FastQCFastQC Report
Mon 26 Jun 2023
ERR10323175_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR10323175_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12713212
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG482230.379314055330785No Hit
CCTAAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCG390530.3071843685136376No Hit
CCTTGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG307830.2421339312205287No Hit
AACCTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA297090.23368602678850947No Hit
AGCCTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA284050.22342898081145818No Hit
CCCTGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG227800.17918367128621784No Hit
CCTCGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG220930.17377984414953515No Hit
CCTTAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCG214290.16855693116735565No Hit
CCCGGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG212600.16722760542339732No Hit
AACTTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA212230.1669365696096313No Hit
CCATGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG200800.15794592271410246No Hit
AGCTTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA194940.15333654469067298No Hit
CCCCGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG186820.1469494884534294No Hit
CCCAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG181180.14251315875169862No Hit
CCACGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG175240.13784085406583324No Hit
CCAAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG157740.12407564665798068No Hit
CCTCAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCG156950.12345424586642621No Hit
CCGCGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG156030.12273058924841339No Hit
CAACTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT154960.12188894513833326No Hit
AACGTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA153030.12037083940706723No Hit
AACATACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA150390.11829425954668263No Hit
ACCCTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA146330.11510073142806082No Hit
CCGTGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG144200.11342530904070505No Hit
CCAGGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG140350.11039696341097749No Hit
CCCTAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCG140320.11037336591256403No Hit
CAATTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT139350.10961038013052878No Hit
CACGTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT134950.10614941369655442No Hit
CTTAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG132060.10387618801605762No Hit
CAGTTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT130960.10301094640756403No Hit
CCATAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCG130810.10289295891549673No Hit
AGCATACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA127810.10053320907415057No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)