070.15728935559493545No Hit
CAAGTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT423510.14900041377862777No Hit
ACGGTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT423110.14885968471553254No Hit
AGCATACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA421390.14825454974422317No Hit
CAGCTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT421380.1482510315176458No Hit
CAGTTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT414790.14593252020315226No Hit
ACCGTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA414360.1457812364603249No Hit
CCTTGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG406540.1430299832768136No Hit
CAGGTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT360910.12697631540422785No Hit
ATCCTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA352600.12405266911842494No Hit
CATATACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT334380.11764246029443827No Hit
CAATACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC333310.11726601005065859No Hit
CCCGGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG324290.11409256967786166No Hit
CCCTGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG311850.10971589581560075No Hit
CCCAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG307870.10831564163780343No Hit
CACATACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT303740.10686261406134541No Hit
CCATGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG296110.10417820718280435No Hit
CCTCGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG295260.10387915792372703No Hit
CCAGTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT293410.10322828600691168No Hit
CACGACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC292160.10278850768473918No Hit
CACTTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT284940.10024834809587069No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)