FastQCFastQC Report
Mon 26 Jun 2023
ERR10323178_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR10323178_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27481499
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCTACGCCGGTGAAATACCACTACCTTTATCGTTTCTTTACTTAATCA1279450.465567762515429No Hit
AAGGTACGCCGGTGAAATACCACTACCTTTATCGTTTCTTTACTTAATCA709470.25816277343532096No Hit
CCCATGCCTGGGGAGTGTTTATAGCCCTTGTTGTAATACGTCCACTGGGG660160.24021979295961987No Hit
CCCGTGCCTGGGGAGTGTTTATAGCCCTTGTTGTAATACGTCCACTGGGG564730.20549461293941793No Hit
CGCTTAAAACTTTCAGCAACGGATCTCTTGGCTCTCGCATCGATGAAGAA453140.16488911321758684No Hit
AGCACATGCTGAATCCGGTGCGCTCTCGACGGCCCTTGAAAATCCAACGG450750.16401943722211076No Hit
CGTTTAAAACTTTCAGCAACGGATCTCTTGGCTCTCGCATCGATGAAGAA447370.162789518868676No Hit
CCCTTGCCTGGGGAGTGTTTATAGCCCTTGTTGTAATACGTCCACTGGGG438140.1594308956727579No Hit
AGCGCATGCTGAATCCGGTGCGCTCTCGACGGCCCTTGAAAATCCAACGG409980.1491840019352656No Hit
CCAGTGCCTGGGGAGTGTTTATAGCCCTTGTTGTAATACGTCCACTGGGG406850.14804505387424463No Hit
CGTATAAAACTTTCAGCAACGGATCTCTTGGCTCTCGCATCGATGAAGAA400610.14577443537559578No Hit
AGCTCATGCTGAATCCGGTGCGCTCTCGACGGCCCTTGAAAATCCAACGG340800.12401070261851438No Hit
CCCAGCCTGGGGAGTGTTTATAGCCCTTGTTGTAATACGTCCACTGGGGA336120.12230773874452772No Hit
CGTGTAAAACTTTCAGCAACGGATCTCTTGGCTCTCGCATCGATGAAGAA317620.11557593710590532No Hit
TAGGTACGCCGGTGAAATACCACTACCTTTATCGTTTCTTTACTTAATCA309180.11250478003401489No Hit
CGCATAAAACTTTCAGCAACGGATCTCTTGGCTCTCGCATCGATGAAGAA307510.11189709848069058No Hit
CGCCTAAAACTTTCAGCAACGGATCTCTTGGCTCTCGCATCGATGAAGAA301570.10973564433293832No Hit
CCCCTGCCTGGGGAGTGTTTATAGCCCTTGTTGTAATACGTCCACTGGGG276410.10058039410441184No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)