FastQCFastQC Report
Mon 26 Jun 2023
ERR10323187_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR10323187_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5910777
Total Bases596.9 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCTACGCCGGTGAAATACCACTACCTTTATCGTTTCTTTACTTAATCA551700.9333798246829478No Hit
AAGGTACGCCGGTGAAATACCACTACCTTTATCGTTTCTTTACTTAATCA504300.853187322072885No Hit
AGGGTACGCCGGTGAAATACCACTACCTTTATCGTTTCTTTACTTAATCA257130.4350189492853478No Hit
TAGGTACGCCGGTGAAATACCACTACCTTTATCGTTTCTTTACTTAATCA256090.4332594513377852No Hit
GAGGTACGCCGGTGAAATACCACTACCTTTATCGTTTCTTTACTTAATCA237390.40162232478065063No Hit
CGGTTACGCCGGTGAAATACCACTACCTTTATCGTTTCTTTACTTAATCA158030.2673590967820305No Hit
AGGTTACGCCGGTGAAATACCACTACCTTTATCGTTTCTTTACTTAATCA131960.22325322034649592No Hit
CGTTTAAAACTTTCAGCAACGGATCTCTTGGCTCTCGCATCGATGAAGAA102420.17327671133592082No Hit
CAGGTACGCCGGTGAAATACCACTACCTTTATCGTTTCTTTACTTAATCA101900.17239696236213953No Hit
CCCCCGAGACTATGCACTCTGAACCTGTGGTAGGTCAGCATCAGTTTTCG89450.15133374173987615No Hit
CGTATAAAACTTTCAGCAACGGATCTCTTGGCTCTCGCATCGATGAAGAA82430.13945713059382886No Hit
CGCCCGAGACTATGCACTCTGAACCTGTGGTAGGTCAGCATCAGTTTTCG68250.11546705280879317No Hit
GTAGATGCTGAATCCGGTGCGCTCTCGACGGCCCTTGAAAATCCAACGGA66260.11210032115913017No Hit
CCCGTCGAATCCATTGCATACGACTTTAATGTGGAACGGGGTATTGTAAG63790.10792151353366909No Hit
CCCGTAGGTCAGCATCAGTTTTCGGGGGCGGAAAAAGAATAAGGGAAGGT63160.10685566381543408No Hit
CGCTTAAAACTTTCAGCAACGGATCTCTTGGCTCTCGCATCGATGAAGAA62340.10546836735677899No Hit
TTGGTACGCCGGTGAAATACCACTACCTTTATCGTTTCTTTACTTAATCA61060.10330283142131735No Hit
CCCGCGAGACTATGCACTCTGAACCTGTGGTAGGTCAGCATCAGTTTTCG60580.10249075544551925No Hit
GGTATACGCCGGTGAAATACCACTACCTTTATCGTTTCTTTACTTAATCA60470.10230465470106552No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)