FastQCFastQC Report
Mon 26 Jun 2023
ERR10323185_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR10323185_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25705886
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG2074630.8070641875561106No Hit
CCTTGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG1535700.5974118145548456No Hit
CCTCGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG1095890.42631870381748366No Hit
CCCTGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG996820.3877788923517361No Hit
CCATGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG968220.3766530358066631No Hit
CCCGGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG945760.3679157372751128No Hit
CCCCGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG848240.3299789005521926No Hit
CCACGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG832410.323820777856091No Hit
CCGCGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG775910.30184137594012517No Hit
CCCAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG758520.295076388341565No Hit
CCGTGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG709990.2761974436516213No Hit
CCAAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG682240.2654022506751956No Hit
CCAGGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG637710.24807936983770956No Hit
CACGACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC616230.2397233069500114No Hit
ACGGACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC611600.2379221630407915No Hit
AACCTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA578840.22517800008916244No Hit
AGCCTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA575200.22376198198342587No Hit
CAATACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC546120.21244939777605798No Hit
CAGTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC513030.19957685955660115No Hit
CTTAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG496870.19329036159267182No Hit
CCTAAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCG491420.19117022459369812No Hit
CCGAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG474720.18467365800968696No Hit
CCTGGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG463860.18044894465026415No Hit
CACTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC458690.17843773212096248No Hit
AACTTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA419620.16323887844208132No Hit
CCTAATGATTAATAGGGATAGTCGGGGGCATTCGTATTCAATTGTCAGAG412760.16057022893511627No Hit
AGCTTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA388450.15111325087180422No Hit
CAACACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC378330.1471764093250861No Hit
CAGCACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC376160.14633224468512776No Hit
CAAAACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC366080.14241096377693419No Hit
TGCCGAAGGCAGCAGGCGCGCAAATTACCCAATCCCGACACGGGGAGGTA360130.14009631879640327No Hit
CACCACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC350040.13617114772857858No Hit
CACAACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC328320.12772172100973295No Hit
CATTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC326640.1270681741917007No Hit
AACGTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA323700.12592446726014422No Hit
CCTAATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGGG321070.12490135527715325No Hit
CATAACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC312710.12164918182551654No Hit
CCTTATGATTAATAGGGATAGTCGGGGGCATTCGTATTCAATTGTCAGAG307750.11971966264846892No Hit
AACATACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA302330.11761119612838866No Hit
CCTTAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCG299760.11661142510318455No Hit
CATCACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC299710.11659197430502882No Hit
CCTATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGGGC294240.11446405698679284No Hit
CCCGATGATTAATAGGGATAGTCGGGGGCATTCGTATTCAATTGTCAGAG293550.11419563597224386No Hit
CGCTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC293310.11410227214109639No Hit
CCGGGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG292850.11392332479806377No Hit
CTATGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG291360.11334369101302325No Hit
CCCAATGATTAATAGGGATAGTCGGGGGCATTCGTATTCAATTGTCAGAG283590.11032103697962403No Hit
CCGGACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC281390.10946520186077227No Hit
ACCCTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA279520.10873774200974827No Hit
CCCGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGGG274410.10674987043823349No Hit
CCCCATGATTAATAGGGATAGTCGGGGGCATTCGTATTCAATTGTCAGAG273450.10637641511364362No Hit
CGGTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC265280.10319815469499866No Hit
CAGGACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC265110.1031320219812692No Hit
CTAAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG263670.1025718389943844No Hit
CAATTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT259800.10106634721713152No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)