FastQCFastQC Report
Mon 26 Jun 2023
ERR10323209_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR10323209_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15920897
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG752850.4728690852029254No Hit
CCTTGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG559500.35142492285453514No Hit
CCTCGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG399040.2506391442642962No Hit
CCCTGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG352330.22130034507477814No Hit
CCATGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG347870.21849899537695647No Hit
CCCGGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG331500.2082169113963868No Hit
CCACGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG302790.1901840078483015No Hit
CCCCGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG302600.19006466783875306No Hit
ACGGACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC285530.17934291013879433No Hit
CCGCGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG285150.17910423011969742No Hit
CCCAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG274730.17255937275393465No Hit
AGCCTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA266470.16737122286514383No Hit
CACGACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC264630.16621550908846405No Hit
AACCTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA262200.16468921317687063No Hit
CCGTGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG261920.164513343689115No Hit
CCAAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG248110.15583920931088244No Hit
CCAGGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG231070.14513629477032608No Hit
CCTAAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCG209920.1318518673916426No Hit
CAATACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC208920.13122376207822964No Hit
CAGTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC206660.12980424406991642No Hit
AACTTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA204130.1282151376269817No Hit
CACTACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTAAAAATC186760.11730494833299908No Hit
AGCTTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTA185930.11678362092286634No Hit
CCTAATGATTAATAGGGATAGTCGGGGGCATTCGTATTCAATTGTCAGAG179590.11280143323582836No Hit
CCGAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG177710.11162059524661204No Hit
CTTAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG176180.11065959411709027No Hit
TGCCGAAGGCAGCAGGCGCGCAAATTACCCAATCCCGACACGGGGAGGTA165240.10378812198835279No Hit
CCTGGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGG164350.10322910825941527No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)