FastQCFastQC Report
Thu 29 Jun 2023
ERR10323184_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR10323184_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15333409
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG1472790.9605104774809047No Hit
CCTTGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG1046200.682300980819073No Hit
CCTCGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG756780.4935497383523781No Hit
CCATGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG658810.42965657539037794No Hit
CCCGGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG646400.42156313706886706No Hit
CCCTGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG640030.4174088097434824No Hit
CCACGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG573010.3737003297831552No Hit
CCCCGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG550970.359326487671463No Hit
CCGCGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG526740.3435243917383277No Hit
CCCAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG502070.32743534069951435No Hit
CCGTGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG490430.31984407381294006No Hit
CCAAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG469010.3058745775319761No Hit
CCAGGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG452870.2953485425191489No Hit
CCTGGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG335640.2188945719767861No Hit
CCGAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG333870.2177402298471266No Hit
CCTAAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCG333230.21732284060250398No Hit
CTTAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG316580.2064641985353681No Hit
ACGGACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC315030.20545333395854765No Hit
CACGACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC278420.18157736482474315No Hit
CCGGGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG260590.16994916133783428No Hit
AGCCTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA257520.16794699730503504No Hit
AACCTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA257350.16783612828693215No Hit
CCTAATGATTAATAGGGATAGTCGGGGGCATTCGTATTCAATTGTCAGAG244280.15931225730690415No Hit
CCTAATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGGG216270.1410449561477164No Hit
CAATACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC211440.1378949716922049No Hit
TGCCGAAGGCAGCAGGCGCGCAAATTACCCAATCCCGACACGGGGAGGTA207010.13500585551458258No Hit
CCTATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGGGC206210.13448411895880424No Hit
CAGTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC204050.13307543025820287No Hit
CACTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC196580.12820371516862297No Hit
AACTTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA195070.12721893741959142No Hit
CCTTAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCG191370.12480590584911677No Hit
CCTTATGATTAATAGGGATAGTCGGGGGCATTCGTATTCAATTGTCAGAG184430.12027984122774003No Hit
CCCGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGGG180570.11776246234610974No Hit
AGCTTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA179350.11696681409854781No Hit
CCCGATGATTAATAGGGATAGTCGGGGGCATTCGTATTCAATTGTCAGAG175560.11449508716554811No Hit
CTATGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG175080.11418204523208114No Hit
CTAAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG162150.10574947814931436No Hit
CCCAATGATTAATAGGGATAGTCGGGGGCATTCGTATTCAATTGTCAGAG160180.10446470188071029No Hit
CCTTATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGGG157280.10257340686601393No Hit
CAGCACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC156510.10207123543107732No Hit
CCCCATGATTAATAGGGATAGTCGGGGGCATTCGTATTCAATTGTCAGAG156450.10203210518939394No Hit
CACCACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC154620.1008386328180511No Hit
CCGGACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC154420.10070819867910652No Hit
CAACACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC154410.10070167697215927No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)