FastQCFastQC Report
Mon 26 Jun 2023
ERR10323174_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR10323174_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5326881
Total Bases538 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG223790.42011450978536974No Hit
CCTAAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCG169460.31812236841784153No Hit
CCTTGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG143560.26950104573389194No Hit
AACCTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA122690.23032239691481748No Hit
AGCCTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA119770.22484076516821005No Hit
CCCTGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG106880.2006427400950012No Hit
CCTCGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG104650.1964564254392017No Hit
CCCGGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG96460.18108157475265543No Hit
CCATGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG94560.1775147595750684No Hit
CCTTAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCG92280.17323458136196396No Hit
AACTTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA89610.16822226740188115No Hit
CCCCGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG85320.16016877418511885No Hit
AGCTTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA82290.15448064261244057No Hit
CCCAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG81870.15369218873107923No Hit
CCACGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG81100.15224668994858342No Hit
CCGCGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG76670.14393037877136736No Hit
CCAAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG71660.13452525032941415No Hit
CCGTGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG69750.13093966244036614No Hit
CAACTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT69290.13007611771316085No Hit
CCTCAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCG67160.12607753017197118No Hit
CCAGGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG64020.12018289877322207No Hit
AACGTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA63560.11931935404601679No Hit
AACATACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA63160.11856844558757743No Hit
CAATTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT61260.1150016304099904No Hit
CCCTAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCG60570.11370631331918246No Hit
CACGTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT60450.11348104078165065No Hit
ACCCTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA59840.11233590538253059No Hit
CTTAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG59290.11130340625217647No Hit
CAGTTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT57110.10721095515368187No Hit
CCATAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCG56880.10677918279007922No Hit
ACGGTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT54770.10281814067181151No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)