FastQCFastQC Report
Mon 26 Jun 2023
ERR10323173_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR10323173_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12561195
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG578010.46015526389009964No Hit
CCTAAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCG460540.36663709145507256No Hit
CCTTGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG363340.28925591872429335No Hit
AACCTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA354250.2820193460892853No Hit
AGCCTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA338430.2694250029555309No Hit
CCTCGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG268630.2138570414677903No Hit
CCCTGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG261340.20805345351298182No Hit
AACTTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA247700.1971946140474692No Hit
CCCGGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG246950.19659753709738606No Hit
CCATGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG240920.19179703841871734No Hit
CCTTAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCG240030.19108850710461864No Hit
AGCTTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA225080.17918677323296073No Hit
CCCCGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG219440.17469675456833525No Hit
CCCAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG210830.16784231118138043No Hit
CCACGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG208790.16621826187715422No Hit
CAACTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT198590.15809801535602305No Hit
CCAAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG190220.15143463659309486No Hit
CCGCGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG190100.15133910428108155No Hit
CCTCAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCG179580.1429641049279149No Hit
AACGTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA177790.1415390812737164No Hit
AACATACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA174730.13910300731737707No Hit
CCGTGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG174560.1389676698753582No Hit
CCAGGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG170620.13583102563092125No Hit
ACCCTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA161660.12869794633392762No Hit
CAATTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT160020.12739233806974573No Hit
CTTAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG156240.12438307024132655No Hit
CCCTAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCG148500.11822123611646822No Hit
CCATAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCG148280.11804609354444381No Hit
CAGTTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT145510.11584088934213663No Hit
AGCATACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA145400.11575331805612445No Hit
ACCATACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA144930.11537914983407233No Hit
CACGTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT137890.1097745875292916No Hit
ACCGTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA136790.1088988746691696No Hit
CAAATACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT133660.1064070735308225No Hit
CCTGGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG132230.10526864681266392No Hit
CCACAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCG129030.10272111849230905No Hit
ACGGTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT127330.10136774407212054No Hit
AATCTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA127250.10130405586411166No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)