FastQCFastQC Report
Mon 26 Jun 2023
ERR10323205_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR10323205_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13593841
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCTACGCCGGTGAAATACCACTACCTTTATCGTTTCTTTACTTAATCA797190.5864346949475133No Hit
CGTTTAAAACTTTCAGCAACGGATCTCTTGGCTCTCGCATCGATGAAGAA355610.2615964097270227No Hit
AAGGTACGCCGGTGAAATACCACTACCTTTATCGTTTCTTTACTTAATCA335710.246957427264303No Hit
CGTATAAAACTTTCAGCAACGGATCTCTTGGCTCTCGCATCGATGAAGAA275370.20256967843010668No Hit
CGGTTACGCCGGTGAAATACCACTACCTTTATCGTTTCTTTACTTAATCA219250.16128627663071826No Hit
CCCCTGGGGAGTGTTTATAGCCCTTGTTGTAATACGTCCACTGGGGACTG215120.15824813604925936No Hit
CCCATGCCTGGGGAGTGTTTATAGCCCTTGTTGTAATACGTCCACTGGGG213850.15731388942977928No Hit
CCCGTGCCTGGGGAGTGTTTATAGCCCTTGTTGTAATACGTCCACTGGGG209560.15415804848681106No Hit
CGCTTAAAACTTTCAGCAACGGATCTCTTGGCTCTCGCATCGATGAAGAA209190.15388586640082078No Hit
CCCTTGCCTGGGGAGTGTTTATAGCCCTTGTTGTAATACGTCCACTGGGG194450.14304272059677614No Hit
CCAGTGCCTGGGGAGTGTTTATAGCCCTTGTTGTAATACGTCCACTGGGG182080.13394301139758807No Hit
TAGGTACGCCGGTGAAATACCACTACCTTTATCGTTTCTTTACTTAATCA181780.13372232321975813No Hit
CGTGTAAAACTTTCAGCAACGGATCTCTTGGCTCTCGCATCGATGAAGAA168890.12424008784566482No Hit
CCCCTGCCTGGGGAGTGTTTATAGCCCTTGTTGTAATACGTCCACTGGGG167960.12355595449439198No Hit
AGGGTACGCCGGTGAAATACCACTACCTTTATCGTTTCTTTACTTAATCA167090.12291595877868514No Hit
GAGGTACGCCGGTGAAATACCACTACCTTTATCGTTTCTTTACTTAATCA162880.11981896801647157No Hit
CGTTAAAACTTTCAGCAACGGATCTCTTGGCTCTCGCATCGATGAAGAAC162370.11944379811416067No Hit
CCAGTGGGGAGTGTTTATAGCCCTTGTTGTAATACGTCCACTGGGGACTG161960.11914219093779309No Hit
AGCACATGCTGAATCCGGTGCGCTCTCGACGGCCCTTGAAAATCCAACGG160580.11812702531977533No Hit
CCCATGGGGAGTGTTTATAGCCCTTGTTGTAATACGTCCACTGGGGACTG152940.11250683305770606No Hit
CCCCGGGGAGTGTTTATAGCCCTTGTTGTAATACGTCCACTGGGGACTGA152590.11224936351690445No Hit
CGCCTAAAACTTTCAGCAACGGATCTCTTGGCTCTCGCATCGATGAAGAA149230.10977765592520908No Hit
ACCGTACGCCGGTGAAATACCACTACCTTTATCGTTTCTTTACTTAATCA142140.10456205865582804No Hit
AGCGCATGCTGAATCCGGTGCGCTCTCGACGGCCCTTGAAAATCCAACGG138670.102009432065595No Hit
AGCTCATGCTGAATCCGGTGCGCTCTCGACGGCCCTTGAAAATCCAACGG138300.10173724997960473No Hit
CCCGTAGGTCAGCATCAGTTTTCGGGGGCGGAAAAAGAATAAGGGAAGGT137700.10129587362394485No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)