FastQCFastQC Report
Mon 26 Jun 2023
ERR10323172_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR10323172_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25533382
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA790720.3096808718876332No Hit
AGCCTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA741630.29045505996816245No Hit
AACTTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA636090.24912093509586783No Hit
CCTAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG609790.2388206936315761No Hit
CAACTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT580060.2271771126911429No Hit
CAATTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT569770.22314709426271853No Hit
AACATACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA553730.21686512190198698No Hit
AGCTTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA549040.21502831078154863No Hit
CAAATACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT546580.21406486614268333No Hit
CACGTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT523960.20520587519506817No Hit
CAAGTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT498300.19515628599454626No Hit
AACGTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA486550.19055446708939694No Hit
ACCATACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA471000.18446440036811418No Hit
CCTAAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCG459120.1798116677218866No Hit
ACGGTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT453530.17762237685552193No Hit
CAGTTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT400000.15665766485614793No Hit
CAGCTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT398460.15605453284645174No Hit
CAGGTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT397020.15549056525296961No Hit
ACCCTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA395640.1549500963092159No Hit
AGCATACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA390550.15295662752392142No Hit
AATCTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA384550.15060676255107922No Hit
CCTTGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG378650.14829606199445103No Hit
ACCGTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA369590.1447477658854593No Hit
CATATACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT332170.13009244133816664No Hit
CAATACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC322700.12638357112269735No Hit
CCCGGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG312970.12257287342507155No Hit
ATCCTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA308320.12075172807111882No Hit
CACATACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT296870.11626740241461159No Hit
CCCTGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG291820.11428959939580272No Hit
CACGACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC287070.11242928962563595No Hit
CCCAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG285890.11196714951431032No Hit
CCTCGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG280780.10996584784577304No Hit
CCATGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG280170.10972694490686741No Hit
CACTTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT278960.10925305547067757No Hit
CATTTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT270850.10607682131571916No Hit
CCAGTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT270810.10606115554923354No Hit
CCTTAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCG269820.10567342782871458No Hit
CATCTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT263910.103358810830465No Hit
TAAGTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT260050.10184706436460317No Hit
ACGGACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC257200.10073087850250312No Hit

[FAIL]Adapter Content

Adapter graph