FastQCFastQC Report
Mon 26 Jun 2023
ERR10323190_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR10323190_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2222439
Total Bases224.4 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA63700.286622040019996No Hit
AGCCTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA58260.26214442781106706No Hit
AACTTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA55870.2513904768589824No Hit
CACGACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC51180.23028753545091674No Hit
CAATACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC45810.20612489251673496No Hit
AGCTTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA45720.20571993202063138No Hit
CAATTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT45710.20567493640995324No Hit
AACATACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA44470.20009548068585908No Hit
ACGGACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC43940.19771071331991563No Hit
CAAATACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT43390.19523595473261585No Hit
CAAAACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC42180.18979148584055625No Hit
AACGTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA41860.18835162629885455No Hit
CCTAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG41360.18610184576494565No Hit
ACCATACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA38660.17395303088183747No Hit
CACGTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT37160.16720368928011073No Hit
CAACTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT37050.16670873756265076No Hit
CAACACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC36020.16207418966279838No Hit
CAAGTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT34320.15442493584750808No Hit
ACCGTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA33130.14907045817680484No Hit
ACCCTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA31140.14011633165184736No Hit
AATCTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA30270.13620171352284585No Hit
CAGTTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT30070.13530180130928227No Hit
AGCATACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA29580.13309701638605154No Hit
ACGGTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT29100.13093722707349897No Hit
CAGTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC28420.12787752554738285No Hit
CACTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC28200.1268876221124629No Hit
CAGCTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT27410.12333296886888684No Hit
CAGCACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC26780.1204982453961616No Hit
CAGGTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT26070.11730355703801094No Hit
CCTTGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG25900.11653863165648191No Hit
CACAACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC25770.11595368871766559No Hit
CATATACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT25440.11446883356528571No Hit
TAAGACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC25030.11262401352748039No Hit
ATCCTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA23810.10713454902474265No Hit
CCAGTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAAT23570.10605465436846635No Hit
CCAGTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA22680.10205004501810848No Hit
CCTAATGATTAATAGGGATAGTCGGGGGCATTCGTATTCAATTGTCAGAG22460.10106014158318855No Hit
CATCACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC22330.10047519864437224No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)