FastQCFastQC Report
Mon 26 Jun 2023
ERR10323186_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR10323186_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3247049
Total Bases327.9 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGTACGCCGGTGAAATACCACTACCTTTATCGTTTCTTTACTTAATCA205010.6313732869445456No Hit
CGGCTACGCCGGTGAAATACCACTACCTTTATCGTTTCTTTACTTAATCA200860.6185924511764375No Hit
TAGGTACGCCGGTGAAATACCACTACCTTTATCGTTTCTTTACTTAATCA104100.32059879601447344No Hit
AGGGTACGCCGGTGAAATACCACTACCTTTATCGTTTCTTTACTTAATCA102980.3171495102168153No Hit
GAGGTACGCCGGTGAAATACCACTACCTTTATCGTTTCTTTACTTAATCA95740.29485234131052535No Hit
CGGTTACGCCGGTGAAATACCACTACCTTTATCGTTTCTTTACTTAATCA67220.20701874224873107No Hit
AGGTTACGCCGGTGAAATACCACTACCTTTATCGTTTCTTTACTTAATCA53560.1649497743951508No Hit
CCTAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG44360.1366163553429591No Hit
CCCCCGAGACTATGCACTCTGAACCTGTGGTAGGTCAGCATCAGTTTTCG43310.1333826499076546No Hit
CGTTTAAAACTTTCAGCAACGGATCTCTTGGCTCTCGCATCGATGAAGAA40810.12568335125216773No Hit
CAGGTACGCCGGTGAAATACCACTACCTTTATCGTTTCTTTACTTAATCA38660.11906195440844904No Hit
CGTATAAAACTTTCAGCAACGGATCTCTTGGCTCTCGCATCGATGAAGAA34110.10504923085546292No Hit
CCTTGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG34060.10489524488235319No Hit
CGCCCGAGACTATGCACTCTGAACCTGTGGTAGGTCAGCATCAGTTTTCG33510.10320139917814605No Hit
GTAGATGCTGAATCCGGTGCGCTCTCGACGGCCCTTGAAAATCCAACGGA32550.10024486849443912No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)